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Working with mathematician Roger Penrose and using Einstein's relativity equations, he developed new techniques to prove mathematically that at the heart of black/holes were singularitiesinfinitely dense, dimensionless points with irresistible gravity.

他同数学家罗杰潘罗斯合作,运用爱因斯坦的相对论等式找到了新的计算方法来证明:在黑洞的中心是一堆密密麻麻的、无边无际的、具有巨大引力的奇异小点。

Working with mathematician roger penrose and using einstein's relativity equations, he developed new techniques to prove mathematically that at the heart of black/holes were singularities—infinitely dense, dimensionless points with irresistible gravity.

他同数学家罗杰·潘罗斯合作,运用爱因斯坦的相对论等式找到了新的计算方法来证明:在黑洞的中心是一堆密密麻麻的、无边无际的、具有巨大引力的奇异小点。

Work ing with mathematician Roger Penrose and us ing Einstein's relativity equations, he developed new techniques to prove mathematically that at the heart of black/holes were singularities-infinitely dense, dimensionless points with irresistible gravity.

他同数学家罗杰。潘罗斯合作,运用爱因斯坦的相对论等式找到了新的计算方法来证明:在黑洞的中心是一堆密密麻麻的、无边无际的、具有巨大引力的奇异小点。

Working with mathematician Roger Penrose and using Einsteins relativity equations, he developed new techniques to prove mathematically that at the heart of black/holes were singularities—infinitely dense, dimensionless points with irresistible gravity.

他同数学家罗杰·潘罗斯合作,运用爱因斯坦的相对论等式找到了新的计算方法来证明:在黑洞的中心是一堆密密麻麻的、无边无际的、具有巨大引力的奇异小点。

Working with mathematician Roger Penrose using Einstein's relativity equations, he developed new techniques to prove mathematically that at the heart of black/holes were singularities—infinitely dense, dimensionless points with irresistible gravity.

他同数学家罗杰·潘罗斯合作,运用爱因斯坦的相对 www.8t t t8.com 论等式找到了新的计算方法来证明:在黑洞的中心是一堆密密麻麻的、无边无际的、具有巨大引力的奇异小点。

Working wit h mat h ematician Roger Penrose and using Einstein's relativity equations, h e developed new tec h niques to prove mat h ematically t h at at t h e h eart of black/ h oles were singularities—infinitely dense, dimensionless points wit h irresistible gravity.

他同数学家罗杰·潘罗斯合作,运用爱因斯坦的相对论等式找到了新的计算方法来证明:在黑洞的中心是一堆密密麻麻的、无边无际的、具有巨大引力的奇异小点。

STRUCTURE: Titanium and composites used extensively, with particular emphasis on corrosion resistance; fully articulated three-blade coaxial contrarotatmg rotors have all-composites blades with carbon fibre and glass fibre main spars, pockets (13 per blade) of Kevlar-type material, and filler similar to Nomex; blades have non-symmetrical aerofoil section; each has ground-adjustable tab; each lower blade carries adjustable vibration damper, comprising two dependent weights, on root section, with further vibration dampers in fuselage; tip light on each upper blade; blades fold manually outboard of all control mechanisms, to folded width within track of main landing gear; rotor hub is 50% titaniuin/50% steel; rotor brake standard; all-metal fuselage; composites tailcone; fixed incidence tailplane, elevators, fins and rudders have aluminium alloy structure, composites skins; fins toe inward approximately 25°; fixed leading-edge slat on each fin prevents airflow over fin stalling in crosswinds or at high yaw angles.

结构:钛和复合材料广泛使用,耐腐蚀性能与特别强调,充分阐明三叶片转子同轴contrarotatmg有碳纤维和玻璃纤维的主要棒材全复合材料桨叶,口袋(每刀片13)凯夫拉型材料,类似的Nomex纤维填料;叶片非对称机翼部分,每有地面可调选项卡,每降低叶片振动阻尼器进行调节,包括两部分依赖重根,在机身进一步减振装置;小费每个刀片上轻;刀片倍手动舷外所有控制机制,折叠宽度内的主起落架的轨道;转子中心为50%titaniuin/50%钢;转子制动标准;全金属机身,复合材料tailcone,固定发生机尾,电梯,蛙鞋和方向舵的铝合金结构,复合材料的皮肤;鳍鳍在每个脚趾向内约25 °;固定领先的板式翅片防止了侧风或气流停滞在高偏航角度。

The common capacitance sensor is composed of two-electrode planks which vary in shape and can be fixed in different plane of space. The driving and inductive electrode pole placed in the same plane of the uniplanar scattering-field capacitive sensor. The electric field presents scattering. As the shape of power line in the electric fields is like semicircular arc or ellipse, there is some limits in the measurement depth of uniplanar scattering-field capacitive sensor, and its penetration depth is equal to 2/3 of center distance of adjacent driving and irritable electrode pole.

电容传感器是由两极板组成,极板的形状有多种,两极板一般位于不同的空间平面;而同面散射场电容传感器的驱动电极和感应电极位于同一平面,其电场呈散射状,电场的电力线近似半圆弧形或椭圆形,因而其测量穿透深度是有一定限制的,是相邻驱动电极和感应电极中心距的2/3。

Organozinc reagents can react with a variety of α,β-acetylenic ketones and aldehydes in the absence of any catalyst. The one-pot reaction affords β-branched Baylis-Hillman type adducts, the multifunctionalized alkenes, in excellent yields with high stereoselectivity.

这种顺式亲核加成反应的发生是由于该类有机锌试剂既具有较强的亲核性,又具有Lewis酸的特性,使有机锌中R基团从同位进攻中心碳原子而形成新的碳碳键。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank,选择包含人主要T细胞抗原表位序列的人PSCA基因片段,应用异种化抗原设计技术,保留人T细胞抗原表位,设计异种化PSCA融合抗原片段;(2)根据核酸序列按中心模板法设计引物,应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因,以PCR、限制性酶切和DNA序列测定法进行鉴定:(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点,采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4),并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中,转染293T细胞,借助免疫荧光+流式细胞术考察插入片段表达效率,最终选定PSCA_3片段进行下一步研究;(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下,插入pVAX1-neo-IRES—GM/B7载体中,构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB,并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况;(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞,制备小鼠前列腺癌模型,并采用股四头肌肌肉注射+电脉冲法(Electroporation,EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB,同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照,通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率,来评价该DNA疫苗在小鼠体内的抑瘤效果。

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