同时发生

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

Cyrus Harding announced this result to his companions, and taking intoconsideration errors of observation, as he had done for the latitude, hebelieved he could positively affirm that the position of Lincoln Island wasbetween the thirty-fifth and the thirty-seventh parallel, and between thehundred and fiftieth and the hundred and fifty-fifth meridian to the westof the meridian of Greenwich.

赛勒斯·史密斯向伙伴们宣布了这个结果，同时，也象计算纬度时一样，估计了观察时可能发生的误差。他相信他可以肯定林肯岛的位置在纬度35度到40度之间，经度在格林威治子午线以西150到155度之间。

The leakage accidents in pipelines transportation not only cause great loss of material, but also are likely to cause corrosion leaks in the future at the same time. If we dont find the leak place in the pipelines in time, it easily occurs to the tragedy accidents of the fire, the blast and the environment pollution.

原油管道输送中的泄漏事故，不仅给企业造成巨大的经济损失，同时给管线内原油泄漏留下了隐患，如不及时发现，容易发生火灾，爆炸，环境污染等灾难性事故。

In fire place, all staff should absolutely listen to fire fighting responsible person. Commander in accordance with the actual situation of the scene, according to the order of succession, rapid evacuate not related personnel to a safe location, while the maximum capacity of organizations in the fire or to control the fire.

在发生火警或火灾的场所﹐所有人员应绝对服从该埸所防火责任人的统一指挥和命令﹐指挥员根据现埸的实际情况﹐按先后秩序﹐迅速撤离无关人员到安全地点﹐同时尽最大能力组织参与灭火或控制火势。

Adulterating fire-retarded inorganic compound into the asphalt will improve the fire-retarded performance of asphalt. Meanwhile, OGFC design method has been developed.

采用阻燃技术提高沥青混合料在发生火灾时的难燃性，同时提出OGFC的混合料设计方法。

Meanwhile, human brains turn out to be extremely permeable (they naturally mimic the neural firings of people around them).

同时，人类的大脑被证明是有浸透性的/相互联系的(他们自然的模仿神经元同周围的人发生联系)。

Both Grand Admirals were aboard their respective flagships, and both perished in the ensuing explosion.

两位元帅都登上了各自的旗舰，同时也都丧生于接下来所发生的爆炸。

But in a twist of fate among the practitioners of tooth-and-claw capitalism, it is the private equity buy-out groups which loaded with debt the companies they acquired and squeezed those assets to improve their returns that are now about to be bested by other masters of the fleet-footed financial arts.

然而，在弱肉强食资本主义的实践者中，命运发生了转折：如今，败在其他擅长快速金融艺术的大师手上的将是私人股本收购集团，它们让它们所收购的公司背上债务负担，同时为提高收益而拼命榨取那些资产。

Pigments potent and air temperature are are more important than other factors. The color changing of foliage trees leaf resulted from these comprehensive factors, We must fully respect them in landscape design. Meanwhile, we should make of the bionomics and ecologic habitus of these trees, and make sure to let them show vivid colors and the most beautiful landscape effictiveness in urban environment, ensure the landscape designs more scientific and artistic.

观叶树种的叶色是在许多因素综合作用下发生变化的，在利用观叶树种进行园林景观设计时必须充分考虑影响观叶树种叶色的各种因素，同时对观叶树种的生物学特性和生态习性有深入的了解，使其在城市环境中达到最鲜艳的颜色，产生最佳的景观效果，使园林植物造景更具科学性和艺术性。

The use of distiller's grain and corn stalks mixed feeding goats, simple operation, saving time and workers, do not mistake farming season, while reducing parasitic disease and the occurrence of Footrot, but also increase the amount of organic fertilizer storage.

利用酒糟与玉米秸混合饲喂山羊，操作简单，省时省工、不误农时，同时减少寄生虫病及腐蹄病的发生，还可增加有机肥储存量。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失