合成物

Objective: To explore the effects of Gymnadenia conopsea alcohol extract on the collagen synthesis in rat lungs exposed to silica and the influence on antioxidase activities, level of lipid peroxidation.

目的：探讨手掌参醇提物(Gymnadenia conopsea alcohol extract， GcAE)对染矽尘大鼠胶原合成的干预作用及其对脂质过氧化(lipid peroxidation， LPO)水平、抗氧化物酶活性的影响。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

It is a key intermediate in the synthesis of material with nonlinear optical properties, functional molecular wires, polymetal heteronuclear complexes containing ferrocenylacetylide.

它是合成具有非线性光学材料、功能化的分子导线、多金属异双核配合物的关键中间体。

Therefore heteronuclear Cu-Ln compounds have commonly been synthesized with hetero-donor ligands comprising two types of ligating atoms coordinated each to Cu and Ln atoms, respectively.

因此通常用含有能分别和铜，稀土配位的两种配位原子的配体来合成异核Cu-Ln配合物。

In Chapter 3, six mixed-valence or heteronuclear metal imidazolates have been synthesized and structurally characterized through introducing univalent metal ion into bivalent metal imidazolates, which give a rich topological chemistry including novel topological networks.

其中尤为突出的是混价铜咪唑配合物的控制合成，通过精确调节反应条件得到了混价比例不同的产物，其中一种呈现出新型的四连接网络的拓扑类型。

CdS nanoclusters were synthesized by using the octylthioalcohol or dodecylthioalcohol as ligand and the hexadecyl trimethyl ammonium bromine as organic positive ion.

分别引入辛基硫醇和十二烷基硫醇作配体，十六烷基三甲基溴化铵作有机正离子合成CdS纳米簇合物。

Two kinds of high alcohol acrylates were prepared by octadecyl alcohol and hexadecyl branched alcohol,respectively.The polymers of depressants were prepared by maleic anhydride-styrene-higher alcohol acrylates.By using these depressants,the pour points of the Liaohe crude oils were lowered form 30℃ to 20℃.

分别采用碳十八直链醇、碳十六支链醇与丙烯酸合成烷基酯，将烷基酯再与顺丁烯二酸酐、苯乙烯聚合制备的三元共聚物，对辽河油田的高凝原油的降凝效果进行了评价，实验发现这两种聚合物对凝固点为30℃原油都具有较好的效果，降幅可达10℃左右，但对凝固点为41℃的原油较差分别为3℃和6℃。

A novel drum organooxotin cluster hexameric phenyloxotin 3 indolpropylcarboxylate was synthesized by the reaction of Ph 3SnOH with 3 indolpropylcarboxylic acid in 1∶1 molar ratio.

利用Ph3SnOH和 3 吲哚丁酸以 1∶1摩尔比反应，合成了新型含羧酸配体的鼓形有机锡氧簇合物：六聚苯基锡氧 3 吲哚丁酸酯。

To the degree that the astrologer identifies his horoscopical findings with the law of "as ye sow, so shall ye reap" will he be able to synthesize correctly the chart in its entirety, will he be able to reduce past causes of present conditions, will he be able to determine the potential solutions of difficulties.

作为占星家通过&种瓜得瓜种豆得豆&法则鉴别出的星盘上的发现物的角度，使他能够正确地合成整个星盘，使他能够还原导致现在条件的过去的原因，使他能够决定解决困难的可能的方法。

Central functional group ; hydroxamic acids ; cobalt complexes ; synthesis ; oxygenation

中心功能基；异羟肟酸；钴配合物；合成；氧合反应

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失