同时的

An excessive concentration of power in the Kremlin that has wiped out regional autonomy and fostered corruption; The so-called peace in Chechnya has been won by handing control of the republic to a former rebel, Ramzan Kadyrov; the competitiveness of the non-oil economy has eroded sharped since the devaluation of 1998; The concept of manufactory for export scarcely exist, outside the arms industry; The long-dram-out Yukos affairs, which began in mid 2003 presaged not only an assault on property rights and the rule of law and also a resurgence of state control over, and interference in the economy.

第一，克里姆林宫的高度集权剥夺了地区的自主权同时也滋生了腐败问题；第二，所谓的车臣和平实际上是把共和执政权交给了昔日的车臣叛军首领卡狄羅夫；第三，俄国这个无油国家的竞争力已经从98年的货币贬值开始踱步下降；第四，除了武器出口，基本上没有制造业的出口；第五，从2003年中年开始的尤科斯事件，不仅预示了这是对个人所有权的侵害，而且还是对国家法律和管理权利产生了负面影响，同时也干涉了经济。

I have my difinately objuective that is to study overseas, bring there the chinese culture and voice, it's really need rational on boycotting, u slanging by chinese language, and make some jerk joke about foreigners, it's absolutely no concern, and when u slang the comments also include our friendly foreigners who plunk for china! hence, we really need rational, to learn a foreigner language, representing our real china, that what we should and could do. for boycotting carryfour, all i can say,supporting our own enterprises, i just cannot over my own block which let me feel that it is the market who insults chinese keeping, though i go for shopping there every week before, and it's really cheap...

我现在目标很明确，就是出国去学习西方的文明，并且把中国的东西带过去，抵制确实需要理智，你用中文骂了人家半天，说些骂外国人的恶心笑话，这有什么意义，你在骂的同时，也同时骂了那些原本对我们是有好的外国人，他们在帮我们~所以，一定要理智，学好一门外语，把真正的中国传达给世界，这才是我们要做的，至于抵制家乐福，这个，我只能说，支持民族自主产业，我自己是憋不过自己，感觉法国人都这样侮辱了自己，我是拉不下脸来再去那购物，虽然我以前每周都去，那的东西确实便宜。。。

This research method is to use the three-dimensional mapping software to draw the cardiovascular stent of different size first , next is it create their cardiovascular stent boundary conditions and simulation their of support after implantation according to finite element method into the human body to go; Receive different residue pressures and pressure of the repeated blood circulation , greatest stress , meets an emergency and the relation between total deformation amount caused; Being imitated the cardiovascular stent , the position with damage of fatigue life in the blood vessel by its greatest stress value at the same time ; But by the simulation result of the above , can understand that use life of Palmaz-schatz cardiovascular stent has been more than 109 up till now ; At the same time , can know the position where the stress two times concentrates , the right angle position joined in the rib and rib of Palmaz-schatz cardiovascular stent too.

本研究的方法乃是先会使用三维绘图软体画出不同尺寸的血管支架，然后在根据有限元素法去设定其血管支架的边界条件和模拟其植入人体后；受到不同的残留压力与反覆血液流动的压力，所造成的最大应力、应变及总变形量之间的关系；同时在由其最大应力值去模拟血管支架，在血管中的疲劳寿命与疲劳损坏位置；然而由以上的模拟结果，可以了解到目前Palmaz-schatz血管支架之使用寿命为109以上；同时也可以知道二次应力集中的位置，在Palmaz-schatz血管支架之肋和肋连接的直角位置。

Meanwhile, probability theory made progress and became matured. During 1921-1928 Borel considered dealing with some strategic game problems with probability theory, form which he established several useful conceptions in Game theory, and it was at that time that Game theory was found in the rudiment.

