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Coal, oil, wood,natural gas and fresh water1. Canada is the second largest country in the world.

形容词的最高级一般是表示"居第一位的",但也可以同序数词second ,third 连用,表示"居第二,三位的"。

The result shown is a contributing cause to the accurate operation relevant to HVA performed by surgeons. And the possibilities of successful operation in HVA have been enhanced to some extent. The method-weight-bearing AP projection of the foot can administer to choice applicable diorthosis by surgeons and believed productively to reveal the patients' real conditions.

在此研究中我们共收集40位同程的大脚趾外翻的病患,经统计后发现此40位病患足部负重摄影的HVA都大於未负重摄影,除可提供外科医生选择合适的矫正术式外,可提供医生在手术中精确的调整其HVA,术后经床追踪确实大幅提高手术的成功。

The occurrence of the granites in the Foping and vicinity correlates closely with those ductile-brittle shearing zones. The granite bodies are elongate in shape and distribute parellel to the shearing zones with high dip angles, and are plane in shape and emplace along the shearing zones with low dip angles, displaying syntectonic feature.

佛坪及其邻近地区发育的花岗岩体与这些韧性--脆韧性剪切带有密切的空间关系,即在近直立剪切带附近花岗岩体多呈现与剪切带大体平行的长条状,而在缓倾斜的低角度剪切带发育地带,花岗岩体多呈面状,且呈顺层侵位特征,显示同构造侵位特征。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank,选择包含人主要T细胞抗原表位序列的人PSCA基因片段,应用异种化抗原设计技术,保留人T细胞抗原表位,设计异种化PSCA融合抗原片段;(2)根据核酸序列按中心模板法设计引物,应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因,以PCR、限制性酶切和DNA序列测定法进行鉴定:(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点,采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4),并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中,转染293T细胞,借助免疫荧光+流式细胞术考察插入片段表达效率,最终选定PSCA_3片段进行下一步研究;(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下,插入pVAX1-neo-IRES—GM/B7载体中,构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB,并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况;(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞,制备小鼠前列腺癌模型,并采用股四头肌肌肉注射+电脉冲法(Electroporation,EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB,同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照,通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率,来评价该DNA疫苗在小鼠体内的抑瘤效果。

In this thesis, a series of complexes based on aromatic multicarboxylic acids have been successfully synthesized in solutions or under hydrothermal conditions. Their structure and properties are investigated.(1) Eight complex compounds have been synthesized and characterized by X-ray single crystal diffractive technology: The eight complexes are listed as following: [Cu242] complex 1 [Cd22(H2O)4]·4H2O complex 2 [Co(H2btc)(H2O)3] complex 3 [Co2(H2O)2]·H2O complex 4 [Ni22(H2O)4] complex 5 [Cu22(H2O)4] complex 6 [Co(H2biim)2(H2O)2](H2btc) complex 7 [Zn(H2biim)2(H2O)2](H2btc) complex 8 The structure of complex 1 is dinuclear complex resulted from weak interactions(0-D chain); complex 2 is 1-D chain stucture result from interactions of water molecules; complex 3、4、5、6 are coordination polymers using hydrothermal synthses, where the first kind ligand is H4btc, the second kind ligand is phen and Co2+、Ni2+、Cu2+ as center ions, respectively. While the coordination enviroment of Co2+ is the same in complex 3, the coordination geometries around the Co atoms in complex 4 are obviously different because of the different reaction conditions. In complex 4, the 1-D chains are connected into 2-D layer through carboxy groups of ligand H4btc. The structures of complex 5、6 are 1-D chain stucture result from interactions of carboxy groups in ligand H4btc. Complex 7、8 are homeomorphy compounds. Either of them are linked to the 3-D chains through intermolecular hydrogen bonds. Each H4btc lose two protons and H2btc2- acts as negative electron balance.

