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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在桑葚胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

According to the relationship of Yanshan epoch granites and NE ductile shear zones of main structure of this region, and regional isotope chronology data, the granitoids of this region are divided:(1) pretectonic, qnartze monzonite -granites assemblage, representative plutons: Shigujian, Erwan, Egongbao, and Yunfengding;(2) syntectonic granite assemblage, representative plutons: Tiantangzhai, and Jiuzihe;(3) posttectonic granite assemblage, representative plutons: Daguping and Bijiashan. These assemblages differ from precollision , syncollision and postcollision intrusive assemblages.

根据燕山期花岗岩与区内主体构造NE向韧性剪切带的关系,并结合区域同位素年代学资料,将区内花岗岩分为:(1)构造前石英二长岩—花岗岩组合,代表性岩体有石鼓尖、二湾、鹅公包、云峰顶;(2)同构造花岗岩组合,代表性岩体有天堂寨、九资河;(3)构造后花岗岩组合,代表性岩体有大孤坪、笔架山,这里的构造前、同构造和构造后侵入岩组合不同于碰撞前、同碰撞和碰撞后侵入岩组合。

The results showed that POD activity increased at first and then decreased in the skin and flesh of root. The peak occurred at 40 d and 60 d in the skin and flesh, respectively. POD activity in the skin was higher than that in the flesh at 30 d and then contrary results were observed. The higher POD activity was, the more POD isoenzyme was. The pattern of POD isoenzymes was different between skin and flesh at different vegetative growth stages. POD isoenzymes in the skin were composed of five monomers and four dimers while five dimers or two monomers, two dimers and one tetramer in the flesh. POD isoenzymes in the root were mainly acidic. POD localized mainly in the cell walls of periderm, cambium, xylem ray, xylem vessels and primary xylem.

结果显示,心里美萝卜营养生长期肉质根的皮和肉中POD活性均先升高后降低,分别在播种后第40 d和60 d出现峰值,30 d时皮中POD活性高于肉,之后则相反,酶活性最高时同工酶数目也最多,不同时期,皮和肉中同工酶种类不同;皮中POD同工酶由5个单体和4个二聚体组成,而肉中由5个二聚体或2个单体、2个二聚体和1个四聚体组成,均以酸性同工酶为主;组化定位显示POD主要分布在肉质根的周皮、形成层、木射线、木质部导管和肉的初生木质部等的细胞壁附近。

The delay time of vertical coseismic deformation have positive linear relationship with ep...

断层体垂直向同震形变延迟时间与震中距线性正相关,发生地震同震形变的响应速度约为5.5km/s,反映了在上层花岗岩地层中面波的传播速度;同震位移最大振幅和震中距的对数、震级之间具有明显线性关系,震级越大,同震位移的最大振幅越大;地震震级是断层体垂直同震形变振动持续时间的主要影响因素,震级越大,同震形变振动持续时间越长。

And the concept of androgyny/bisexuality gradually stands fora perfect personality which deconcentrates sex-typing and sparkplugsdiverse individuals.

此双性同体不同于古老的男女双性结合,在词语构成上即表现出社会性别特征的多样性。

Methods Porous heterogenous deproteinication boneof pig obtained by physiochemical process and osteoblast acquired from the cranical bone of fetal rabbits were cultured 10 days in vitro and explanted into the left side of dorsa muscle of 30 adult rabbits.

将经过物理化学方法处理制得的冻干猪脱蛋白型松质骨与胎兔颅骨来源的成骨细胞体外培养 10d后,在无菌条件下植入 3 0只成年新西兰兔背部肌肉内,对照组为同体单纯植入猪脱蛋白型松质骨。

With a method similar to chandlers, artificial red and white thrombi of rabbit in vitro were prepared, and then separately infused into jugular vein of the same rabbit to form red and white thrombi in its pulmonary circulation.

采用与chandler氏相似的方法,制取家兔体外人工红、白血栓,然后分别注入同体家兔颈静脉,使之形成肺循环红、白血栓。在48小时内,给予实验动物某种活血化瘀中药或对照生理盐水。

Thirty-six rats ,weighing(250±10)g, were randomly divided into normal group, experimental group and control group.Orthodontical devices were put between the upper incisor teeth and dens molaris in both experimental group and control group.The corrective force was adjusted to 60g.Salvia miltiorrhiza combination was implanted to each rat in the experimental group every day.The animals of both experimental group and control group were killed at 7,14 and 21 days.The upper dens malarias and periodontal tissue slices were observed under the light microscope and transmission electro microscope,and the X-ray dental films were taken and measured with digitization dental scanning apparatus and its software.

