同体

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

The biological analysis results showed: Population Doubling Time of cells was approximately 24 h;Diploid account for 76%～88% in cell's chromosome;By Isozyme analysis of Lactic Dehydrogenase,Malic Dehydrogenase,cross contamination had not taken place;The test results of ...

结果表明：细胞群体倍增时间约为24 h；细胞染色体中二倍体(2n=60)占主体，镜检细胞的百分比为98.2%；乳酸脱氢酶、苹果酸脱氢酶同工酶分析，没有其他物种污染；细菌、真菌、病毒、支原体检测阴性。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

Methods: Both 58 simple obese male adolescents and 46 normal-weight controls were subjected to exam the natural lengths of penis, mean volume of testes, the first time of spermatorrhea, pubic hair stage. The levels of luteinizint hormone, follicle-stimulating hormone, total testosterone, estradiol (E 2), PRL and fasting insulin concentrations in serum were detected by RIA.

测量5 8例单纯肥胖青少年和4 6例同年龄、同身高体重正常者的体重指数、臀围比值、阴毛发育程度、首次遗精发生率、阴茎大小、睾丸体积，放免法测定血清黄体生成素、卵泡刺激素、总睾酮、雌二醇(E2 )、催乳素和空腹胰岛素水平。

Data were analyzed from photographs from optical microscopy and Computer Image Analysis.Results:Gene expression level of bcl-2mRNA (numeric density and concentration of bcl-2 immunopositive neurons) in layer Ⅲ of the ipsilateral visual cortex area 17 and the laminas receiving ipsilateral geniculate input from the strabismic and deprived eyes are significantly lower than the input from the untreated eyes.

MS1和MD1组幼猫实验眼同侧视皮质17区Ⅳ层及同侧外侧膝状体Al层神经元在视觉发育敏感期内，由于长期的斜视及形觉剥夺效应以致功能低下，对Bcl-2调节的敏感性降低，以及对NTs的营养保护及上调Bcl-2 mRNA表达水平应答能力降低，在双眼竞争的神经元发育及突触连接中处于劣势，使对侧眼形成优势循环。

The results showed that the electrophoretogram patterns of isozymes, serum and muscle protein of Bumper crucian carp were the same as those of its female parent and significantly different from its male parent.

结果发现：异源子代的血清、肌肉蛋白和同工酶的电泳图谱与母本彭泽鲫相同而与父本海鲤显著不同，推测异源精子的作用可能是在DNA水平上对后代产生影响，这种影响没有表现在成体同工酶的表达上。

SiC polytypes of 6H and 15R and a transition zone between the two were observed under HREM and TEM and discussed from the point view of the crystal structures; the clear evidence of Al〓C〓 nucleated in the SiC particle was provided, indicating the aluminum penetrating the SiC grains is already carbon-saturated and, consequently, Al〓C〓 crystals grow in the particles wherever a temporary local supersaturating is produced; The experimental observation indicated that the types of the interface between SiC and Al are variable and the distribution feature of reaction product, Al〓C〓, were also given in the present work: to be nucleated on SiC, to be aggregated at SiC/Al interface zone or to be aggregated at the crystal boundaries.

通过高分辨透射电镜，本文观察到SiC增强体中6H和15R同质多晶现象及6H-15R SiC转变区，以及另外一种无序的SiC同质多晶，并从其晶体结构的角度解释了6H和15R在晶体内共存的现象。通过高分辨透射电镜，本文给出了Al〓C〓在SiC颗粒内部形核的明证，表明Al〓C〓形核是Al渗透到SiC颗粒内部在C过饱和处在SiC的（0006）面上而形核，而且一种可能的位相关系为A1〓C〓[11〓0]∥SiC[11〓0]。研究结果同时给出了SiC/Al复合材料的界面反应产物A1〓C〓的分布特征：在SiC上形核并生长，聚集于SiC/Al界面去附近，或者聚集于晶界上。

As to the old-new effects in the episodic retrieval,there are similar pattern on the old-new effects of the picture-word and word-word identical matches,but the old-new effect of picture-picture identical matches shows different characteristics,especially after 600ms.

3在情景提取的旧新效应上，图-词跨形式匹配和词词同形式匹配表现出一致性，而图图同形式匹配加工的旧新效应表现出不同的特点，主要体现在600ms之后。

Business Scope: 1, ONLY licensing carbide circular saw blades, the products manufactured using imported materials, as compared with the same price products to extend the service life of more than 10%; applicable to all kinds of wood (including solid wood, particleboard, MDF and various MDF), aluminum alloy, rigid plastic, acrylic and other materials cutting; 2, the Company may in accordance with customers cutting materials and cutting of different specifications to choose custom-made special carbide circular saw blades; 3, In order to fully utilize resources, reduce costs, the company offers carbide tooth circular saw blades for the overall processing services; 4, the company's carbide circular saw blades used to provide full-service customers, in view of: a, blade matrix used for a period of time will produce deformation; b, saw in the use of the course will be part of tooth loss; c, saw the use of a period of time would not sharp blade; above phenomenon will not only greatly reduce blade life, at the same time will affect the use of effect.

ONLY牌硬质合金圆锯片，该产品全部采用进口材料制造，与同价格产品相比使用寿命延长10%以上；适用于各种木材（包括实木、刨花板、中纤板及各种密度板）、铝合金、硬质塑料、压克力等材料的切割；2、本公司可根据客户切割材料和切削方式的不同选择订做特殊规格硬质合金圆锯片；3、为充分利用资源、降低成本，本公司提供硬质合金圆锯片整体换齿加工服务；4、本公司对硬质合金圆锯片使用客户提供全方位服务，针对：a、锯片基体使用一段时间后会产生变形；b、锯片在使用过程中会出现部分齿脱落；c、锯片使用一段时间后刀刃会不锋利；以上现象出现，不仅会大大降低锯片使用寿命，同时会影响使用效果。

In the study of isospin-dependent nucleon-nucleon elastic crosssections in nuclear medium we find the medium correction of nucleon-nucleon scatteringcross sections is also isospin dependent,〓 depends on the baryon density weakly and 〓 depends on the baryon density significantly.

用QHD-Ⅱ模型研究了介质两体截面的同位旋相关性，研究结果表明截面的介质修正也是同位旋依赖的：中子-质子介质截面对核密度的依赖很弱，而中子-中子的介质截面对核密度的依赖很强。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。