同基因的

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

Seek science and humane circulation, nomatter on the natural space, or in the field of the society, or is launchingomni-directionally during the process of studying , for example drink the ethicsproblem of the Human Genome Project to the security of the transgenic food, geneticengineering expert and ethicist have produced common attention , and the arrival ofcybertimes and prosperity of network culture, make the expert and art of the networktechnology learn to form alliances in the family again.

寻求科学与人文的融通，无论在自然的空间上，还是在社会的领域中，或是在研究的过程中，都全方位地展开着，比如对于转基因食物的安全性喝人类基因组计划的伦理问题，基因工程专家同伦理学家产生了共同的关注点，而网络时代的到来以及网络文化的繁荣，又使得网络技术的专家和艺术学家结成了联盟。

Further proceed intersection analysis and focus on children studies. The result indicates that experimental diagnosis based on RT-PCR, is homogeneity and the relation between norovirus and the symptoms of acute gastroenteritis is significant (OR, 2.7;95%CI, 2.2-3.2). Equally, the surveillance studies (OR, 2.5;95%CI, 2.1-3.0), outbreak studies(OR,4.7; 95%CI, 3.2-7.1), hospital-based studies(OR, 2.8; 95%CI, 2.0-4.1), and community-based studies(OR, 2.0; 95%CI, 1.2-3.3) have the same result as the former.

进一步将研究对象为孩童的文献进行交叉分层分析，结果发现，以孩童为研究对象的文献中，实验室诊断以基因体RNA为侦测标的的研究，其效果量具有同质性，且诺罗病毒与急性肠胃炎症状的相关性在此分层是显著的（OR=2.7，95%CI： 2.2-3.2），资料来源为监测性研究（OR=2.5，95%CI： 2.1-3.0）或疫情爆发研究（OR=4.7，95%CI： 3.2-7.1）以及研究场所为医院（OR=2.8，95%CI： 2.0-4.1）及社区（OR=2.0，95%CI： 1.2-3.3）的研究亦有相同结果。

In D3 gene with 5 site mutations. There exists individual variation in the COII gene, but this variation is less than that among other members of Myzomyia Series such as An. aconitus, An. jeyporiensis, etc. In addition, one haplotype different from both An. minimus A and C was found in these 3 genes studied.②The genetic distance between An. minimus A and An. minimus C is less than that among other members of Myzomyia Series. Molecular trees based on different genes and different methods show that An.

种系发生研究表明，微小按蚊复合体亲缘种A、C间的遗传距离远小于与之近缘的同系其它成员间的距离。2个分子标记3种方法构建的基因树显示，微小按蚊A、C的种系地位非常固定，不同地域来源、不同变异类型的微小按蚊始终在同一分"枝"上，与迈蚊系其它成员相比，呈现其相对稳固的单源性。

In this study, the cDNA fragments of G-L intergenic region and polymerase activity module in L gene of two street rabies virus were cloned and sequenced. The nucleotides sequence of these cDNA fragments mentioned above and their deduced amino acid sequence were compared with that of rabies virus strains, rabies-related virus strains and other Rliabdoviridae published previously. The result show that the homology of nucleotide sequence of G-L IGR among MRV, DRV is 83.3%, compared with other rabies-related virus strains, the homology of G-L IGR is between 58.3%-91.7%. the length of G-L IGR of rabies virus is different in Rhabdoviridae.

本实验对狂犬病病毒野毒株MRV、DRV的G-L间隔区以及L基因聚合酶活性部位进行了克隆和测序，将所测定的结果和推定的氨基酸序列与国内外公开发表的狂犬病病毒其它固定毒株、野毒株和同科不同属的病毒的相应部分进行了分析比较，结果表明MRV、DRV G-L IGR核苷酸序列的同源性为83.3％，与其它毒株的同源性在58.3％～91.7％之间；狂犬病毒G-L间隔区序列的长度在弹状病毒科不同病毒属之间有所不同。

The infection rate was significantly decreased if we upregulate Attacin expression by E.coli injection prior to trypanosome feeding.Attacin gene was cloned and expressed in Drosophilla S2 cells. Purified protein shows strong activity against E.coli, as well as trypanosome. But tsetse symbiont Sodalis, a gram negative bacterium, was quite resistant to Attacin.

克隆Attacin基因并在果蝇S2细胞中表达，对纯化的Attacin蛋白的研究表明它除了对大肠杆菌有很强的活性外，对锥虫也有较强的活性，而同为格兰氏阴性菌的采采蝇共生菌Sodalis则在试验中表现了较强的抗性。

In D3 gene with 5 site mutations. There exists individual variation in the COII gene, but this variation is less than that among other members of Myzomyia Series such as An. aconitus, An. jeyporiensis, etc. In addition, one haplotype different from both An. minimus A and C was found in these 3 genes studied.②The genetic distance between An. minimus A and An. minimus C is less than that among other members of Myzomyia Series. Molecular trees based on different genes and different methods show that An.

种系发生研究表明，微小按蚊复合体亲缘种A、C间的遗传距离远小于与之近缘的同系其它成员间的距离。2个分子标记3种方法构建的基因树显示，微小按蚊A、C的种系地位非常固定，不同地域来源、不同变异类型的微小按蚊始终在同一分&枝&上，与迈蚊系其它成员相比，呈现其相对稳固的单源性。

The FDA requires a full evaluation of a genetically engineered food crop when uncharacterized DNA sequences are used, or when food products may contain significantly altered nutrient levels, different compositions from substances currently found in foods, allergenic proteins, new antibiotic resistance markers, or levels of toxins significantly above those found naturally in edible varieties of the same species.

当使用不明特征DNA时或者当食物产品营养水平可能显著改变的、有不同于目前食品中组成成分的、有过敏原蛋白的、有新抗菌素抗体标记的或者毒素水平显著超出同物种可食用各品种中天然存在的这些情况出现时，食品药物管理局将要求完整评估一种基因工程处理过的粮食作物。

The company focusses on the research and development of new drugs in the treatment of pain, and early successes from the Grünenthal research include tramadol, currently world-wide the most prescribed weak opioid for the treatment of moderate to severe pain.

格兰泰的药品研究开发范围主要包括：镇痛药、抗生素、激素、治疗心血管疾病的基因工程药物、神经疾病药物等。-格兰泰集团的员工都有着强烈的自我责任感，我们细心准备，勇于探索，始终同公司一起共同发展

The results suggested heterogeneity in clinical phenotype. But more cases and further study in other exons were needed.

表明巨颌症临床表型的遗传异质性，同时尚需扩大样本量和进一步的实验研究探索新的突变位点和新的致病基因。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！