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At home and abroad published the same 24 NDV sequence homology analysis showed that the nucleotide sequence homology of 80%-98.4%, and amino acid homology of 87.2%-98.2% in between.

与国内外发表的24株 NDV 相同序列的同源性分析表明,其核苷酸序列的同源性在80%-98.4%之间,氨基酸同源性在87.2%-98.2%之间。

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术,对比研究正常与白化大菱鲆有眼侧皮肤组织,克隆差异表达基因,经测序,RT-PCR 验证,差异表达基因片段DEN-1(GenBank登录号:DQ886390)与DEN-2(GenBank登录号:DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达;通过BLAST检索发现,DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖:糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖:神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性;DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

The results showed that Me-c84 shared 98% similarities with the 26S ribosomal RNA gene from Sarracenia purpurea, while M14-86 shared97% similarities with the 26S ribosomal RNA gene from Portulaca Grandiflora.

将已经获得的两条cDNA序列与NCBI网站上的GenBank数据库进行同源序列比对,结果显示Me-c84核苷酸序列与瓶子草等植物的26S核糖体RNA部分基因同源性达到98%。M14-86核苷酸序列与大花马齿苋等植物的26S核糖体RNA部分基因同源性达到97%。

OPY13-661 sequence has identity with 16 EST sequences from European grapes, and shows 89% identity with 2 EST sequences from abiotic stressed leaves of Vitis vinifera var. Chardonnay.

OPY13-661序列与欧洲葡萄16条EST序列有同源性,其中与"赤霞珠"cDNA克隆的EST有较高的同源性,与"霞多丽"的2条叶片非生物胁迫有关的EST序列同源性均为89%。

By blast in GenBank, clone Y1218 shared 72% homology with Chromobacterium violaceum, clone Y1814 shared 88% homology with Pseudomonas putida,clone Y0140, 80% with Bacillus sp., and clone Y1038, 71% with Pseudomonas fluorescens. Some clones had homology with the degreadation of aniline, methyl parathion microbial organisms.

有80%的同源性,Y1038克隆和荧光假单孢有71%的同源性,有部分克隆和一些具有降解苯胺、甲基对硫磷等有机物的微生物有同源性。

Comparabilityof some segments reached about 86% to 90% including Arabidopsis thaliana CIPK7、Arabidopsis thaliana At3g23000 gene、Arabidopsis thaliana SNF1 related protein kinase(At3g23000; MXC7.3) mRNA、Cucumis sativus RBL1 mRNA, complete cds、Ranunculusmacranthus ribosomal protein L14 (rp114) gene, partial cds、Daucus carota chromasomecomplete genome and so on. There are also some other segments had a low identity andconsidered as unknown genes.

对部分差异条带进行了克隆测序,登陆GENEBANK对差异cDNA片断的序列进行核苷酸及氨基酸的同源性对比,其中一些片断与拟南芥CIPK7、拟南芥At3g23000基因、拟南芥SNF1相关蛋白激酶mRNA、栽培黄瓜RBL1mRNA全序列、毛茛核糖体蛋白L14基因、胡萝卜染色体基因组等均有86%-90%左右的同源性,还有部分片断的同源性很低,可能为未知基因序列。

Eight differential expression bands were cloned and sequenced.Blastn analysis showed that seven of them had highly homology(81%~100%)with EST sequences of Triticum aestivum and Hordeum vulgare.Six of the seven sequences came from Triticum aestivum,and one sequence came from Hordeum vulgare.

对上述5种特异表达类型中8条特异表达片段进行序列测定和分析,在相应的数据库,经Blastn比对,有7个序列在数据库中找到同源序列,且与普通小麦和大麦中的EST序列有较高的同源性,同源性为81%~100%,其中有6个来自普通小麦,1个来自大麦。

Methods : Level of nm23 - M2 expression of the endometria from day 2 , day 5 and day 7 pregnancies were carried out by RT - PCR and immunohistochemistry.

小鼠与人类nm23基因具有高度同源性,其两亚型分别为nm23一Ml(与人的nm23一Hl 的同源性为95%)和nm23一M2与人的nm23一HZ同源性则高达98

The four experimental virus strains and the three collate virus strains were divided into two groups, and belonged two evolutionary branches. Although ADV-DL1, DL2 and DL3 were all belonged to the Da Lian separated strains, they had the ulterior genetic relationship. The nuclear sequences of the obtained experimental ADV virus strains, ADV virus collate virus strains and typify virus strains of other four Parvovirus generic were cladogram analyzed with the Clustal W method of software DNA Star.

结果表明,ADV-Utah与四个实验毒株的序列同源性较高,同源率为92.9-93.4%,ADV-G、ADV-SL3与实验毒株的同源性较低;四个实验毒株和三个参考毒株被分别划归为两个组,分属于两个进化分支;实验毒株中ADV-DL1与DL2和DL3虽然同为大连分离株,却表现出较远的亲缘关系。

The yeast Pxa1p and Pxa2p dimerize to form a functional transporter involved in very long chain fatty acid oxidation in the peroxisome. The formation of PMP70 assembles as dimeric or oligomeric forms on peroxisomal membranes implies that Pxa2p may form a homodimers.

同源於Pxa2p的PMP70被证实会形成同源二聚体或多聚体,这对於Pxa2p是否也会形成同源二聚体或多聚体的研究,是非常有意义的。

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