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Result showed that dorking CSF gene was 435 bp in size with an ORF encoding 141 amino acids residues, the nucleotide homology between dorking CSF and CSF gene of Gallus, human, canis, Bos taurus, Sus scrofa, sheep, mice was respectively 98.4%%, 28.7%, 29.2%, 33.6%, 30.8%, 29.4% and 34.7%, Amino acids homology was respectively 95.2%, 8.3%, 9.0%, 15.3%, 12.4%, 11.0% and 12.0%.

序列分析表明,CSF的cDNA开放阅读框为435 bp,编码144个氨基酸,与红原锦鸡的序列比较,其核苷酸的同源性为98.4%,氨基酸的同源性为95.2%,同人、犬、印度野牛、野猪、绵羊和老鼠的CSF序列作比较,其核苷酸的同源性分别为28.7%,29.2%,33.6%,30.8%,29.4%和34.7%,氨基酸的同源性分别为8.3%,9.0%,15.3%,12.4%,11.0%和12.0%。

We retrieve the amino acid sequence by BLAST software in NCBI server to compare the homology and found that in GenBank+EMBL+DDBJ+PDB there are 100 sequence have the homology with this sequence, these 100 sequence are all sequence of PI. Among this the Amaranthus hypochondriacus have the maximum homology, then the Cucurbita, Arabidopsis thaliana, Linum, Euphorbiaceae, etc.

将BPI-2基因所推导的氨基酸序列在NCBI服务器上用BLAST搜索软件进行同源性基因检索,结果在GenBank+EMBL+DDBJ+PDB数据库中显示100条有同源性的序列,这些序列均为蛋白酶抑制剂序列,其中同源性较高的依次是苋属、南瓜属、拟南芥、亚麻属、大戟属等,相应氨基酸序列的同源性在分别为50%~63%之间。

Lines which were 2n=21"+1" and one line which was 2n=20"+2", one line which was 2n=43. The salt-resistance addition line Line15 was confirmed as a Disomic alien addition line by C-banding, SSR, FISH methods, and the two alien addition chromosomes had close correlation with the long arm of the second homoeologous group, the centromere region of the third homoeologous group and the short arm of the seventh homoeologous group. We also confirmed the particular C-banding type of the alien addition chromosomes by C-banding following FISH method. The drought-resistance addition line Line17 was found also as a Disomic alien addition line by SSR and FISH detection. The alien addition chromosomes had close correlation with the short arm of the second homoeologous group.

对导入赖草优质耐盐基因的株系Line15进行了C-分带、荧光原位杂交、SSR相结合的鉴定,确认Line15是一个二体异附加系,附加的一对染色体与小麦的第二同源群长臂、第三同源群着丝粒和第七同源群短臂密切相关,并确定了Line15的外源染色体C-分带特征带型,为目后鉴定Line15附加系的真伪提供了简便快捷的途径;对抗旱附加系Line17进行了荧光原位杂交和SSR检测,得出Line17也是一个二体异附加系,外源染色体与小麦的第四同源群短臂密切相关。

The homology analysis showed that these 5 fragments had very high homologies with the maize mRNA that hade been separated . S_ had 80% homology with cytochrome b5 gene of N.tabacum . SCT-TG had over 90% homology with α-tublin gene of Z.mays , Eleusine indica, Hordeum vulgare, Oryza sativa and Setaria viridis.

序列同源性分析表明:这 5 条片段都与已经分离的玉米 mRNA 有很高的同源性,S_与烟草细胞色素 b5基因有 80%的同源性、SCT-TG与玉米、牛筋草、大麦、狗尾草、水稻等的α-微管蛋白基因有 90%以上的同源性。

Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

利用蛋白质结构同源建模工具,分别以人丁酰胆碱酯酶(1p0i:A)和果蝇乙酰胆碱酯酶(1d×4:A)的蛋白晶体结构为模板,对嗜虫书虱2个AChE的三维结构进行同源建模,并在三维结构中发现了AChE的酶解活性位点,证明嗜虫书虱体内也存在2个AChE基因。2.2嗜虫书虱β-actin基因克隆及乙酰胆碱酯酶基因mRNA表达水平研究目前关于嗜虫书虱的分子生物学研究较少,在GenBank中没有可用作内参基因的序列,因此本研究从嗜虫书虱体内克隆获得β—actin基因片段(GenBank登录号:FJ041117),该片段长度为822 bp,编码273个氨基酸残基,同源性比对分析表明该片段与其它昆虫的β—actin基因具有很高的同源性。