合成了8个结构新颖的配合物,并用X-射线单晶结构分析方法确定了晶体结构,分别为: [Cu242] 配合物1 [Cd22(H2O)4]·4H2O 配合物2 [Co(H2btc)(H2O)3] 配合物3 [Co2(H2O)2]·H2O 配合物4 [Ni22(H2O)4] 配合物5 [Cu22(H2O)4] 配合物6 [Co(H2biim)2(H2O)2](H2btc)配合物7 [Zn(H2biim)2(H2O)2](H2btc)配合物8 配合物1是一个依靠弱作用连接的双核铜结构;配合物2借助水分子形成一维链状结构;配合物3、4、5、6是以H4btc为第一配体、phen为第二配体,通过水热法合成的配合物,其中,Co2+、Ni2+、Cu2+为中心离子;配合物3中的二价钴离子具有相同的配位环境,不同反应条件下得到的配合物4中的二价钴离子存在不同的配位环境,在配合物4中,一维链通过H4btc上的羧基形成一个二维层结构;配合物5、6是借助H4btc上的羧基形成的一维链状结构;配合物7、8属于异质同晶结构,它们的分子通过分子间氢键形成三维网状结构,H4btc上的羧基失去2个质子,作为一个二价负离子起到电荷平衡作用。

ResultsThe glandular cell in the endometrial implant after therapy with middle or high-dose of (10,15 gkg-1d-1) XiaoChaiHu Decoction showed characteristic features of apoptosis which displayed by the cell decreased in size, karyopyknosis , cytoplasmic and nuclear chromatin condensation , density increased and a lot of apoptotic bodies among cell. Whereas some stromal cell displayed degeneration and necrosis. The protein expression of Fas and Caspase-3 in endometriotic tissue of XiaoChaiHu Decoction group was higher than that in the endometrium.

结果中、高剂量小柴胡汤(10,15 gkg-1d-1)治疗后,异位内膜有较多腺上皮细胞出现凋亡的特征,表现为细胞体积变小,核固缩,胞浆和核染色质凝集,密度增高,细胞间凋亡小体,同时间质细胞中可见一些坏死细胞;异位内膜Fas蛋白、Caspase-3蛋白的表达水平明显高于其在位内膜。

The two Ajaxes, servants of Mars, Meriones, and the son of Nestor all wanted to go, so did Menelaus son of Atreus; Ulysses also wished to go among the host of the Trojans, for he was ever full of daring, and thereon Agamemnon king of men spoke thus:"Diomed," said he, son of Tydeus, man after my own heart, choose your comrade for yourself- take the best man of those that have offered, for many would now go with you.

两位埃阿斯,阿瑞斯的伴从,愿意同行,墨里俄奈斯请愿同往,而奈斯托耳之子更是急不可待,还有阿特柔斯之子、著名的枪手墨奈劳斯。坚忍的俄底修斯亦在请缨之列,决意潜入特洛伊人的营垒,胸中总是升腾着一往无前的豪烈。其时,全军的统帅阿伽门农开口说道:图丢斯之子,你使我心里充满欢悦。你可按自己的意愿,挑选你的伙伴,择取自愿者中最好的一位,从我们济济的人选。

Monorchis belonging to Orchidaceae had a narrower one, indicating that rare and endangered Orchidaceae plants had a less distribution in Beijing mountainous region, and only distributed in several types of forest vegetation communities. Due to the lack of suitable habits, these herbage species were in dire need of prior conservation.

结果表明:野大豆的生态位宽度值较大,为0.297;大花杓兰、沼兰和角盘兰同属于兰科植物,生态位宽度值较小,说明兰科植物在北京地区分布较少,并且仅在几种森林群落类型中有分布,适宜生存的生境较少,急需优先保护。

The two target children were repeatedly measured using the "Sociometric Method" and "Behavioral Traits Assessment Questionnaire" at pretreatment posttreatment, and 4-week follow-up assessment time.

游戏团体中除两位被拒绝的目标儿童之外,亦邀请四位同侪成员加入,进行每周二次、每次四十分钟,共计十二次之游戏治疗。

For the first time I analyzed the co-existence mechanism of the two sympatric species shared the same food resource in a comparative study on the resource use and spatial niches.

首次从资源生态位和空间生态位等方面对同域分布、食性相似的两种熊猫的共存机制进行了较为全面的探讨。

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推荐网络例句

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在,'歼灭战perfuming自己与胡努特,那麼,他来到和SAT 。

The shamrock is the symbol of Ireland and of St.

三叶草是爱尔兰和圣特里克节的标志同时它的寓意是带来幸运。3片心形叶子围绕着一根断茎,深绿色。