同源的

Coli XL-1 strain, purposed cassette recombination plasmids pNB0098-K and pNB0097-K were constructed, in which the purposed gene was linked with plasmid pUC18, and the marker gene kanamycin was inserted in the purposed gene. The linear cassette DNA fragment was mixed in the medium of cultured competent cells of meningococcus BT878. The function of the purposed gene was inhibited by the homologous replace of casstte gene.

将线性的重组质粒片段置于培养基中，与感受态的脑膜炎双球菌混合培养，重组质粒中的同源性片段被脑膜炎双球菌细胞识别和摄取，整合到染色体上，目的基因被带有卡那霉素基因插入的同源性重组片段代替，导致失活，在选择性培养基上筛选出基因被敲除的突变株。

90In this paper, the 10kDa sulfur-rich prolamin gene was amplified by PCR, cloned and sequenced from Chi-nese rice. The sequence analysis showed that the amplified fragment was 380bp long, 94. 5 % homologousto the nucleic acid sequence reported by Masumura et al.

对扩增产物的核苷酸序列分析表明：本扩增产物长 380bp；与 Masumura等人[1] 发表的序列相比，有94.5％的同源性，与我们以前发表的中花10号水稻10kDa富硫醇溶蛋白基因的序列相比，有99.5％的同源性。

The Ids RNA or siRNA obtained by said invention is mixed with hepatitis B virus expression vector in proper proportion, and injected into the mouse body by vane caudalis hydraulic pressure method, and its result shows that the non-homologous sequence IdsRNA and siRNA basically do not inhibit the replication of hepatitis B virus in mouse body, and these RNA molecules are mixed with hepatitis B virus gene.

将本发明得到的ldsRNA或siRNA以适当的比例与乙型肝炎病毒表达载体混合，并用尾静脉液压法注射到小鼠体内。结果显示非同源性序列ldsRNA和siRNA基本不抑制乙型肝炎病毒在小鼠体内的复制，而与乙肝病毒基因同源性的ldsRNA和siRNA可以有效抑制乙型肝炎病毒在小鼠体内的感染复制，抑制率高达90％以上。

The IL-2 gene is 468bp, encoding a putative 155 amino acid protein, the DNA sepuencehomology of this caprine IL-2 and the corresponding caprine(AF535145) cytokine is 100.0％, thegene homology to Qihua Yings is 99.1％, the first 20 amino terminal aa sequence compose ahydrophobic signal sequence.

经SignalP 3.0分析表明，前23个氨基酸为信号肽序列；IL-2基因开放阅读框架共有468bp，编码一条155个氨基酸的多肽，与GenBank发表的山羊白细胞介素-2基因序列(AF535145)比较核苷酸／氨基酸同源性为100.0％，与应琦华等的山羊IL-2序列的同源性为99.1％，前20个氨基酸为该蛋白的信号肽序列。

The genetic DNA was extracted from the blood of 6 A. forsteri which sex was unknown. The PCR amplified fragments with primers P2/P8 were cloned in T-Vector. Taking homologous sequence of Circaetus gallicus as reference, the CHD gene sequence was compared to the homologues in GenBank to identify its sex.

方法］采用苯酚：氯仿抽提法提取6只未知性别的帝企鹅血液中的基因组DNA，运用P2/P8引物扩增CHD基因片段，将PCR产物克隆到T-Vector，利用NCBI的Blast程序，以短趾鹰同源序列为比对参照，将帝企鹅CHD基因片段序列与GenBank中的基因片段序列进行同源性比较分析，鉴定帝企鹅的性别。

Three major clusters of numt sequences ( cyt b numt、 ATP8+ATP6 numt and ND4+ tRNA-His+ tRNA-Ser numt) were particularity amplified and sequenced from 10 species within Galliformes and A.galericalata. Comparison numts sequences with homologous mitochondrial partial genes get the different evolutionary characteristics and evolutionary speed compared to mtDNA, then examine the age of insertion of numts using the sequence divergence of numt from the mtDNA. Finally, joined Coturnix japonica、 Coturnix chinensis、 Lophura leucomelana、 Lophura swinhoii and A.albifrons mitochondrial sequences in GenBank, we study the importance function to phylogenetic research of mitochondrial genes and homologous numts.The evolutionary rate of Numts is lower than their authentic sequences of mtDNA, cyt b and ND4 gene evolve faster than homologous numts 2.5 times; ATP8+ATP6 gene evolve faster than numts 18.9 times in Galliformes. Numts show less codon position bias and transition bias under not phylogenetic pressure.

