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Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证明。

Nectar glands confluent, subtending bases of all stamens.

蜜腺合流河,全部雄蕊的对着基部。

Nectar glands confluent, subtending bases of filaments.

蜜腺合流河,花丝的对着基部。

Nectar glands confluent, subtending bases of all filaments.

蜜腺合流河,全部花丝的对着基部。

Firstly, different merging models of mixed traffic flow on conditions of different superior flow headways were advanced according to existing analyzed conclusions on traffic characteristic of on - ramp freeway junction.

利用已有的高速公路合流区外倜车道交通特征分析结论,推求在外侧车流不同车头时距分布特征下的上匝道混合车流汇合概率模型。

Different merging mode- is of mixed traffic flow on conditions of different superior flow headways were advanced according to existing analyzed conclusions concerning the on-ramp traffic characteristic.

利用已有的高速公路合流区外侧车道交通特徵分析结论,推求在外侧车流不同车头时距分布特徵下的上匝道混合车流汇合概率模型。

Because the cape stands at the meeting point of warm and cold currents, sea fog is often created around it. The entire cape has been designated as Hidaka-sanmyaku-Erimo Quasi-National Park.

这里是寒流与暖流的合流点,容易出现海雾,整个海岬被指定为日高山脉襟裳国定公园。

Using two-color immunofluorcscence flow cytometry,we analyzed expression of active antigens of normal human peripheral blood T cells.

作者利用双色荧光试剂,结合流式细胞术分析了16例正常人外周血T细胞表面活化抗原的表达。

And the meeting of Zen Buddhism and art has injected special connotation and philosophic interest into Chinese art .

而禅宗与艺术的合流为中国艺术注入了特定内蕴和哲理情趣。

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The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0,0.4,0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗,测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

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Have a good policy environment, real estate, secondary and tertiary markets can develop more rapidly and improved.

有一个良好的政策环境,房地产,二级和三级市场的发展更加迅速改善。