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To some degree, this study revealed the relationship between ossein and bone miner and the important preventive and therapeutic effects of ossein on OP. It provides theoretical and experimental basis for the research and development of effective prescriptions to cure OP. Limited by the actual experimental situation, we only did some study on the synthesis and content of type Ⅰ collagen. Further study on the changes of decomposition and structure of type Ⅰ collagen are needed to be done.

本课题的研究,在一定程度上揭示了骨胶原纤维和矿物质之间互根互用的辨证关系,及Ⅰ型胶原在骨质疏松症的发病和治疗中所起的重要作用,并为开发研制治疗本病的有效中药复方提供了理论和实验依据,但由于条件所限,本研究仅就Ⅰ型胶原的合成及其含量变化做了初步观察,而本病中骨胶原纤维的分解代谢及其结构的改变,尚待进一步探讨。

By the catalyzed of F3H, F3'H and F3'5'H, the biosynthesis of anthocyanins branched three pathway to form pelargonidin, cyanidin and delphinidin separately. DFR, F3'H and F3'5'H are the most important genes for introducing new metabolic branches by genetic transformation to plants that lack some kinds of flower pigments.

花色素苷的生物合成主要由于F3H,F3'H和F3'5'H三个关键酶的作用形成3个分支,最终分别生成橙色到砖红色的天竺葵素糖苷,红色的矢车菊素糖苷和蓝色到紫色的飞燕草素糖苷,因此, F3'H,F3'5'H和DFR基因是利用遗传转化引入花卉植物原本缺乏的花色代谢途径的关键基因。

In this study, the amount of ethylene and its key biosynthesis genes expression, typical allelochemical hydroxamic acid concentration and the key regulation genes (BX1 and BX9), as well as the dynamic expression and time of relevant defense key genes of secondary substance metabolism processes were compared in ethephon treatments of different concentration and duration.

为探明外源乙烯利对玉米化学防御作用诱导的浓度和时间效应,本文采用化学和分子生物学相结合的方法,研究了不同浓度乙烯利处理玉米后,叶片乙烯释放量及其合成调控关键基因ACS和ACO表达的变化,典型化感物质丁布的含量及其关键调控基因BX1和BX9表达的变化,以及其他调控次生代谢物的关键基因表达的时间动态。

The effects of several components in the culture medium on Lithospermum erythrorhizon cell growth and secondary metabolite synthesis were studied, as well as structural dynamic model. The two-liquid-phase culture of Lithospermum erythrorhizon was carried out by choosing the proper organic solvent as the second phase. The bioactive carrier for adsorption was prepared and the condition of cell immobilization was determined. We combined the technique of two-liquid-phase culture and immobilization to carry out the culture. We chose the suitable type of reactor, studied its characteristics and results of cell culture using this reactor. The fed-batch operation was also studied on the basis of twoliquid-phase culture and immobilization used in culture in the reactor.

本文研究了紫草细胞悬浮培养中培养基中多种成分对细胞生长与次生代谢产物合成的影响,进行了结构化的动力学模型研究;通过选择合适的有机溶剂对紫草细胞进行了双液相培养研究;通过确定以吸附为细胞的固定化方法,进行了生物活性吸附载体的制备与固定化细胞的制备研究;并结合双液相培养技术,对紫草细胞进行了固定化培养及其动力学模型的研究;对反应器进行选型,并进行冷模与热模研究;在反应器中进行了固定化紫草细胞的双液相培养条件下的流加操作研究。

Searching for specific ligand to a certain recepter or enzyme in human body from secondary metabolities of plants, animals and microorganisms, Optimization of lead compound under certain theory or hypothesis, and synthesis methodology investigation of pharmaceutically active compound are all tasks of medicinal chemistry.

药物化学的研究领域十分宽广,既有从动物、植物、微生物的次生代谢产物中寻找与人体内特异性的受体、酶的激动剂或拮抗剂,又有在理论、假想指导下的先导化合物的优化,以及生理活性化合物的合成方法学研究。

By the affinity chromatography,fusion protein is purificated. The antiserum of chicken and rabbit were prepared with the fusion protein and Ghrelin-KLH.With active immunity and passive immunity,confirmed that Ghrelin of chicken have no obviously impact on ultimum metabolite,plasma Insulin,glucagons, T_3 and T_4.It entrances plasma GH,but inhibit food intake and increase of fat and body weight.These conclusion are obviously different from the mammal.

