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Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础,构建了原核表达载体PQE30a/FeSOD,经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后,再转化入大肠杆菌M15中,通过IPTG诱导,得到高效体外表达,经SDS-聚丙烯酰胺凝胶电泳检测,表达的目的蛋白占总菌体蛋白的37%,可溶性和包涵体两种形式均有存在,上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析,表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

Containing or treated with carbolic acid .

一种必需氨基酸,为亮氨酸的同分异构体。

A structural isomer of citral that is obtained from the oxidation of nerol and is used to make perfumes and flavorings.

橙花醛柠檬醛的同分异构体,橙花醇氧化可得,用于制造香水和香料

Methods 12 guinea pigs of 20 months with 3 cm×3 cm hair sheared from their backs were divided into control group and experimental group. Rhodosin and deer serum preparation was besmeared on the skin of guinea pigs in experimental group, and cosmetic matrix was besmeared on the skin of animals in control group for 60 d. The distribution of type Ⅲ collagen was observed with Sirius red staining and HE staining. The fission index of the cells of stratum basale, the thickness proportion of stratum corneum to epidermis, the volume density of the collagen fibers and small blood vessel in dermis, the number of fibroblasts in aging skin were meassured with technique of sterology.

背部被剪去3 cm×3 cm毛的12只20月龄豚鼠被随机均分为给药组和对照组,给药组涂抹红鹿制剂,对照组涂抹化妆品基质,连续涂抹60 d;应用天狼猩红、HE染色和体视学技术分别观察和测定老化皮肤中Ⅲ型胶原的分布、表皮基底层细胞的分裂指数、角质层同表皮厚度的比例、真皮胶原纤维和微血管的体密度和成纤维细胞的数目。

In this thesis, a series of complexes based on aromatic multicarboxylic acids have been successfully synthesized in solutions or under hydrothermal conditions. Their structure and properties are investigated.(1) Eight complex compounds have been synthesized and characterized by X-ray single crystal diffractive technology: The eight complexes are listed as following: [Cu242] complex 1 [Cd22(H2O)4]·4H2O complex 2 [Co(H2btc)(H2O)3] complex 3 [Co2(H2O)2]·H2O complex 4 [Ni22(H2O)4] complex 5 [Cu22(H2O)4] complex 6 [Co(H2biim)2(H2O)2](H2btc) complex 7 [Zn(H2biim)2(H2O)2](H2btc) complex 8 The structure of complex 1 is dinuclear complex resulted from weak interactions(0-D chain); complex 2 is 1-D chain stucture result from interactions of water molecules; complex 3、4、5、6 are coordination polymers using hydrothermal synthses, where the first kind ligand is H4btc, the second kind ligand is phen and Co2+、Ni2+、Cu2+ as center ions, respectively. While the coordination enviroment of Co2+ is the same in complex 3, the coordination geometries around the Co atoms in complex 4 are obviously different because of the different reaction conditions. In complex 4, the 1-D chains are connected into 2-D layer through carboxy groups of ligand H4btc. The structures of complex 5、6 are 1-D chain stucture result from interactions of carboxy groups in ligand H4btc. Complex 7、8 are homeomorphy compounds. Either of them are linked to the 3-D chains through intermolecular hydrogen bonds. Each H4btc lose two protons and H2btc2- acts as negative electron balance.

合成了8个结构新颖的配合物,并用X-射线单晶结构分析方法确定了晶体结构,分别为: [Cu242] 配合物1 [Cd22(H2O)4]·4H2O 配合物2 [Co(H2btc)(H2O)3] 配合物3 [Co2(H2O)2]·H2O 配合物4 [Ni22(H2O)4] 配合物5 [Cu22(H2O)4] 配合物6 [Co(H2biim)2(H2O)2](H2btc)配合物7 [Zn(H2biim)2(H2O)2](H2btc)配合物8 配合物1是一个依靠弱作用连接的双核铜结构;配合物2借助水分子形成一维链状结构;配合物3、4、5、6是以H4btc为第一配体、phen为第二配体,通过水热法合成的配合物,其中,Co2+、Ni2+、Cu2+为中心离子;配合物3中的二价钴离子具有相同的配位环境,不同反应条件下得到的配合物4中的二价钴离子存在不同的配位环境,在配合物4中,一维链通过H4btc上的羧基形成一个二维层结构;配合物5、6是借助H4btc上的羧基形成的一维链状结构;配合物7、8属于异质同晶结构,它们的分子通过分子间氢键形成三维网状结构,H4btc上的羧基失去2个质子,作为一个二价负离子起到电荷平衡作用。

