合成物质

In ovary, PNA receptors appeared in the oocyte cytoplasm of second phases of oogenesis; the positive granules gradually increased from third to forth phases, and they exhibit a maximum expression before vitellogennic stage in cytoplasm of oocyte; from vitellogennic stage to chorionation stage, the positive granules gradually reduced. Binding sites on follicle cells were changed with their morphological variation in every stage of oogenesis.The staged and specific expression of oncogene c-kit, the tyrosine-kinase receptor, is closely related with gametogenesis.

2在中华蚱蜢卵子发生的早期，PNA受体是由卵母细胞自身合成；在卵黄发生前的准备时期，卵母细胞质中的PNA受体一部分来源于自身合成，另一部分则来源于滤泡细胞，而糖复合物的大量出现可能直接与卵黄发生有关；卵黄发生期PNA受体的减少可能是在卵黄物质形成中部分发生了修饰；卵黄和卵壳的相继形成过程中，阳性反应的出现说明PNA受体参与了卵黄膜及卵壳的形成，而这些受体物质是由滤泡细胞分泌的。

In addition, the preparation and package and store for standard reference materials of antioxidant BHT are studied.

本论文主要叙述了对工业品防老剂BHT的重结晶提纯和区域熔融提纯(自行设计和制造了区域熔融仪，申请了发明专利)来制定防老剂BHT标准物质的研究：通过实验确定了乙醇重结晶提纯工艺；利用自行设计区域熔融装置，拟定操作规程和技术条件；确定提纯样品的高效液相色谱法和差示扫描量热法的纯度检测条件和实验方法；用高效液相色谱检验BHT标准物质的均匀性、稳定性；分别用高效液相色谱法和差示扫描量热法测定这种标准物质的不确定度评定及两种不同原理检测的定值分析；最后确定标准物质BHT的纯度和合成标准不确定度：标准物质的制备、包装及贮存。

Furthermore,enzymatic production of cephalexin using free penicillin G acylase in aqueous system wasstudied. The parameters, including pH, temperature, concentration of 7-ADCA and molar ratiosof 7-ADCA to phenylglycine methyl ester, that affect the yield of cephalexin, wereevaluated. In section Ⅱ, the integration technique of bioseparation and biosynthesis was investigatedfirst time in the enzymatic production of cephalexin.

第Ⅰ部分主要建立了对酶法合成头孢氨苄过程行之有效的HPLC检测方法，实现了头孢氨苄合成过程中所涉及的四种物质，即头孢氨苄、苯甘氨酸甲酯、苯甘氨酸及7-ADCA快速有效的检测；同时对水相体系中头孢氨苄合成过程的影响因素进行了研究，明确了水相体系中温度、pH值，底物浓度以及底物配比对酶法合成头孢氨苄得率的影响。

Spraying with chitosan of different concentration significantly increased the content of chlorophyll ,anthoxanthin ,chlorogentic acid, polyphenal ,soluble protein and total soluble sugar of Broccoli flowers. 40 mg L-l chitosan showed the most remarkable effet,compared to control, the content of chlorophyll ,anthoxanthin ,chlorogentic acid, total soluble sugar and soluble protein of treated Broccoli flowers increased by 64.4%,282.5%,35.6%,16.2% 22.7% respectively.PAL and PPO are key enzymes in synthesize of phenolics.No relation was found between phenolics synthesize and PAL activity in Broccoli flowers,but it showed positive relation between phenolics synthesize and PPO activity.

研究表明，本研究中，不同浓度几丁聚糖喷施对青花菜花球的叶绿素、类黄酮、绿原酸、多酚总量、可溶性蛋白及可溶性总糖的含浙江大学硕卜学位论文(2004)量有显著增加，其中以浓度为40mgL一'影响最为显著，花球中的叶绿素、类黄酮、绿原酸、可溶性总糖、可溶性蛋白分别较对照提高64.4%、282.5%、35.6%、 16.2%、22.7%，苯丙氨酸解氨酶、多酚氧化酶是酚类物质合成中关键酶，花球内酚类物质合成与苯丙氨酸解氨酶的活性没有一定的相关性，而与多酚氧化酶的活性呈正相关。

Electrosynthesis is a new method for preparation of αalanine. There is few reference about it, but electrosynthesis has a high selectivity and a high current efficiency. In addition, the operating condition is mild, and the reaction process is easy to be controlled.

