合成物

One has the ability of photo-cleavage of DNA and another one as potential anti-cancer drug has the ability of oxidative cleavage of DNA. 1. As for the first system, a new ligand----Tpphz is used as the intercalator of the targeted compound. The 〓 is synthesized and characterized by elemental analysis,〓H NMR, molar conductivity, AgNO〓 titration, UV and fluorescence spectra.

核酸的光切割方面，我们选择了近年来新发现的一个配体Tpphz作为目标化合物的插入配体，合成了新颖的〓单核配合物，并通过元素分析、〓H NMR、摩尔电导测定、中心离子滴定法、紫外光谱和荧光光谱对此化合物进行了详细的表征。

Part one (chapter 1~4) is about the coppermediated biomimetic oxidation of naphthols and 2-naphthylamine.

中文题名氧化还原酶模型及在仿生有机合成中的应用副题名外文题名 The model of oxidoreductases and its application in biomimetic organic synthesis 论文作者谭端明导师许遵乐教授学科专业有机化学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2002 论文页码总数172页关键词模拟酶氧化还原酶仿生氧化铜胺络合物抗肿瘤药物馆藏号BSLW /2003 /O629 /5 本文包括两部分内容：第一部分（1~4章）为萘酚类和2-萘胺化合物在含铜化合物作用下的仿生氧化偶合反应研究。

The title compound 〔WOS 3Ag 3Br(PPh 3) 3〕·(OPPh 3)was synthesized and the structure was determined by X ray diffraction.

报道了〔WOS3Ag3Br(PPh3) 3〕·(OPPh3)簇合物(C72 H6 0 Ag3BrO2 S3P4W ，Mr=176 4.6 1)单晶的合成和结构。

In this paper, a series of transition metal complexes were synthesized; interaction and reaction mechanism between complexes and DNA were studied.

本文在查阅大量文献的基础上，以1，10-邻菲咯啉和1，10-邻菲咯啉-5，6二酮为配体合成了一系列过渡金属配合物，详细研究了它们与pBR322DNA的相互作用及其机理。

The ligand was used to prepare for a series of its transition metal complexes by the replacement reaction with metal salts.

并用该杂原子大环配体与过渡金属的盐进行置换反应，合成了相应金属：Ni、Cu、Co、Zn和Cd的大环配合物。

Part II: Synthesis of chiral sulfinyl NADH models. Three synthetic routes are adopted, i.e.

第二部分：手性亚砜类辅酶NADH模型物的合成。

objective the aim of this study is to investigate the expression and the distribution of the nerve growth factor during the period of neural tube development of human embryo.method early development of neural tube was studied in human embryos about 35 gestational days by using immunocytochemical abc technique.result there were ngf immuno-positive substances in the cytoplasm and nuclei of neuroepithelial cells in the ventricular zones of neural tube.in the intermediate zone of neural tube,ngf immunoreactivity was detected in the nuclei of some neurons,or the processes of other neurons which contained no ngf-immunoreactive substances in their nuclei;the expression pattern of ngf in the marginal zone of neural tube was similar to that of the intermediate zone.the density of ngf-immunorecative particles was higher on the rostrum side of neural tube than on the caudal side.the ngf immuno-positive cells were also observed among the somites of embryo under the neural tube.conclusion these results suggest that ngf was an important signal molecule to induce neural tube differentiation,and that ngf may play a significant role in regulation of the biological function of neurons in developing neural tube.

目的 研究捷安肽素的抗真菌作用机理。方法采用形态学方法和同位素标记法。显微形态观察经捷安肽素处理后的供试真菌的形态学变化。进一步采用14c同位素标记的特异底物&尿苷二磷酸-（14c）-葡萄糖&示踪，研究捷安肽素对真菌(1，3)-β-d-葡聚糖合成酶活性反应的影响。结果研究神经生长因子在早期人胚神经管发育过程中的定位表达。方法采用免疫细胞化学 abc法染色，研究35天人胚的发育情况。结果在人胚神经管的室管带中，神经元的细胞质和细胞核ngf免疫反应阳性；在中间带，一部分神经元的细胞核ngf免疫反应阳性，另外一部分神经元的细胞核ngf免疫反应阴性，而其突起ngf免疫反应阳性；在边缘带ngf的表达与中间带相似。在神经管的头侧ngf阳性反应较强，神经管的尾侧ngf阳性反应较弱。结论 ngf在人胚神经管免疫反应阳性，表明ngf可能是诱导神经管分化发育的重要信号分子，提示ngf可能在人胚神经管的发育中具有十分重要的作用。神经生长因子；人胚；神经管；发育

The microstructure and phase constituents of sintered samples were studied by SEM and XRD.

用SEM和XRD研究了合成莫来石的显微结构和物相组成。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

It is very valuable that design and synthesize complexes as artificial cleavage regents of nucleic acid in theory and appliation.

设计和合成金属配合物作为人工核酸切割试剂具有非常重要的理论和应用价值。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失