以36只体重(250±10)g的雄性大鼠为样本,随机分为正常组、实验组和对照组,设定不同浓度丹参复合膜,以同体对照方式在正常组进行体内植入实验,在2周内观察不同浓度丹参的牙周组织诱导作用,寻找最佳应用浓度;在实验组和对照组的上切牙与磨牙之间隙安装正畸装置,建立大鼠磨牙移动实验模型,矫治力的大小为60g;实验组每日每只体内植入丹参复合生物膜,两组动物于正畸加力7、14、21d,分三批处死;处死后立即切取双侧上颌磨牙及牙周组织制取标本,制片透射电镜和光镜观察;拍摄X线牙片,用数字化牙科扫描仪及软件对其进行测量分析。

The corrective force was adjusted to 60g.Salvia miltiorrhiza combination was implanted to each rat in the experimental group every day.The animals of both experimental group and control group were killed at 7,14 and 21 days.The upper dens malarias and periodontal tissue slices were observed under the light microscope and transmission electro microscope,and the X-ray dental films were taken and measured with digitization dental scanning apparatus and its software.Result:The most fitting concentration of combined biological membrane was 0.80g;the speed of the remodeling of the periodontal tissue at the compression side in the experimental group was faster than that of the control group.

以36只体重(250±10)g的雄性大鼠为样本,随机分为正常组、实验组和对照组,设定不同浓度丹参复合膜,以同体对照方式在正常组进行体内植入实验,在2周内观察不同浓度丹参的牙周组织诱导作用,寻找最佳应用浓度;在实验组和对照组的上切牙与磨牙之间隙安装正畸装置,建立大鼠磨牙移动实验模型,矫治力的大小为60g;实验组每日每只体内植入丹参复合生物膜,两组动物于正畸加力7、14、21d,分三批处死;处死后立即切取双侧上颌磨牙及牙周组织制取标本,制片透射电镜和光镜观察;拍摄X线牙片,用数字化牙科扫描仪及软件对其进行测量分析。

When aborted rats in group E were treated with the dose of 50 IU/g IFN-γ, expression of TNF-αin this group was compared with the other four groups, the results were as follows: Compared with group A, the nucleus pre-opticus medialis, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami and pars intermedia had a highly expression of TNF-α, in the other nuclei of hypothalamus, neurohypophsis and adenohypophysis, the change was not significantly, the expression of TNF-αin follicle and corpus luteum were decreased distinctly, the variation in uterus was slight and no difference was exsited; Compared with group B, the expression of TNF-αin nucleus nucleus lateralis hypothalami, nucleus ventromedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and uterus presented a remarkable enhancement, conversely, the TNF-αexpression in the nucleus pre-opticus medialis, nucleus pre-opticus suprachiasmaticus, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, neurohypophsis, follicle and corpus luteum had been significantly decreased; Compared with group C, the nucleus pre-opticus medialis, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis, pars intermedia and uterus had upregulated the expression of TNF-α, however, it was decreased in the nucleus periventricularis hypothalami and corpus luteum; When compared with group D, the expression of TNF-αin nucleus lateralis hypothalami, nucleus dorsomedialis hypothalami, nucleus ventromedialis hypothalami, pituitary, follicle and ovary stroma were obviously enhanced, while in the nucleus supra-opticus and nucleus arcuatus hypothalami, the situation was definitely opposite, in the other parts, the change was not significant.

外源腹腔注射50 IU/g IFN-γ后,同A组相比:E组TNF-α免疫阳性产物在下丘脑视前内侧核、视前大细胞核、室周核、腹内侧核、背内侧核、弓状核、垂体中间部显著增高,在下丘脑其它核团、神经垂体、垂体前叶较A组差异不显著,在卵泡、黄体中阳性产物表达均较A组显著降低,在子宫中表达较A组变化不明显;同B组相比:E组TNF-α免疫阳性产物在下丘脑外侧核、腹内侧核、弓状核、垂体前叶、子宫均显著增高,在视前内侧核、视交叉上核、视上核、视前大细胞核、室周核、室旁核、神经垂体、卵泡、黄体中阳性物质表达均显著降低;同C组相比:E组下丘脑视前内侧核、腹内侧核、背内侧核、弓状核、垂体前叶与中间部、子宫均显著增高,在室周核、黄体中均显著降低;同D组相比:E组下丘脑外侧核、背内侧核、腹内侧核、垂体各部、卵泡、卵巢基质均显著增高,在视上核、弓状核显著降低,在其它部位表达变化差异不显著。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。