有关电化学法合成α—丙氨酸的文献仅有两篇，虽然这方面的研究很不充分，但是电合成法避免了剧毒物质的使用，操作条件温和，通过选择电极材料、电极电位和电流密度等电化学参数，能够控制合成发应的进程，而且电合成可以具有很高的选择性和电流效率。

When used in superabrasive grits synthesizing, especially in the case of cubic boron nitride, the consumption of anvils can be decreased effectively; and the quality and yield of CBN can he improved.

在超硬材料合成中，尤其是立方氮化硼合成中，能有效降低锤耗，同时可提高立方氮化硼的合成品质及产量；超硬材料表面镀钛及钛合金后，能实现镀层和结合剂的冶金结合，工具寿命提高30%以上；钛对许多氧化物质和还原物质具有极好的耐腐蚀性，适用于人造金刚石的批量电解提纯工序。

Inthe whole process, the oocytes, nurse cell and follicle cell morphology change. Inoocyte yolk formation and growth, the number of its nuclear trophoblast cell nucleoli,lamphrush chromosome with a strong synthetic material; oocytes also some syntheticmaterial; follicle cells in the yolk protein synthesis, provide access to exogenous yolkprotein.

在卵子发生的整个过程中，卵母细胞、滋养细胞及滤泡细胞形态均有明显变化；在卵母细胞生长及卵黄形成期，滋养细胞核内的多核仁现象、灯刷染色体与旺盛的物质合成有关；卵母细胞本身也合成一些物质；滤泡细胞参与卵黄蛋白的合成，并为外源性卵黄蛋白提供通道。

In this article, the effect of different water pH (pH 5, 6, 7, 8, 9)on the secretion of nacre of freshwater pearl mussel Hyriopsis cumingii were studied with histochemical methods and transmission electron microscopy.

结果表明：在中性水环境中，贝体能积极地从外界水环境中吸收钙，并能旺盛地合成和分泌贝壳珍珠层及珍珠有机基质前体物质；持续的酸性水环境导致贝体的钙严重丢失，并引起珍珠质分泌细胞对有机基质前体物质的合成和分泌能力减弱；持续的碱性水环境虽能导致贝体对钙的积累，但珍珠质分泌细胞合成和分泌珍珠质有机基质前体物质的能力减弱；水环境的酸性或碱性程度越高，对珍珠质分泌细胞的合成和分泌活动的影响越大。

After working backwards in this way from the complex to the already known, it is possible to start building, synthesising, the molecule.

他自己还运用逆合成分析法，在试管里合成了100种重要天然物质，在这之前人们认为天然物质是不可能用人工来合成的。

The results implied that NO/ROS-induced ABA accumulation come from de novo synthesis pathway rather than degration of conjugated forms. The protein synthesis inhibitor actidone blocked the NO-induced ABA accumulation while having no effects on ROS-induced ABA accumulation, indicating that the action of NO was gene-expression dependent and that of ROS was gene-expression independent. These results suggested that NO may serve as a stress signal in the regulation of ABA-biosynthesis and ROS may serve as substrate in ABA biosynthesis or be involved in the regulation of enzyme activities.

1ABA合成抑制剂fluridone抑制ROS和NO诱导的ABA积累，表明NO／ROS诱导的ABA积累来源于ABA的从新合成而不是已有结合态ABA的代谢；(2)蛋白质合成抑制剂环己亚胺可以抑制NO诱导的ABA积累而对ROS诱导的ABA积累没有影响，说明NO的作用涉及基因表达而ROS的作用与基因表达无关，暗示NO的作用方式可能是作为一种干旱胁迫信号物质参与ABA合成的调节，而ROS则可能参与了相关酶活性的调节或直接参与了ABA合成的底物反应。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！