合核体

Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法：成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒，手术后雄鼠与超排雌鼠合笼交配，用荧光原位杂变(Fluorescence in situ hybridization，FISH)分别检测单细胞胚和二细胞胚间期核中HBV DNA的存在与复制，用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction，RT-PCR)和免疫荧光方法检测HBV基因在二细胞胚胎中的表达。

In 8 cases, both the results were consistent excepting for minor discrepancies.③In 3 cases, including 2 each with triploidy and tetraploidy respectively, and one with chimeric karyotype of normal/+22, the results from the two methods were discrepant.

CGH与常规R带的分析结果比较可分为三种：①CGH和常规核型分析结果完全一致的有3例；②CGH分析和R带分析从不同角度提供了相对一致信息的有8例；③CGH分析和R带分析结果不一致的有3例，其中2例分别为约三倍体和约四倍体，另1例为正常/＋22嵌合型。

Methods n-Cetyl-l-thio-β-D-galactoside was synthesized by a series of reaction from galactose and incorporated into Small Unilamellar Vesicles which was prepared by sonication at the ratio for L-α-PC∶Chol∶L-α-PE as 7∶2∶1, with or without 10 mol%Cetyl-gal.

半乳糖经乙酰化、溴化、缩合、亲核取代反应制备半乳糖新糖脂质并与其他脂质制备脂质体，在二氯化锡还原作用下用99mTc标记脂质体，进行动物体内靶肝实验，用γ计数器检测各脏器放射性，计算摄取率。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新