SiC polytypes of 6H and 15R and a transition zone between the two were observed under HREM and TEM and discussed from the point view of the crystal structures; the clear evidence of Al〓C〓 nucleated in the SiC particle was provided, indicating the aluminum penetrating the SiC grains is already carbon-saturated and, consequently, Al〓C〓 crystals grow in the particles wherever a temporary local supersaturating is produced; The experimental observation indicated that the types of the interface between SiC and Al are variable and the distribution feature of reaction product, Al〓C〓, were also given in the present work: to be nucleated on SiC, to be aggregated at SiC/Al interface zone or to be aggregated at the crystal boundaries.

通过高分辨透射电镜,本文观察到SiC增强体中6H和15R同质多晶现象及6H-15R SiC转变区,以及另外一种无序的SiC同质多晶,并从其晶体结构的角度解释了6H和15R在晶体内共存的现象。通过高分辨透射电镜,本文给出了Al〓C〓在SiC颗粒内部形核的明证,表明Al〓C〓形核是Al渗透到SiC颗粒内部在C过饱和处在SiC的(0006)面上而形核,而且一种可能的位相关系为A1〓C〓[11〓0]∥SiC[11〓0]。研究结果同时给出了SiC/Al复合材料的界面反应产物A1〓C〓的分布特征:在SiC上形核并生长,聚集于SiC/Al界面去附近,或者聚集于晶界上。

For reconstruction from contours, this dissertation uses Active Contour Model to check the boundary contours. The algorithm can generate single, continuous and close contours quickly. For reconstruction from voxel, this dissertation presents an algorithm to trace the boundary contours from segmented regions based on Active Contour Model. The algorithm can trace the contours of inter and outer connected multi-regions correctly and accurately, and can prevent tracing from jumping out to the neighbor region, also can define the contour precision and contour key points actively as snake is growing up.

对于基于轮廓线的表面重建,利用主动轮廓模型(Active Model,又称Snake Model)来完成断层图象上目标轮廓的提取,可以获得单象素连通的封闭的目标边界轮廓;针对基于体素的表面重建中实施了分割后的体数据,提出了一种基于主动轮廓模型完成二值图象中轮廓跟踪记录的方法,可以对同属于重建目标的内外邻接多区域进行正确和准确的边界轮廓跟踪,使得在跟踪当前轮廓时不会跨越到相邻轮廓,且主动轮廓的精度可以根据参数交互决定,尤其是能够在主动轮廓成长的同时主动定义轮廓关键点。

X+_ Following host injury, the balance between the epimers and isomers is a determining factor in the overall regulation of hematopoiesis, TH/TH(2) balance, and host resistance to infections and tumor growth.

在宿主损伤以后,雄甾烯的异构体和同分异构体之间的平衡是全部生血作用的控制、平衡细胞/体液免疫、宿主抗感染和抗肿瘤增殖的决定因素。8p*f:kz\-JT6Z,l

Please not that a 250 mm wide die opening will produce a film or sheet with less width since you have a "neck in" effect when you pull out the melted resin from the die before it reaches the surface of the chill roll. the width you can achieve depends on the melt strength of the resin as well as the distance between the die lip opening and the chill roll. but in general, about 80% of the die opening width can be achieved.

注意:250毫米宽开口的模头只能获得小于此宽度的薄膜或片材,因为当熔体树脂被从模头拉至冷辊上时会产生&颈缩&效应,最终获得的实际宽度依赖于树脂的熔体强度以及模唇开口同冷辊之间的距离,但总而言之,可获得模头开口宽度80%的产品宽度。

In the study of isospin-dependent nucleon-nucleon elastic crosssections in nuclear medium we find the medium correction of nucleon-nucleon scatteringcross sections is also isospin dependent,〓 depends on the baryon density weakly and 〓 depends on the baryon density significantly.

用QHD-Ⅱ模型研究了介质两体截面的同位旋相关性,研究结果表明截面的介质修正也是同位旋依赖的:中子-质子介质截面对核密度的依赖很弱,而中子-中子的介质截面对核密度的依赖很强。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。