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Today's networks are all major banks in silver standard operating components, not only products of the same serious and product development cycles shorten, net silver in the individual business competitive at the same time, how in the enterprise networks business-level search for differentiation The key to winning a point.

今天的网银已经是各大银行的标准业务组件，不仅产品同质化严重，而且产品开发周期也大大缩短，在个人网银业务竞争激烈的同时，如何在企业网银业务层面寻找差异化的制胜点成了关键。

The activity of muscle Na+/K+-ATPase increased time coursely during the 8-day acclimation, but had not completely recovered within 8 days. This indicated that subadult Macrobrachium nipponense need a long time to adapt salinity changes.

3肌肉中的Na~+／K~+-ATPase活力在8天的适应期内逐渐升高，但仍未恢复到同温度对照组的水平，表明日本沼虾亚成虾对盐度的适应需要较长时间的调整。

Using poly (4-merhylpentene-l) as a membrane material and cyclohexane, irich-loroethylene, tetrachloroethylene, mixture of cyclohexane and trichloroethylene or tetrachloro-ethylene as solvents, the solution were cast to form homogeneous dense membranes, and the crystalline properties and surface morphology were investigated by DSC and TEM.

以聚4-甲基戊烯-1为膜材质，分别以环己烷、三氯乙烯、四氯乙烯、环己烷/三氯乙烯及环己烷/四氯乙烯为溶剂，研究了这些溶液浇铸膜的结晶性质和表面形态。结果表明，溶剂对PMP的相对溶解能力和挥发速度同所成膜内PMP的结晶形式和结晶度密切相关；不同溶剂体系的PMP溶液浇铸膜的表面形态也不尽相同。

The team, along with Alan Waggoner, professor of biological sciences and director of Carnegie Mellon's Molecular Biosensor Imaging Center, is now deeloping noel biosensors and fluorescent-based techniques to isualize stem cell differentiation in response to the bio-ink patterns.

目前，研究组同Carnegie Mellon大学的分子生物传感器成像中心的主任生物科学的教授Alan Waggoner一道，开发新型生物传感器和荧光技术以观察干细胞对生物墨水模式的反应情况。

Organozinc reagents can react with a variety of α,β-acetylenic ketones and aldehydes in the absence of any catalyst. The one-pot reaction affords β-branched Baylis-Hillman type adducts, the multifunctionalized alkenes, in excellent yields with high stereoselectivity.

这种顺式亲核加成反应的发生是由于该类有机锌试剂既具有较强的亲核性，又具有Lewis酸的特性，使有机锌中R基团从同位进攻中心碳原子而形成新的碳碳键。

The fluctuation of the system can be described as following:the fluctuation of main crop decreases from north to south due to rain,but the accumulated population overloading under nutrition criteria of calor...

在温饱型的热量营养标准下，人口的绝对和相对超载状况同土地生产力的波动性成正比，线性相关在 0 。83以上，空间分布规律与土地生产力的波动性一致。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Functional ASICs are usually believed to be tetrameric assemblies of homomeric or heteromeric subunits.However,recent Gouaux and colleagues crystal structure shows that there are three subunits in ASIC1 homomeric channel.

功能性的ASICs通常被认为是由相同或不同的亚单位形成的四聚体，然而最近Gouaux等的晶体结构研究表明ASIC1同聚体由三个亚单位装配而成。

PartⅡThe mechanism elucidation about effects ofα-(2,3)/(2,6)sialic acid on the Cx43gap-junction functions.(1) Westernblotting experiment showed that the decrease of sialic acid didn\'t changethe Cx43 expression and its phospholation level.(2) Westernblotting experiment showed sialidase didn,t change the ZO-1 expression,IP and confocal experiment showed sialidase improved the interaction of Cx43 andZO-1.(3) Westernblotting experiment showed sialidase didn\'t change N-cadherin expression,IP and confocal experiment showed sialidase promoted the complex formation ofCx43 and N-Cadherin.(4)Sialidase could increase the ERK1/2 phospholation level,and enchanedintercellular homotypic adhesion,Immunofluorometric assay showed sialidase couldpromote the N-cadherin cluster on the membrance.

第二部分：α—(2，3)／(2，6)唾液酸对肿瘤细胞CX43间隙连接功能影响的机制研究1、Westernblotting结果表明降低细胞膜表面唾液酸并不改变Cx43的表达及其磷酸化水平：但Cx43连接斑形成增多；2、Westernblotting结果表明唾液酸酶作用后细胞ZO—1表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与ZO-1的结合；3、Westernblotting结果表明唾液酸酶作用后细胞N-cadherin表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与N-Cadherin复合物的形成；4、唾液酸酶作用后细胞内ERK1／2磷酸化水平明显增加，细胞间同质粘附增加，以及免疫荧光表明唾液酸降低后可促进N-cadherin的膜成簇。

The variant most likely to show pI values different from that of HbA when run on an isoelectric focusing gel.

在跑IEF时最有可能表现出与HbA不同pI的变种。浪花带的成岩作用或渗滤作用是影响同生碇酸盐的起盐囱水变化的另一个因素。

This paper demonstrated the impact on the environment today, an important factor is Education.

本文主要展示了当今影响环境的一个重要因素——教育。

Article 16 The power to interpret these Procedures shall remain with the Ministry of Agriculture and CAA.

第十六条本程序由农业部、国家认证认可监督管理委员会负责解释。