合成物

Furthermore,enzymatic production of cephalexin using free penicillin G acylase in aqueous system wasstudied. The parameters, including pH, temperature, concentration of 7-ADCA and molar ratiosof 7-ADCA to phenylglycine methyl ester, that affect the yield of cephalexin, wereevaluated. In section Ⅱ, the integration technique of bioseparation and biosynthesis was investigatedfirst time in the enzymatic production of cephalexin.

第Ⅰ部分主要建立了对酶法合成头孢氨苄过程行之有效的HPLC检测方法，实现了头孢氨苄合成过程中所涉及的四种物质，即头孢氨苄、苯甘氨酸甲酯、苯甘氨酸及7-ADCA快速有效的检测；同时对水相体系中头孢氨苄合成过程的影响因素进行了研究，明确了水相体系中温度、pH值，底物浓度以及底物配比对酶法合成头孢氨苄得率的影响。

During the investigation of the reactivity of O_SCl_2(PPh_3)_2CH=C(PPh_3CHCH=CH_2 and O_SCl_2(PPh_3)_2CH=C(PPh_3C(=CH_2)CH=CH2, several stable osmacycles with novel structure, such as osmabenzenes, osmafurans, osmacycles containing intramolecular-coordinated allene, intramolecular-coordination osmacycles containing osmacarbene, an osmacyclopentadiene, were isolated during the investigation of the reactivity of O_SCl_2(PPh_3)_2CH=C(PPh_3CHCH=CH_2 and O_SCl_2(PPh_3)_2-CH=C(PPh_3C(=CH_2)CH=CH_2.In addition, some convenient and efficient methods to prepare these osmacycles were developed.

首先，合成出两种具有反应活性的金属杂五元环IC配合物OsCl_2(PPh_3)_2CH=C(PPh_3CHCH=CH_2和OsCl_2(PPh_3)_2CH=C(PPh_3C(=CH_2)-CH=CH_2，并对它们进行了系统表征；进而，研究了这些活性五元配合物的反应性，合成出多种稳定的锇杂环配合物，其中包括锇苯、锇呋喃、含配位联烯的锇杂IC环化合化物、含锇卡宾的IC环化物、首例锇杂环戊二烯化合物等多元化的产物。

The studies are significant to propose the synthetic methodology of benzothiazole derivatives,broaden the application of the metal complexes of benzothiazoles.It is practical value of the research in preparation of phenylacetic acid from metal-catalyized carbonylation.The thesis consists of four chapters.

本论文研究了苯并噻唑衍生物及其钴、铑金属配合物的合成，并研究了两类金属配合物的羰基化催化性能，提供了合成苯并噻唑衍生物的可行路线，拓宽了苯并噻唑金属配合物的应用范围，催化羰基化合成苯乙酸的研究具有重要的实用价值。

These compounds are of substantial interest both inviewpoints of theory and practical applications, mainlyunder the stimulus to achieve synthetic models either foractive sites of iron-sulfur proteins or for transitionmetal catalysts. This thesis describes the synthesis, structures andconformations of 51 new iron-sulfur cluster complexes,which have been characterized by elemental analysis andstandard spectroscopic methods. In addition, single-crystalX-ray diffraction analyses were performed for 4 representa-tive new compounds. Reactivities of μ-〓 with mono- and di- Grig-nard reagents derived intermediates toward various organicelectrophilic substrates were studied, along with complexa-tion of arynyl-containing iron-sulfur clusters by 〓Thus, several bridged, non-bridged, monomeric, dimeric,homonuclear, heteronuclear iron-sullfur and iron-sulfur co-balt-carbon cluster complexes were synthesized. A novelreaction and convenient route for the synthesis in highyield of tetranuclear iron complexes 〓 containing atetracoordinated sulfur atom was discovered, whereas aplausible mechanism based on experimental results was propo-sed for the formation of the new cluster type.

鉴于有机铁硫原子簇化学在基础理论发展和应用方面的重要意义，因此我们开展了这一课题的研究，并取得以下主要成果： 1、本文共合成了51个新有机铁硫及含钴碳簇基的有机铁硫原子簇配合物，除用元素分析，NMR，IR，MS法表征其结构和构象外，还用X-光衍射技术测得四个代表性配合物的单晶结构和构象； 2、我们业已发现炔基Grignard试剂同〓可形成一种&开环&与&闭环&中间物的平衡混合物，研究该平衡混合物同各种亲电试剂的反应； 3、研究发现了一种可高产率地形成〓含〓四配位硫挛合簇的新化学反应并对此反应提出了—种有趣的反应机理； 4、首次由1，4-二溴丁烷双Grignard试剂与〓制得一种新型铁硫簇双硫阴离子活性中间体，并进而研究了它的亲核反应性能； 5、找到了通过〓的光化学反应来合成簇合物〓-〓的一条有实用价值的新路线。

The aim of this study was to develop a novel amphiphilic functional block poly(ε-caprolactone) bearing ketone groups MPEG-b-P(OPD-co-CL. 2-oxepane-1, 5-dione was copolymerized with ε-caprolatone in which methoxy poly (MPEG, Mn=5 000) and stannous octoate were used as initiator and catalyst, respectively. The syntheses were conducted by bulk and solution polymerization. 1H NMR results showed that peak position and splitting of the polymers synthesized through solution polymerization were consistent with theoretical data, demonstrating the products were designed block copolymers. While in the bulk polymerization, polymer peak position was changed due to their high reaction temperature, suggesting their structural variation.

