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Objective To clone Horseradish peroxidase isozyme C3(HRPC3) in order to express the gene in vitro.

目的克隆辣根过氧化物酶同功酶C3(HRPC3)基因,为此基因的表达做准备。

aim to assess relationship between the increased lfa-1expression in intestine graft and acute rejection,and inhibitory effects of fk506on the expression of lfa-1.methods heterotopic small intestine transplantation was performed on inbred sd and wistar/a rats.the rats were randomly divided into4groups:control groups;isograft group(wistar/a→wistar/a);allograft group(sd→wistar/a);allograft plus fk506group(sd→wistar/a+fk506),

目的 探讨大鼠移植小肠组织内淋巴细胞相关抗原-1(lfa-1)的异常表达与急性排斥反应之间的关系,及fk506对其的抑制作用。方法选用近交系封闭群sd和wistar/a大鼠进行全小肠异位移植。实验分4组:非手术对照组;同基因移植组(wistar/a→wistar/a);异基因移植组(sd→wistar/a);异基因移植加用fk506治疗组[sd→wistar/a+fk506

The results show that both of relative rate and synonymous and non-synonymous coden substitution in eudicot clade are significantly higher than those in monocot clade, and the value of dN/ds uncovered possible positive selective pressure in eudicot clade.

结果表明:真双子叶植物分支中SQUA类基因的相对速率和同义与非同义置换位点均显著高于单子叶植物分支;同时,dN/dS值表明真双子叶植物分支中可能存在正选择压力。

Recently polypod ferns have been demonstrated to undergo diversifications in the Cretaceous after the rise of angiosperms. To further test this proposal, an adaptive evolutionary analysis was performed on the rbeL gene sequences of Polypodiaceous ferns under models allowing w (nonsynonymous/synonymous rate ratio) to vary among sites.

为验证蕨类植物在白垩纪适应被子植物兴起而发生分化的观点,本研究以水龙骨科附生蕨类为对象,利用位点间可变ω(非同义替换率d和同义替换率d的比值)模型对其rbcL基因的适应性进化进行分析。

The heterogenic C57BL/6J splenocytes disappeared at 1 day after transfusion, but the syngeneic BALB/c splenocytes could survive for a long time in the peripheral blood of BALB/c mice. The fluorescence intensity was not decreased in two kinds of splenocytes. The results demonstrated that CFSE-labeling with difference concentration is reliable, stable and convenient for spleen lymphocytes tracing in vivo.

异基因的C57BL/6J脾细胞在输注1 d后就快速消失,而同基因BALB/c脾细胞能够在BALB/c小鼠外周血中长期存在,但是两种细胞荧光强度均未见降低,说明荧光染料CFSE差异浓度标记活细胞进行体内示踪是一种稳定的示踪方法。

To elucidate the function of actin in translation we have studied 25 isogenic conditional yeast actin mutants.

为了阐明肌动蛋白在翻译中的功能,我们研究了25个同基因,同条件的酵母肌动蛋白突变体。

GM maize-fed rats were compared first to their respective isogenic or parental non-GM equivalent control groups.

GM喂食的老鼠,首先与它们各自的同基因或同父母非转基因等效对照组比较,随后比较六个参考组,它们用各种其它非转基因玉米品种喂养。

Anguillarum isolates, while 94.3% and 91.9% nucleotide identical to L. pelagius and Photobacterium damselae respectively. MP analysis showed that ayu-H080701 shared 97.6%-98.8 % amino acid sequence identical to L. anguillarum isolates, while lower than 75.6 % to other bacteria. Phylogenetic analysis showed that ayu-H080701 grouped constantly with L. anguillarum isolates. The biochemical, physiological tests and sequence analysis all strongly supported the identification of the pathogen causing ayu vibriosis in Ninghai country, China, as an isolate of L.

PCR扩增检测表明,细菌16S rRNA 基因通用引物和鳗利斯顿氏菌MP基因特异引物均能扩增到预期大小的特异性条带。ayu-H080701与鳗利斯顿氏菌16S rRNA基因核苷酸序列同源性最高,为99.4%~99.5%,与同属的海弧菌和美人鱼发光杆菌分别为94.3%和91.9%;ayu-H080701与鳗利斯顿氏菌MP氨基酸序列同源性高达97.6%~98.8 %,与其它弧菌科成员则低于75.6 %,系统进化树分析也揭示ayu-H080701与鳗利斯顿氏菌进化相关性最高。

Objective: To explore the effect of syngeneic bone marrow-derived mesenchyinal stem cells infusion in the induction of immune tolerance to murine skin allografts.

目的:探讨受体来源的同基因骨髓基质干细胞在促进异基因骨髓细胞诱导小鼠皮肤移植免疫耐受中的作用。

METHODS: Human AMSCs were isolated in vitro sterilely. At the third passage, 0.5 mL single cell suspension at 1×109/L was obtained and transplanted by tail venous pathway in transplantation group mice, Mice in the control group were injected with an equal volume of saline. APP-gene mice in the normal group were left intact. 5'-bromo-2-deoxyuridine labeled third-generation AMSCs expression was detected in mice brain tissue by immunohistochemical method.

无菌条件下体外分离培养人羊膜间充质干细胞,传至第3代将细胞浓度调整为1×109 L-1,经尾静脉注入0.5 mL至细胞移植组转APP+基因小鼠体内;对照组经尾静脉注入同体积的生理盐水;正常组转APP-基因小鼠不给予任何干预措施。

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在美国,慢性酒精中毒,肝炎是最常见的。

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