合胞体

Results: We compared the syncytium formation activities of 10 combinations of 2 effector cell lines and 5 target cell lines, respectively, and found that mixing of CHO-WT and MT-2 cells could form syncytia obviously.

结果：本研究比较了2种效应细胞与5种靶细胞的10种组合，发现将CHO-WT和MT-2细胞混合，可形成明显的合胞体。

Methods:Using cell culture and immunohistochemical techniques,the peripheral blood specimens from 33 RA patients were designed to test the human T cell lymphotropic virus type Ⅰ related antigens,reverse transcriptase and spontaneous syncytium formation.

采用细胞培养、免疫组织化学技术等对33例RA患者的外周血样本进行人类嗜T淋巴细胞病毒Ⅰ型相关抗原、逆转录酶活性测定及观察自发性合胞体形成。

Studies on the functional role of the putative receptors indicated that the isolated binding proteins of N34 (L6) C28 successfully blocked syncytium formation induced by HIV-1 envelope glycoprotein, which provided first experimental evidence for a new model of HIV-1 entry suggested by us.

功能研究证明融合活性六螺旋的潜在受体能够抑制由HIV-1包膜蛋白介导的合胞体形成，这为我们提出的HIV-1感染新模型提供了第一个实验证据。

Methods Calcein-AM stained H9/HIV-1 ⅢB cells were treated with difierent concentrations of PF in the first,second and third trimesters respectively,which was then mixed with MT2 cells and put under fluorescent microscopy for detection of HIV-1-mediated syncytium formation.MT2 cells were treated with cell-free HIV-1 Ⅲ B,and then washed and cultured with different concentrations of PF in the first,second and third trimesters respectively.Furthcrly,the protecting effect of PFs against HIV-1-infected cells Was determined with MTT,viral replication Was evaluated by measurement of the levels of p24 antigen in culture supernatants with ELISA and the inhibition rate Was calculated.

采用荧光染料Calcien-AM标记的H9/HIV-1 ⅢB分别与早、中、晚孕期不同稀释浓度的PF作用后，与MT2细胞混合培养，荧光显微镜下观察合胞体的形成；用游离的HIV-1 ⅢB感染MT2细胞，并分别与早、中、晚孕期不同稀释浓度的PF作用后，用MTT法检测HW-1感染细胞的存活率，用HIV-1 p24抗原试剂盒检测细胞培养上清中p24抗原含量的变化。

Methods:The effect of chrysin on HIV1 infection and replication in vitro were detected by cell fusion of MT2 cells and HIV1ⅢB/H9 cells labeled calceinAM and the inhibition study of syncytia formation and p24 production monitored in culture supernatant of MT2 cells infected by HIV1ⅢB, respectively. Flurescence conjugated monoclonal antibodies and flow cytometry were used to detect the expression of CD69 by activated CD4+T in response to PHA.

以CR对CalceinAM标记的慢性感染HIV1ⅢB的H9细胞（H9/HIV1ⅢB）和MT2细胞的融合、对HIV1诱导的MT2细胞形成合胞体及对感染HIV1的MT2细胞产生p24抗原的影响，探讨其抗HIV1活性；以PHA刺激人外周血单个核细胞，利用流式细胞术，检测CD4+T细胞CD69表达的百分率，分析CR对免疫活化的影响。

The results from flow cytometry assay showed that the low concentrations of LIF had the ability to shift cytotrophoblast differentiation towards a syncytial pathway, while the high concentrations of LIF towards a invasive pathway.

细胞流式仪结果表明低浓度的LIF可促进绒毛合胞体滋养层的分化，高浓度的LIF可促进绒毛外侵入性滋养层细胞的分化。

The syncytia was founded in the root of the resistant cultivar.

并且观察到抗病品种根内产生的合胞体。

The cell adaptability tests showed that the CPE caused by wild CCV strains were different from reference strains syncytia CPE.

细胞适应性试验表明，分离到的野毒在A72细胞上导致的CPE不同于参考毒株的合胞体病变，NJ2、KM株主要导致细胞圆缩、脱落，NJ2株的6-15代在A72细胞上有CPE，但是15代后不再出现CPE。

The EC_(50) of QKL against the formation of syncytia of MT-2 induced by HIV-1_ was 1/198.02 with a SI of 5.36. By detecting the survival rate of co-culturing H9/HIV-1_ cells and MT-2 cells or MT-2 cell direct infected by HIV-1_ QKL was found to be protective to cells. The EC_(50) was 1/166.67 and 1/144.93 with SI of 4.51 and 3.92 respectively. The EC_(50) of QKL against P24 antigen production was 1/175.44 with SI of 4.75. The drug serum of QKL was also found to be effective to inhibit the cell fusion and protect cells infected by HIV-1_.

结果：QKL对H9／HIV-1_细胞和MT-2细胞的CC_(50)分别为1／50.76和1／36.97；抑制H9／HIV-1_细胞和MT-2细胞早期融合的EC_(50)=1／235.29，SI=6.36；抑制HIV-1_细胞诱导MT-2细胞形成合胞体的EC_(50)=1／198.02，SI=5.36；H9／HIV-1_细胞和MT-2细胞混合培养和HIV-1_感染MT-2细胞时，QKL保护病毒感染细胞免于死亡的EC_(50)分别为1／166.67和1／144.93，SI分别为4.51和3.92；抑制p24抗原产生的EC_(50)=1／175.44，SI=4.75；QKL药物血清也可抑制H9／HIV-1_细胞和MT-2细胞的早期融合，保护细胞免于死亡。

Two fragments G1 and G2 of the glycoprotein G gene of bovine respiratory syncytial virus were selected for expression in Escherichia coli based on the analysis of glycoprotein G by DNA Star software.

经生物学软件DNA Star分析，将牛呼吸道合胞体病毒G基因截短成2个片段G1和G2。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。