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In order to express the recombinant peptide of both gp52 and pp150 Oterminal peptides from human cytomegalovirus in the Pichia pastoris, the recombinant plasmids were transformed into Pichia pastor is by electroporation after linearised respectively by Sac I or Bg1II, the transformants were screened in MD plates without histidine.

为了在巴斯德毕赤酵母中表达人巨细胞病毒gp52C末端和pp150C末端串联片段的嵌合肽,将构建好的hCMVp-pPIC9K重组质粒分别经Sac Ⅰ和Bg1 Ⅱ酶切,电打孔法导入毕赤酵母GS115后,在缺组氨酸的MD板上筛选出转化子。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?