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Some of these genes have been successfully incorporated into plants . The insect-resistant transgenic plants inhibit development and decrease fecundity of homopteran insects , demonstrating practical potential of these insect-resistant genes in the control of this group of pests .

其中一些基因已被成功地转入植物体内,并且获得的转基因抗虫植物对同翅目害虫的生长,发育,繁殖能力等方面都具有一定的抑制作用,表现出这些抗虫基因在防治这类害虫中的应用潜力。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物,经过扩增前导序列10~34aa基因序列,并重新克隆入质粒pRSET-A构建串联二聚体后,再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点,经一系列简单的酶切和连接,快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因,并成功表达其六聚体重组蛋白。

Aphids belong to Diptera insect pests; they damage plants directly by sucking juice of phloem, and indirectly by spreading plant disease. The plant lectins are proteins that bind reviviscence to specific mono- or oligosaccharides; therefore they have potential in plant genetic engineering.

本研究还克隆了植物凝集素基因,以解决棉花蚜虫的危害问题,蚜虫属同翅目昆虫,通过吸取植物韧皮部的汁液造成直接危害,又通过传播植物病害对植物造成间接危害,植物凝集素是一类具有特异糖结合活性的蛋白,在植物基因工程中具有较大的应用潜力。

Through determining leaves of Bt transgenic tobacco and leaves, flowers, bolls and bracteal leaves of Bt transgenic cotton, it is found that ELISA method is relevant to bioassay.

用建立的ELISA方法检测了转Bt杀虫蛋白基因烟草的叶片和转Bt杀虫蛋白基因棉花的叶片、花器、铃和蕾等部位,并同生物测试法相比较,发现两种方法有很好的相关性。

According to the phylogenetic analyses, the homologous genes of Buxus belong to their expected clades, showing a position near the base of core eudicots.

另外,根据谱系分析的结果,鉴定出的黄杨花部基因分别属於ABCE 同的基因系群内,位於真双子植物之中。

Introducing into to wheat the gna gene that encodingsnowdrop lectin(Galanthus nivalis agglutinin;GNA),a toxic protein to Homoptera,or cryIA gene that encoding Bacillus thuringiensis insecticidal protein forLepidopteraby genetic transformation will produce new germplasm that maybe produce resistant to somekinds of pests.

利用转基因技术,将对同翅目昆虫具有抗性的雪花莲凝集素基因gna和对鳞翅目昆虫具有抗性的苏云金芽孢杆菌杀虫蛋白基因cryIA导入小麦,极有可能获得对某些害虫具有一定抗性的小麦新种质。

The colony morphology, growth rate and homothallic characters were all steadily inherited in both single-zoospore and single-oospore progenies,which indicated that the genetic factors of controlling the three characters were homozygous.

结果表明,菌落形态、生长速率和同宗配合性状在单孢后代可稳定遗传,控制上述性状的遗传因子是纯合的;大豆疫霉菌的游动孢子产生能力在单孢后代发生连续性变异,表明这种性状可能是数量遗传性状,也可能控制这种性状的基因为杂合基因或细胞质遗传因子。

The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物,经过扩增前导序列10~34aa基因序列,并重新克隆入质粒pRSET-A构建串联二聚体后,再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点,经一系列简单的酶切和连接,快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因,并成功表达其六聚体重组蛋白。

The results showed that among Asia countries: Indian, burma, China, Pakistan and Kirghiz Republic,Hepatitis E virus genome shifted within the range of 5% and amino acid residues shifted within 1% compared with Mexico isolate.

氨基酸在测定的区域内未发现变异,与美洲发现的墨西哥株相比,亚洲株拟来自同祖先,基因和氨基酸水平的变化仅属基因漂移的范围。

Results were shown as followings:(1) Sodium selenite at 0~2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5~2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下:(1)0~2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力;而对SMMC-7721细胞的作用不显著;(2)该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度;而对SMMC-7721细胞的作用均不显著;(3)高浓度硒(5μmol/L以上)显著性抑制L-02细胞生长、致细胞过氧化,灯盏花素能拮抗硒所致过氧化、降低硒毒性;(4)0.5~2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧,同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著;(5)富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白,其结构和功能有待研究;(6)通过已有的含hTERT基因质粒,成功构建hTERT反义RNA表达质粒,转染SMMC-7721细胞后对其生长具有抑制作用。

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In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国,慢性酒精中毒,肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题,你可以随时联系我。

Very pretty, but the airport looks more fascinating The other party wisecracked.

很漂亮,不过停机坪更迷人。那人俏皮地答道。