合成物

In the paper, hydrothermal synthesis of 4-4 'bipyridine and metal complexes.

本文采用水热合成法合成4-4'联吡啶与金属的配合物。

It was increased ethyl hexanoate synthesis substrate concentration mediated by Rhizopus chinensis 2113 whole-cell lipase after improved synthetic activity and optimized reaction condition. The reaction condition were: caproic acid concentration 2.4mol/L, substrate molar concentration ratio 1:1.1, initial water content ≤0.2%, lipase water activity 0.3~0.66, enzyme amount 16%, reaction temperature 30℃, agitation speed 150 r/min.

通过合成酶活的提高及反应条件的优化，提高了华根霉2113全细胞脂肪酶催化己酸乙酯合成的反应底物浓度，其反应条件为：己酸浓度为2.4mol/L；酸醇摩尔浓度比1：1.1；反应体系含水量≤0.2%；脂肪酶水活度0.3～0.66；加酶量16%；反应温度30℃；150r/min振荡反应。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

The article is focused on the current progress on the development of the transgenic crops accumulating PHB and cyanophycin by the gene engineering approach.The challenge and prospect of transgenic plant based production of biodegradable plastic was also discussed.

本文论述了应用基因工程，培育可合成PHB和蓝藻素等多聚物农作物的技术策略及研究进展，并讨论了培育合成生物降解塑料转基因植物所面临的挑战和发展前景。

The first asymmetric synthesis of trans-β-hydroxy-γ-butyrolactones:-(3R, 4R, 5S)-4-(1-acetoxy-isopropyl)-3-hydroxy-butyrolactone 65a was achieved starting from isobutylaldehyde through nine steps. The key steps involve the construction of three chiral carbon centers by the Sharpless's asymmetric epoxidation and Substrate directable chemical reaction.

以异丁醛为起始原料，经过九步反应首次设计合成了具有三个手性中心的trans-β-羟基-γ-丁内酯：-（3R，4〓，5S）-4-（1-乙酰氧基-异丙基）-3-羟基-丁内酯65a，关键步骤是Sharpless不对称环氧化和底物定向不对称合成反应。

In this chapter, A〓 is divinyl sulfone, B〓 organic molecules with two secondary-amino groups, BB'〓 organic molecules with one secondary-amino group and one primary-amino group.

提出了一种由已商品化的A〓、B〓和BB'〓型单体合成高度支化共聚物的新方法，并合成了六种新型高度支化共聚砜胺。

A satisfactory result was obtained in synthesis that using the material of high reactivity and high valence such as PPh3I*. The research area of material in electrosynthesis has been extended.

本文还重点研究了非水体系中不含酸性氢原料(I_2)的电化学反应机理，提出以电化学反应活性强的高价氧化态物质(如PPh_3I~+)作为阴极反应原料，在合成中取得了满意的结果，拓展了非水介质配合物电化学合成原料的研究和应用范围。

It is worthy pointing out that there are two types of Ag-C bonding models found in 19 including /7\'-C6H6-Ag and /72-Cp-Ag.Three new H-bonded one-dimensional supramolecular compounds, namely M(C3oH2iN2O2)2(C2H5OH)2 M - Cu(II (20), Co (21) and Mn (22), were also synthesized by the reactions of metal (Cu2+, Co2+, Mn2+) acetate with this new type of fulvene ligand.

用所合成刚性多齿有机配体与过渡金属Cu~(2+)(来源：AB4dC论文网www.abclunwen.com)、Co~(2+)、Mn~(2+)的醋酸盐反应，分别合成了含七员金属环的配合物(20—22)，在固态下，20—22为分子间氢键驱动的一维链状超分子聚集体。

Then two star-shaped oligomers with designed molecular weights 5000 g mol-1 and 10000 g mol-1, respectively, were synthesized from D, L-lactide and glycolide as raw materials and pentaerythritol as initiator under catalysis of dibutyltin oxide.

以合成的丙交酯和乙交酯为原料，季戊四醇为引发剂，氧化二丁基锡为催化剂合成出设计分子量为5000和10000两种预聚物。

By adopting the method of stepwise synthesis, this paper synthetizes 1,3 - diaminopropanein 2-Hydroxy-3-methoxybenzaldehyde Zn-Sm heteronuclear bimetallic in Anhydrous methanol solution.

6楼本文采用分步合成的方法，在无水甲醇溶液中合成了1，3-丙二胺缩邻香兰素Zn-Sm异核双金属配合物。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失