以纯化的融合蛋白和人工合成的Ghrelin-KLH为抗原,制备了鸡、兔抗血清:通过主动免疫、被动免疫中和实验证实禽类Ghrelin对鸡血浆中代谢产物、胰岛素、胰高血糖素、甲状腺素浓度没有明显的影响,对GH的分泌有促进作用,但与哺乳动物中不同,Ghrelin抑制鸡的脂重、体重的增加,抑制鸡的摄食。

Itamin D from the skin and diet is metabolized in the lier to 25-hydroxyitamin D (Figure 1), which is used to determine a patient's itamin D status1,2,3,4; 25-hydroxyitamin D is metabolized in the kidneys by the enzyme 25-hydroxyitamin D-1-hydroxylase (CYP27B1) to its actie form, 1,25-dihydroxyitamin D.1,2,3,4 The renal production of 1,25-dihydroxyitamin D is tightly regulated by plasma parathyroid hormone leels and serum calcium and phosphorus leels.1,2,3,4 Fibroblast growth factor 23, secreted from the bone, causes the sodium–phosphate cotransporter to be internalized by the cells of the kidney and small intestine and also suppresses 1,25-dihydroxyitamin D synthesis.5 The efficiency of the absorption of renal calcium and of intestinal calcium and phosphorus is increased in the presence of 1,25-dihydroxyitamin D (Figure 1).2,3,6 It also induces the expression of the enzyme 25-hydroxyitamin D-24-hydroxylase (CYP24), which catabolizes both 25-hydroxyitamin D and 1,25-dihydroxyitamin D into biologically inactie, water-soluble calcitroic acid.2,3,4

从皮肤和食物来的维生素D在肝中代谢为25-羟基维生素D(图1),被用来决定病人体内维生素D情况的1,2,3,4;25-羟基维生素D在肾中被25-羟基维生素D1羟化酶(CYP27B1)转变为有活性的1,25-二羟基维生素D 。1,2,3,4由肾产生1,25-二羟基维生素D是被血浆甲状旁腺激素和血清钙,磷水平紧密调节。1,2,3,4由骨分泌的成纤维细胞生长因子23使钠磷协同转运蛋白被肾和小肠细胞内化及抑制1,25-二羟维生素D合成。5 在1,25-二羟基维生素D作用下肾和小肠吸收钙及磷的效率增高(图1)。2,3,6 它也包括25-羟四- 24 -羟化酶的表达(CYP24),且将1,25二羟基维生素D和25羟基维生素D异化成无生物活性,水溶性的维生素D3-23羧酸。2,3,4

Cultured cells lose the ability of DNA synthesis, mitosis, and finally the ability of cell proliferation after they have undergone a finite number of population doublings in vitro, though the cells still maintain the basic metabolic process.

体外连续培养的细胞在有限次数的细胞分裂后,丧失合成DNA及分裂的能力,最后导致增殖能力的丧失,但基本代谢过程仍能维持,这种现象称为复制衰老。

In experiment 2, pullets previously maintained on a folate-devoid diet for 4 weeks, were used to compare the bioavailability of PGA with natural tetrahydrofolate from digested bacterial cell powder after a single forced feeding for 4 weeks repletion.

试验二:利用新母鸡四周的叶酸耗竭与四周的补满试验,比较合成的氧化型喋醯麸胺酸(pteroylglutamic acid; PGA)与经消化的细菌细胞粉末(digested bacterial cell powder; DBCP,含有天然的还原型四氢叶酸),在新母鸡体内的吸收与代谢。

By splicing in genes from other bugs, the company was able to engineer synthetic metabolic pathways—ones that do not exist in nature—that enable the bacteria to produce isoprene from the sugars found in plant materials such as sugar cane, corn cobs and switchgrass, a tall-growing variety native to North America.

Genencor的科学家们通过将大肠杆菌的基因与其它菌类基因接合,则能研发出前所未有的代谢合成途径,使细菌能从植物所含的糖类中制造出异戊二烯,这些含糖植物包括甘蔗、玉米棒和北美特有的变异品种柳枝稷等。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。