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These results indicated some differences from other investigated autopolyploid plants in autopolyploid watermelon. Autotriploid watermelon, in particular, showed unusual characters of genome structure and DNA methylation status, which may be closely associated with the oddploidy effect and the excellent traits present in triploid plants.

综上结果证明,西瓜同源多倍化过程中发生的遗传和表观遗传改变与已报导的一些植物的同源多倍化有所不同,特别是三倍体西瓜,其表现出独特的基因组结构及DNA甲基化状态变化特徵,而这可能与植物的奇倍性效应及三倍体西瓜优良性状的形成密切相关。

The sequence analyzing revealed that a sequence with a total length of 861 bp including 842 bp nucleotides of ATPase8 ATPase6 gene and partial sequence of the mitochondria CO Ⅱ was cloned. The ATPase8 and ATPase6 genes of Columba livia were proved to have good homology(88.1%~75.0%) with the other 5 species of birds recorded in the GenBank and it has 88.1% and 86.5% homology with Streptopelia orientalis.

结果表明:克隆得到了家鸽ATPase8-ATPase6基因842 bp及COII的部分序列共861 bp,用DNA分析软件对家鸽ATPase8和ATPase6基因与Genbank中的5种鸟类的ATPase8和ATPase6基因序列进行比较分析,表明家鸽与其他5种鸟类的ATPase8和ATPase6基因具有较高的同源性(88.1%~75.0%),其中与山斑鸡的同源性最高,分别为88.1%和86.5%。

In the prediction of protein structural classes, the residue order along the sequences takes an important role; while as in the prediction of protein subcellular locations, the amino acid composition is the most important factor.

在蛋白质的亚细胞定位预测中,氨基酸组成仍然是最重要的特征,蛋白质的亚细胞位置与序列同源性的关系没有结构与序列同源性的关系强。

These observations may provide a vaccine strategy for cancer therapy through the induction of the autoimmunity against the molecules associated with tumor growth in a cross-reaction with a single xenogeneic homologous gene and may be of importance in the further exploration of the applications of other xenogeneic homologous genes identified in human and other animal genome sequence projects in cancer therapy.

这些结果提示,通过单个异种同源基因以交叉免疫的方式打破肿瘤生长相关分子的自体免疫耐受是一种新的肿瘤疫苗治疗途径,并显示出进一步探索人类和其他种属的异种同源基因在肿瘤免疫治疗应用中的重要性。

We obtained a 1.5Kb DNA fragment of the succinate dehydrogenase flavoprotein subunit by PCR amplification and carried out the homolog analysis with those of other organisms.The result indicated a high homologh in this fragment among the five organisms.

通过PCR方法得到了琥珀酸脱氢酶黄素蛋白基因的一个1.5KB大小的DNA片段,并与其它生物的同种酶的基因序列进行了同源性分析,发现在所比较的五种不同生物之间这个区段具有很高的同源性。

RESULTS suc2 gene fragment of about 1.5 kb was amplified and cloned. DNA sequencing confirmed that , compared with the published sequence, the cloned suc2 gene had correct sequ ence except for one point mutation, but that did not alter the amino acid sequence o f t he encoded peptide. By transformation of yeast cells with a constructed gene dis ruption vector, four yeast clones were obtained which showed correct auxotrophy. PCR and DNA sequencing indicated that these yeast clones had the expected genot ype.

结果用 PCR从酵母基因组DNA中扩增出大小约1.5 kb的suc2基因片段,序列测定表明除585位有一点突变外,所得suc2基因与文献报道相同,构建的定位突变载体导入酵母细胞后,得到4株营养型符合的突变体,PCR表明这些突变体中均有suc2基因的同源重组,取其中1株的PCR产物进行序列测定证实了同源重组的发生。

Based on the biochemical analysis of the two proteins in vitro, the results of yeast two-hybrid experiment and protein-interaction in both wild type cells and C48 strain, we conclude HetR can form a homodimer through the Cys48 residue. We also show that the dimerization is required for heterocyst differentiation.

基于体外两种蛋白的性质比较和酵母双杂交的结果,并结合相应蓝细菌野生型和突变株C48A中HetR蛋白性质的体内研究表明:HetR蛋白可以通过第48位半胱氨酸残基形成共价结合的同源二聚体,该同源二聚体是异型胞正常分化所必需的。

For example, we have conducted the sequence screening on the binding sites of the interface of Barnase/Barstar complex and found that our DGA method can not only determine which position is more conservative and more important, but also redesign the binding sites rationally. The random sequence design for α,β,α/β and α+β four folding motifs have also been practiced and revealed that there are both homology evolution and heterology evolution of protein sequences compatible the same backbone motif.

例如,通过对典型的Barnase/Barstar复合物界面研究发现,它可以确定界面结合位点的重要性和保守性,并对结合位点进行合理的序列重新设计;通过对α、β、α/β、α+β等四种折叠模式骨架的随机序列设计,发现在蛋白质进化过程中,对同一种结构模式同时存在序列的同源进化和非同源进化这两种趋势。

Homology analysis showed that the sequence of GoCV-yk01 had 91%-93 % similarity to that of Taiwan and Germany strains.

基因组序列分析表明,浙江永康株GoCV-yk01全长1821bp,具有圆环病毒共同的与病毒复制相关的茎环结构和Rep蛋白保守基序等特征,它与德国、中国台湾发表的序列在全基因组水平有91%~93%的同源性,在Rep和外壳蛋白的氨基酸水平有94%~97%的同源性。

Comparisons of the ASPM homologue proteins show high conservations among the species and are therefore proposed to share biochemical functions.

比较各物种生物间的ASPM同源基因,可发现其蛋白质结构带有很高度的保留性,也因此各物种间的ASPM同源基因可能有相同的生物功能。

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