合成代谢的

The cloning of corresponding biosynthetic gene clusters is essential for new drug discovery and yield improvement by metabolic engineering.

微生物产生众多结构和生物活性多样的次级代谢产物，其生物合成基因簇的克隆是药物创新和产量提高的必要前提。

Cloning of corresponding biosynthetic gene clusters is essential for new drug discovery and yield improvement by metabolic engineering.

微生物产生众多结构和生物活性多样的次级代谢产物，其生物合成基因簇的克隆是药物创新和产量提高的必要前提。

The antibacterial activities of most of fractions were similar to the pomegranate peel extract.(7) Respiratory inhibition experiment showed that the respiration of thallus was inhibited mainly through the hexose monophosphate pathway, which leaded to be short of NADPH, and induced metabolic function dysfunction. Growth curve assay showed that the pomegranate peel extract inhibited cytodieresis in logarithmic growth phase. After exposure to the pomegranate peel extract, bacterial protein was analyzed by SDS-PAGE. The results showed that protein synthesis was inhibited, especially for large molecular weight protein.

7呼吸抑制试验结果表明，石榴果皮提取物主要是通过抑制HMP途径，导致还原力NADPH缺乏，引起代谢功能障碍，达到抑菌的目的；加药组和正常组金黄色葡萄球菌生长曲线的对比结果表明，石榴果皮提取物能够抑制金黄色葡萄球菌对数生长期的菌体分裂；SDS-聚丙烯酰胺凝胶电泳分析表明，石榴果皮提取物能够抑制菌细胞蛋白合成，尤其对菌体内大分子量蛋白作用最明显。

The former stimulates the cells and transfer the signals into the cells through the ligation with receptors on the cell membrane. The latter, a derivative of nucleotide, causes a cytotoxic effect to suppress thymidylate synthetase activity, and, therefore, to damnify RNA and DNA synthesis.

前者通过与细胞表面的受体结合，把胞外刺激传递到胞内，诱导免疫受体介导的信号级联放大作用，引起一系列免疫效应；后者是是小分子的核苷酸衍生物，可直接进入细胞内，通过抑制胸苷酸合成酶及损伤RNA、DNA等对基因转录和核苷酸代谢产生影响，最后产生细胞毒效应。

The mechanism might be elevated the HIF-la in histocyte through which accelerate the expression of EPO level, enhance the body's ability of carrying and transporting oxygen and increase the activity of key enzymes in glycolysis in hypoxia mice, and elevated the ATP level to remission the energy metabolism in hypoxia mice.

马齿苋总黄酮能够提高小鼠抗缺氧能力，机制可能通过提高组织HIF-1α含量，促进EPO表达，增加了血红蛋白的合成和红细胞的数量，增强机体携运氧能力，同时促进了无氧酵解进行，缓解了缺氧小鼠的能量代谢障碍。

The mechanism might be elevated the HIF-la in histocyte through which accelerate the expression of EPO level, enhance the bodys ability of carrying and transporting oxygen and increase the activity of key enzymes in glycolysis in hypoxia mice, and elevated the ATP level to remission the energy metabolism in hypoxia mice.

马齿苋总黄酮能够提高小鼠抗缺氧能力，机制可能通过提高组织HIF-1α含量，促进EPO表达，增加了血红蛋白的合成和红细胞的数量，增强机体携运氧能力，同时促进了无氧酵解进行，缓解了缺氧小鼠的能量代谢障碍。

Immunohistochemistry technology was used to evaluated tyrosine hydroxylase of DA synthesis. DA, 5-hydroxytryptophan(5-HT) and their respective metabolic products 3,4 -dihydroxyphenylacetic, homovanillic are also precisely quantified in this research by using high-pressure liquid chromatography with electrochemical detector. The main metabolism monoamine oxidase of DA was processed too. RESULTS:WD can increase the synthesis and the metabolism process of DA .

本文观察了温胆汤及其配伍对大鼠刻板行为的影响，采用了免疫组化技术对多巴胺的合成酶—酪氨酸羟化酶进行了含量测定，应用高效液相-电化学法测定了DA、5-羟色胺（5-HT）及代谢产物3，4—二羟基苯乙酸、高香草酸的含量，并测定了DA主要降解酶—单胺氧化酶。

Cloning of cytosolic (GS1) cDNA and chloroplast glutamine synthetase (CS2) cDNA from Pisum satium was carried out previously in our laboratory.

谷氨酰胺合成酶(Glutamine synthetase， GS， E。 C。 6。 3。 1。 2)是植物氨同化过程中的关键酶，对植物的氮素吸收和代谢起着至关重要的作用。

Cloning of cytosolic (GS1) cDNA and chloroplast glutamine synthetase (GS2) cDNA from Pisum satium was carried out previously in our laboratory.

谷氨酰胺合成酶(Glutamine synthetase，GS，E.C.6.3.1.2)是植物氨同化过程中的关键酶，对植物的氮素吸收和代谢起着至关重要的作用。

It was a microsomal membrane-bound hemoprotein without known physiological functions at that time and was characterized by a

细胞色素P450具有加单氧酶的作用，并能参与机体解毒、甾体激素的合成、脂肪酸代谢等重要的生理反应，并广泛分布于动物、植物和低等真核生物中。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失