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Furfur. At the same time, selected the culture supernatant. Used the whole-fat-cow"s-milk plate method, the Folin-phenol method and the QuantiCleave proteinase Assay Kit to test the proteinase activity of mycelial phase and yeast phase of M. furfur. The effect of temperature and pH on this assay was also evaluated.Result:①The whole-fat-cow"s-milk plate method: Seven days after inoculation, the proteinase activity of the mycelial phase supernatant (12.5 ± 1.2mm) is higher than that of the yeast phase (9.25±0.71mm)(p.05); With the passage of the inoculation time, the proteinase activity of the mycelial phase is rising; the temperature has no obvious effect on the proteinase

结果:①全脂牛奶平板法:培养第7天菌丝相上清液蛋白酶活性(12.5±1.2mm)高于酵母相(9.25±0.71mm)(p<0.05);菌丝相上清液蛋白酶活性随培养天数延长而增大;25℃和37℃两种温度对上清液蛋白酶活性无明显影响(P>0.05);②Folin-酚法:上清液蛋白酶活性随培养天数、菌落计数、菌丝诱导率增加而增大,培养液pH值呈下降趋势;同一天的菌丝相上清液蛋白酶活性明显高于酵母相(p<0.05);三种温度(25℃、37℃、49℃)及三种pH值(3.8、5.6、7.4)对上清液蛋白酶活性无明显影响;温度37℃、pH5.6时菌丝相上清液蛋白酶活性最高;③QuantiCleave蛋白酶试剂盒:菌丝相及酵母相上清液中均含有蛋白酶且

Mr Geldof is well-known for organizing two big pop concerts on the same day.

盖尔多夫先生因在同一天组织两场大型的流行音乐会而出名。

That same day, plaintiffs from China and Japan said in Shanghai that they will continue to collect evidences and push the Japanese government to confess to the use of germ warfare during its invasion of China.

同一天,来自中国和日本的原告在上海表示,他们将继续收集证据,迫使日本政府承认在侵华期间使用过细菌武器。

I know you don't like your boss,but as long as your work contract is not terminated,you'll have to grin and bear it.

我知道你不喜欢你的上司,可是只要你的工作合同一天有效,你就得默默地干下去。

That same day in similar weather and humidity, in Cambridge, Md., nearly 1,400 athletes raced in the Eagle Man Half Ironman — a 1.2-mile swim, a 56-mile bike ride, and a 13.1-mile run.

同一天,在马里兰的堪布雷其,相似的气候条件下,大约1400名运动员参加了鹰人比赛——包含了2.1英里的游泳,56英里的骑车和13.1英里的跑步项目。32岁的艾米也在其中。

They were registered two years ago on the same day as Harry Potter and the Half-Blood Prince.

它们是与《哈利波特与"混血王子"》在两年前的同一天被登记上的。

On the same day, we also decided to bring home to Hanoi that we meant bussiness.

同一天,我们又决定让河内知道我们是认真的。

The same day, as if to emphasise his hardline credentials, Mr Lee visited the American army base in central Seoul.

同一天,似乎是为了强调他的强硬立场,李先生访问了汉城市中部的美军基地。

Here, an Orthodox Hasidic Jew walks between two Arab men at the entrance to Hebron's Tomb of the Patriarchs, a sacred shrine that functions as both synagogue and mosque, sometimes in the course of a single day.

在这里,一个东正教哈西德派犹太人在走在两个阿拉伯人之间站在希伯伦族长墓的入口处,这座神圣的墓冢起着犹太教堂和清真寺的双重作用,有时在同一天之内。

It must be sown and reaped on the same day and this I will do, henceforth.

它必须在同一天播种和收获,因此,这也将会是我的做的事情。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。