根据2004年3月GenBank登录的红原鸡核基因组草图与其线粒体基因组比对发现红原鸡核基因组中存在3段较长的线粒体假基因，利用PCR方法扩增鸡形目10种鸟类和雁形目中鸳鸯核基因组中3段numt序列：cyt b numt、ATP8及下游ATP6 numt和ND4+tRNA-His+tRNA-Ser numt，测序后分别比较线粒体基因与核同源numt序列的进化特点和进化速率，确定numt序列插入核基因组的时间，以及结合GenBank已登录的日本鹌鹑、蓝胸鹑、黑鹇、蓝鹇和1个雁形目物种——白鹅雁(A.albifrons)相应序列探讨两种同源序列对于鸟类系统进化发育重建的重要作用。

According to the results of Blastn and Blastp in GenBank, it shares 72%,70%,60%,54% nucleotide sequence identity and 79%,73%,65%,57% amino acid sequence identity with Hordeum vulgare lipid transfer protein gene LTP7a2b and LTPcw-19, Zea mays ltp and Brassica napus ltp separately.

将核苷酸序列和推断的氨基酸序列在GenBank中进行比较，发现Siltp与大麦脂转移蛋白LTP7a2b和LTPcw-19，玉米脂转移蛋白，欧洲油菜的脂转移蛋白基因的核苷酸序列的同源性分别为72%，70%，60%，54%；氨基酸序列的同源性分别为79%，73%，65%，57%。

ConclusionComparisons of SD07LK1 sequence with that of the other Avian leukosis viruse strains, by using DNAstar software, demonstrated that the genes gag and pol of ALV-J were relatively conservative, the nucleotide identity of all the strains was over 95.0%. However, the gene env identity was only in the ranges between 88.6 and 94.0%.

将该序列与另外已完成的全基因组序列的比较表明，ALV-J的整个基因组gag和pol基因相对保守，各毒株间对应基因的同源性分别在95.0%以上，env基因的同源性仅为

This first comprehensive analysis includes genes and proteins and their relation to human disease, repeated sequences, comparative genome-wide studies of mammalian orthologous chromosomal regions and rearrangement breakpoints, reconstruction of ancestral karyotypes and the events leading to existing species, rates of variation, and lineage-specific and lineage-independent evolutionary events such as expansion of gene families, orthology relations and protein evolution.

这次全面分析包含了三种动物的基因组序列和蛋白产物以及它们与人类疾病相关性分析，重复序列，哺乳动物同源染色体区域和重排断点的比较基因组研究，祖先核型的重建，产生现有种群的事件，变异率以及种系特异和不依赖种系的事件如基因家族扩展，同源关系以及蛋白进化。人类19号染色体序列被破译了。

This first comprehensive analysis includes genes and proteins and their relation to human disease, repeated sequences, comparative genome-wide studies of mammalian orthologous chromosomal regions and rearrangement breakpoints, reconstruction of ancestral karyotypes and the events leading to existing species, rates of variation, and lineage-specific and lineage-independent evolutionary events such as expansion of gene families, orthology relations and protein evolution.

这次全面分析包含了三种动物的基因组序列和蛋白产物以及它们与人类疾病相关性分析，重复序列，哺乳动物同源染色体区域和重排断点的比较基因组研究，祖先核型的重建，产生现有种群的事件，变异率以及种系特异和不依赖种系的事件如基因家族扩展，同源关系以及蛋白进化。生物谷评论：人类19号染色体序列被破译了。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！