同源物

The key research progresses and results that we accessed by the project were as follows: 1 A pair of degenerated primers were designed in the conserved domain which based on the amino acid sequence of HAK in Arabidopsis thaliana. The 500 bp fragment of HAK were amplified from the roots of Puccinellia tenuiflora by RT-PCR. The full sequence of HAK were obtained by 5' and 3' RACE methods and were determined and analyzed.

本项目主要取得了以下研究进展和成果：（1）根据已有HAK转运蛋白氨基酸序列在保守区域设计简并PCR引物，用反转录PCR方法，扩增出500bp的小花碱茅根HAK片段，通过5''和3''RACE的方法获取了该HAK的全长序列，完成了测序和序列同源性分析。

Because homeotic gene mutation and floral organ variation is a direct relationship,therefore the mutant application is the current functional genomics study developmentdirection. We discovered one mutant ah from line 458, which was a line ofthe twin-seedling strain W2555.ln this study, we have investigated the morphogenesisprogress, genetic analysis of mutant traits and molecular tagging of related gene.

目前，由于在水稻中发现的花器官突变体很少，所以对水稻花发育的研究主要是利用双子叶植物MAS-box基因的保守序列设计引物，在水稻基因组或者cDNA文库中筛选与双子叶植物花器官发育相对应的MADS基因，因为同源异型基因的突变与花器官的变异是直接对应的关系，所以突变体的应用是当前功能基因组学的发展方向。

Methods Granulocyte/macrophage colony stimulating factors and interleukin 4 were used to cultivate DC from peripheral blood of hepatocellular carcinoma patients.Tumor associated antigen extracted from human liver cancer HepG2 cell line was used to activate the DC,which will initiate homogenic T lymphocyte to form cytotoxic T lymphocyte.HepG2 cells were transplanted to the nude mice and were treated with CTL.Expression of PCNA in tumor cells was evaluated.

联合应用粒/巨细胞集落刺激因子及白介素-4(IL-4)直接从肝癌患者外周血中培养出DC；以源于人肝癌细胞系HepG2细胞的肿瘤抗原粗提物刺激DC；DC激活同源的T淋巴细胞产生细胞毒性T淋巴细胞；建立人肝癌细胞系HepG2裸鼠移植瘤模型；CTL治疗人肝癌细胞系HepG2裸鼠移植瘤；检测移植瘤标本肿瘤细胞PCNA表达水平。

These results demonstrated that BmCPV,DsCPV and LdCPV were homologous as classfied in the same electropheres type I,and that this RT\|PCR assay could be used for early,rapid,sensitive and specific detection of DsPCV infection in the natural population of the pine moth,due to the apparent different feeding habits of Bombyx mori,Dendrolimus ssp.and Lymantria dispar larva each other and the no infection of BmCPV and LdCPV to Dendrolimus ssp.

由于松毛虫与家蚕、舞毒蛾相互之间食性不同，而且BmCPV和LdCPV对松毛虫无感染性，即在松毛虫体内不会有BmCPV病毒和LdCPV病毒的感染，因此该结果一方面从分子水平上证实了DsCPV与BmCPV、LdCPV存在基因同源性，同时也表明了依据BmCPV的基因序列设计引物对DsCPV基因组核酸建立的RT-PCR扩增体系，可以作为松毛虫种群中DsCPV的一种敏感、特异、早期、快速的检测手段。

The Sry gene sequence of Muntiacus crinifrons was acquired and the conclution was that there are 83% homology between the human and Muntiacus crinifrons. It was testified that in all mammal Sry gene is consertive. On the other side the Sry gene was located to the Y2 chromosome of the Muntiacus crinifrons.

最后提取雄性黑麂的基因组DNA，并用同一对引物对其进行扩增，亦得到Sry基因的片段，对此扩增片段进行克隆，测序，结果表明其与Y2染色体得到的Sry基因片段完全一样，与人SRY基因的同源性均为83%。

The genetic identity was determined by the identical numbers and location of bands showed in products of repetitive extragenic palindromic se...

利用重复片段引物PCR的基因分型方法，根据条带数目和位置完全相同为同一基因型的分型方法[1]判定是否存在基因同源性。

The genetic identity was determined by the identical numbers and location of bands showed in products of repetitive extragenic palindromic sequence-based PCR ..

利用重复片段引物PCR的基因分型方法，根据条带数目和位置完全相同为同一基因型的分型方法[1]判定是否存在基因同源性。

The results of sequencing was contrasted by BLAST software. The sequence of the PCR products shared 100% homology with Pasteurella multocida reference strain.

设计1对引物进行PCR扩增、克隆之后，送往大连宝生物公司测序，测序结果运用BLAST软件比对，与多杀性巴氏杆菌同源性为100%。

The phylogenic tree of LYSV was clustered into four groups.

LYSV不同分离物间CP氨基酸序列存在明显差异，同源性在75.8%～100%之间。

The identification of protein were performed by searching the mass spectrometry data from the NCBInr database using the software of Sequest Bioworks3. 2. The results indicated that over 25 protein components including acid and basis phospholipase A2, nerve growth factor, presynaptic neurotoxin, hemorrhagic factor, thrombin-like enzyme, serine protease, glycogen synthase, metalloproteinase, maturase, L-amino acid oxidase, plasminogen activator, adhesion, cysteine-rich secretory protein, disintegrin, fibronectin-binding protein, fibrinogen clotting inhibitor A chain, vascular apoptosis-inducing protein 1, pyranose 2-oxidase, carbamoyl-phosphate synthase, as well as the homology proteins of snake venom ablomin, ablomin, catrin, mucrofirase, pallabin, salmosin and triflin homologues were identified from the GSSV.

从GSSV中鉴定出超过25种蛋白组分，主要包括：酸性磷脂酶A1、碱性磷脂酶A2、神经生长因子、突触前神经毒素、出血因子、类凝血酶、丝氨酸蛋白酶、糖原合酶、金属蛋白酶、成熟酶、L-氨基酸氧化酶、纤溶酶源激活物、外源凝集素、富含半胱氨酸分泌酶、去整合素、纤连蛋白结合蛋白、纤维蛋白原凝固抑制因子A、血管凋亡诱导蛋白1、氨甲酰基磷酸合成酶、吡喃糖－2-氧化酶以及ablomin， catrin， mucrofirase， pallabin， salmosin和triflin的同源蛋白。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。