The result of BLASTx showed that Maasr1 was homologous with Oryza sativa abscisic acid-inducible and stress-inducible protein (Asr1) mRNA (86%),Saccharum officinarum SoDip22 mRNA for drought inducible 22 kD protein (84%),Lilium longiflorum anth (A23) mRNA (86%), and Prunus armeniaca abscisic stress ripening protein homolog mRNA(82%).

用Maasr1基因作BLASTn比对发现它可能与水稻ABA和胁迫诱导蛋白(ASR1)的mRNA有86%的同源,与甘蔗干旱胁迫蛋白(SoDip22)的mRNA有84%的同源,与百合花A23基因的mRNA有86%的同源,与野黑杏脱落胁迫成熟蛋白的mRNA有82%的同源。

The open reading frame of cDNA is of 603 bp, which encodes GH precursor consisted of 200 amino acid residues. Sequence alignment indicates that the average value of sequence homology approaches to 92.4% among 7 species of siluriforms catfish, the highest is 95.8% between Silurus, meridicnalis and S. asotus, lowest is 89.4% between C. lazera and S. asotus.

克隆的革胡子鲶GH cDNA开放阅读框全长603bp,编码由200个氨基酸残基组成的生长激素前体。5个科7种鲶形目鱼类GH基因序列同源比较结果表明,7种鲶形目鱼类间GH基因序列的同源性很高,平均为92.4%,大口鲶和鲶之间的同源性最高,为98.5%,革胡子鲶和鲶之间的同源性最低,为89.4%。

Sequence analysis indicated that Arabidopsis AGL15 and GmAGL15 shared 61% of identity at amino acid level, especially 84.3% in the MADS domains, 63.2% in the K domains.

以同源序列法克隆了大豆MADS-box基因GmAGL15,序列分析表明,该基因编码的氨基酸与拟南芥AGL15蛋白的同源性为61%,其中在MADS区的同源性为84.3%,K区的同源性为63.2%。

Compared with other potyviruses, there is the highest identity between WMV-CHN with Soybean mosaic virus, the nucleotide and amino acid identity is 78.4% and 86.1%, respectively; the second are Peanut stripw virus, Bean common mosaic necrosis virus, Bean common mosaic viurs, Blackeye cowpea mosaic viurs and Zucchini yellow mosaic virus, the homologies of nucleotide and amino acid are 68.3% and 70.9%, 67.2% and 71.2%, 67.3% and 70.3%, 67.7% and 70.9%, 62.2% and 62.9% in turn, respectively. The 5′-termini of WMV is intimately correlated with evolution of viruses.

与其他Potyvirus属成员相比,同源性最高的是大豆花叶病毒,核苷酸和氨基酸同源性分别为78.4%和86.1%,其他依次是花生条纹病毒病毒、菜豆普通花叶坏死病毒、菜豆普通花叶病毒、黑眼豇豆花叶病毒和小西葫芦黄花叶病毒,核苷酸和氨基酸同源性分别为68.3%和70.9%,67.2%和71.2%,67.3%和70.3%,67.7%和70.9%,62.2%和62.9%。

The nearly full-length CP sequence of 308 aa of SCMV-NCH was compared with the counterpart of SCMV, Sorghum mosaic virus, Maize dwarf mosaic virus; Johnsongrass mosaic virus, Zea mosaic virus and Pennisetum mosaic virus, the six virus species in SCMV subgroup in Potyvirus, respectively. A high level of sequence homology (86.2%) was observed between SCMV-NCH and SCMV-A, while only 56.4% to 72.2% sequence homology was found between SCMV-NCH and the other five virus species.

与SCMV亚组SCMV、高粱花叶病毒、玉米矮花叶病毒、约翰逊草花叶病毒、玉米花叶病毒、白草花叶病毒等6种病毒代表性株系外壳蛋白氨基酸序列同源性比较结果显示,SCMV-NCH与SCMV的同源性最高(86.2%),与其他5种病毒同源性相对较低(56.4%~72.2%)。

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