将带有羰基官能团的4-羰基己内酯和ε-己内酯分别在甲氧基聚乙二醇(MPEG，Mn=5 000)为引发剂，异辛酸亚锡为催化剂溶液和本体体系中开环聚合，合成了两亲性侧基带有羰基官能团的聚己内酯嵌段共聚物MPEG-b-P(OPD-co-CL.1H NMR结果表明采用溶液聚合法合成的聚合物各峰的峰位置和分裂情况与理论一致，证明了产物是实验所设计的嵌段共聚物，而采用本体聚合法合成的聚合物由于温度的影响，聚合物各峰的峰位置发生了变化，说明其结构发生了变化；差示扫描量热法分析结果表明随着OPD单体含量的增加，聚合物的熔点，玻璃化转变温度和熔融焓增加；热失重测试结果表明OPD单体的引入增加了聚合物的热敏感性。

By modifying the synthetic methods in literature,four multinuclear copper complexes,that is,dinuclear[Cu_2(H_2tea)_2(C_6H_5COO)_2]·2H_2O, trinuclear[Cu_3(H_2tea)_2(4-OC_6H_4COO)_2(H_2O)]·4H_2O(designated as Cu_2),tetranuclear O?

通过改进合成方法，从硝酸铜原料和三乙醇胺(标记为H_3tea)配体出发，合成了四种多核铜配合物，即双核铜配合物[Cu_2(H_2tea)_2(C_6H_5COO)_2]·2H_2O，三核铜配合物[Cu_3(H_2tea)_2(4-OC_6H_4COO)_2(H_2O)]·4H_2O(标记为Cu2)，四核铜配合物O？

This study is design and preparation of active peptide analogues of mung bean trypsin inhibitor Lys fragment, Trichosanthes trypsin inhibitor I, carcinoembryonic antigen peptide and aspartame using solid and liquid phase peptide synthesis methods.

本论文研究活性多肽类似物的设计、制备和功能，分为四个部分，采用多肽固相和液相合成的方法，分别合成了活性多肽绿豆胰蛋白酶抑制剂 Lys片段长链16肽、天花粉胰蛋白酶抑制剂、癌胚抗原肽、甜味肽等一系列类似物，并测定了这些类似物的生物活性，有以下新的研究结果：1、furin酶抑制剂的设计、制备和功能；2、芳香氨基酸芳环之间的π-π共轭能部分补偿二硫键的作用；3、癌胚抗原肽Gly4被L-Pro替代可增加与HLA-A2分子的结合；4、肽键对甜味肽保持甜味是必须的。

A New type of benzoxzine precursors was systhesized based on methoxy methylene diphenyl ether oligomer.The struture of product was characterized by IR and H-NMR analysis and effects of solvent polarity,temperature on the precursor composition were investigated.

以甲氧基次甲基二苯醚低聚物为基础合成了苯并口恶嗪预聚物，用H－NMR、IR表征了该预聚物的结构，讨论了反应温度、溶剂的极性等因素对合成预聚物的结构和组成的影响。

The photophysical property of the complex is investigated by means of IR spectra, Raman spectrum and laser excited, high-resolved luminescence spectra which indicates one kind of Eu site with local symmetry of C 2v in the complex.

合成了植物生长素对氯苯氧乙酸和 1，10 邻菲罗林与铕的三元配合物Eu 3·Phen 。对所合成化合物进行了元素分析和热谱分析。通过测定配合物的红外光谱、拉曼光谱和激光激发高分辨荧光光谱研究了配合物的光物理性质。荧光光谱表明配合物中Eu离子只具有一种化学环境，Eu格位的局部对称性是C2v

Part II. Synthesis, characterization and fluorescent properties of rare earthcomplexes of rutin Rare earth complexes of rutin have been synthesized by the reaction of rare earth chloride with rutin in alcohol solution (pH=7～8). The composition and thermal stability of the complexes were characterized by elemental analysis, molar conductivity, thermal analysis, IR, UV.

第二部分：稀土芦丁配合物的合成与表征及其荧光性质以稀土金属氯化物和芦丁为原料，在乙醇溶液中(pH=7～8)合成了6种稀土配合物，通过元素分析、IR、UV、热重-差热分析、摩尔电导率的测定对配合物进行了表征，确定了配合物的组成，分析了配合物的热稳定性和热分解行为。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失