同时随着概率理论的进一步发展和成熟，波莱尔在1921-1928年首先用已有的概率理论考虑了几个策略博弈的例子，并创造出了博弈理论中几个非常有用的概念，同时产生出了现代博弈理论的思想萌芽，在这一时期对博弈论的早期理论有贡献的还有思特豪斯、波莱尔的学生威利和他的同事。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

Second is studying the traumatic condition changes in a choosey pressure at different post-PT phases. Moreover, we detected model PLA_2 activity, express of PLA_2 subtype mRNA by fluorescent quantitative RT-PCR technique, protein express and site by Western blot and IHC at different post-PT phases. At last, we studied the PLA_2 inhibited effects and model protection of Variabilin in vivo.

研究在不同压强的外力作用下，模型指定时相点的一般情况和存活率、大体、光镜及电镜下胰腺组织的病理变化和常规酶学变化，得到稳定建模致伤压强；然后，固定致伤压强，研究胰腺创伤后不同时相点的伤情变化(包括一般情况、胰腺组织的病理变化、AMS和LPS活性变化、血常规、血生化、胰腺细胞凋亡以及胰腺组织细胞生长周期变化情况)；再次，采用3H-花生四烯酸标记大肠杆菌膜作为底物的方法检测大鼠胰腺创伤后不同时相点的PLA_2活性，利用荧光定量RT-PCR技术检测其亚型mRNA的表达，利用Western blot和IHC技术检测其蛋白表达量和蛋白组织定位；最后，将Variabilin体内应用于急性胰腺创伤大鼠模型，研究其对模型的保护作用和对PLA_2的抑制效应。

By hand tiling the whole piece in Froot Loops, adding a couple of accent pillows in Captain Crunch and perhaps a neutral Cornflake pillow or two for some balance, the interest and textural level would've risen substantially.

在底部铺上环形状的手工艺瓷砖，同时增加两个情侣的抱枕靠垫，也可以是一个中立性色彩的抱枕或是两个相同的可以保持平衡性的抱枕，这样的设计会同时充分的提高趣味和组织构造上的水平。

The version itself, which accords pretty closely with the translation in Tyndale's New Testament, no doubt owed its general acceptance to an ordinance of 1541 according to which "his Grace perceiving now the great diversity of the translations (of the Pater noster etc.) hath willed them all to be taken up, and instead of them hath caused an uniform translation of the said Pater noster, Ave, Creed, etc. to be set forth, willing all his loving subjects to learn and use the same and straitly commanding all parsons, vicars and curates to read and teach the same to their parishioners".

该版本本身，它给予相当密切与翻译tyndale的新约圣经，毫无疑问，拖欠其普遍接受的一项条例的第1541根据该项规定，&他的恩典感知现在极大的多样性，翻译（属于父亲noster等），祂所意志，他们都将要采取的行动，而不是他们祂所造成一个统一翻译的说，父亲noster ，大道，信仰等，以中载明，愿意在他的所有爱好科目的学习及使用，同时并straitly指挥全部帕森斯， vicars和curates阅读和教导，同时向他们的教友&。

The version itself, which accords pretty closely with the translation in Tyndale's New Testament, no doubt owed its general acceptance to an ordinance of 1541 according to which "his Grace perceiving now the great diversity of the translations (of the Pater noster etc.) hath willed them all to be taken up, and instead of them hath caused an uniform translation of the said Pater noster, Ave, Creed, etc. to be set forth, willing all his loving subjects to learn and use the same and straitly commanding all parsons, vicars and curates to read and teach the same to their parishioners".

该版本本身，它给予相当密切与翻译tyndale的新约圣经，毫无疑问，拖欠其普遍接受的一项条例的第1541根据该项规定，&他的恩典感知现在极大的多样性，翻译（属於父亲noster等），祂所意志，他们都将要采取的行动，而不是他们祂所造成一个统一翻译的说，父亲noster ，大道，信仰等，以中载明，愿意在他的所有爱好科目的学习及使用，同时并straitly指挥全部帕森斯， vicars和curates阅读和教导，同时向他们的教友&。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。