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合成代谢产物

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Essential fatty acids are important for cell membrane function, cholesterol metabolism and the synthesis of various metabolites.

必需脂肪酸是重要的细胞膜功能,胆固醇代谢和合成的各种代谢产物。

Nitric oxide is a freely diffusible,gaseous compound and an important signal molecule in living organisms. In plants,NO influences aspects of growth and development,as well as the ability of plant responses to stress.

一氧化氮(nitric oxide,NO)作为植物体一种新的信号分子,参与植物许多生理代谢的调节和次生代谢产物合成调控,而且可以作为胁迫响应的关键信使。

When 2.0 g/L of malate was added to the fermentation medium, the metabolic flux of byproducts L-Ala and lactic acid decreased by 22.1% and 16.5%. At the same time, the metabolic channeled to EMP and glyoxyl circle decreased by 2.26% and 9.09%. However, the metabolic flux channeled to HMP increased by 2.26%.The metabolic flux channeled to the L-glutamic acid synthesis pathway increased from 73.59% to 79.92%.

在L-谷氨酸发酵中、后期添加2.0 g/L苹果酸后,合成副产物L-丙氨酸和乳酸的代谢流量明显减少,分别降低了22.1%和16.5%,EMP途径和乙醛酸循环的代谢流分别减少了2.26%和9.09%,HMP途径的代谢流增加了2.26%,而L-谷氨酸生物合成的代谢流从73.59%增长至79.92%,较未添加前提高了6.33%。

Investigation of major metabolic pathways of SFZ-47 in animals The metabolites of SFZ-47 in rabbit urine following oral administration were separated and detected with HPLC and LC-MS methods. On the basis of the chromatographic behavior and mass spectra of the metabolites as well as biotransformation pathways of drugs with the partial structure of SFZ-47, the hydroxylic derivative 4-(3H-1, 2- dihydro-1-pyrrolizinone-2-methylamino benzyl alcohol and the carboxylic derivative 4-(3H-1, 2-dihydro-1-pyrrolizinone-2-methylamino benzoic acid of SFZ-47 were proposed as two potential metabolites and were synthesized.

在动物体内主要代谢途径的确定用HPLC和LC-MS对家兔口服SFZ-47后尿中的代谢物进行了分离、检测,根据代谢产物的色谱和质谱行为以及与SFZ-47有类似结构部分的其它药物的代谢途径,推测其羟基衍生物[4-(3H-1,2-二氢-1-吡咯里嗪酮-2-甲基胺基)苯甲醇]与羧基衍生物[4-(3H-1,2-二氢-1-吡咯里嗪酮-2-甲基胺基)苯甲酸]是两种可能的代谢产物,遂用化学方法合成了这两种衍生物。

Till now, Studies on Sorangium have mainly been carried out on its intriguing, novel and diverse bioactive metabolites and the genes for synthesis of these metabolites. However, to our knowledge no studies on the cellulolytic activity, the unique and basic property of Sorangium among myxobacteria have been reported yet.

目前国内外关于堆囊菌的研究大都仅仅局限于新颖、功能不同的活性代谢产物的筛选和鉴定以及代谢产物合成功能基因寻找和改造方面,而对于堆囊菌能降解纤维素大分子底物这一有别于其他粘细菌的自身独特的、最基本的特性,目前尚未见到相关的研究报道。

Firstly, based on phenomenal characteristic parameter collection and its further correlation analysis, the metabolic flux shift during late phase of guanosine fermentation was preliminarily deduced and confirmed by combining intermediates detection and the product biosynthesis pathway analysis. That is, the metabolic flux shifted from Hexose Monophosphate Pathway , from which guanosine was generated mainly, to Embden-Meyerhof Pathway and Tricarboxylic acid cycle to form amino acids and organic acids.

首先通过对发酵过程现象特征的收集和进一步的相关分析,初步推断在发酵过程后期存在有代谢流的迁移,结合中间代谢产物的分析和产物合成的代谢途径初步确认了代谢流迁移的存在,即代谢流从形成产物的HMP途径向形成有机酸、氨基酸的EMP途径、TCA循环迁移。

During the stationary phase of fermentation, cell productivity was slightly reduced. MgSO_4 and tryptone have the positive effect on the product synthesis and cell growth respectively. Based on these conclusions, MgSO_4 and tryptone were added to the feed medium to enhance the production of polysialic acid. Variable feed rate policies were applied to the fed-batch fermentation process and the final biomass and polysialic acid production were both increased by 40 percent.

通过摇瓶实验研究了大肠杆菌发酵过程中菌体生长和产物合成的影响因素,结果表明:产物合成与菌体生长是相耦联的过程,菌体生长受到菌体代谢产物的抑制作用,聚唾液酸的合成不受代谢产物的抑制作用;发酵进入稳定期后菌体的生长和生产能力有所降低;硫酸镁和胰蛋白胨分别对产物合成和菌体生长有一定的促进作用。

Artificial conditions of plant cell culture, such as plant growth regulators, nutritious factors and elicitors, can be as metabolic regulation signals to regulate plant cell growth and secondary metabolite biosynthesize.

植物细胞培养的人工环境中诸如植物生长调节剂、营养因子和刺激剂等代谢控制信号可以调节控制植物细胞生长和次级代谢产物的生物合成。

Determination of Tyrosine in Urine Spectrophotometric Method;2. For chemical pre-extraction, MSTFA/TMSL derivatization reaction, GC/MS analysis, it is confirmed that two main metabolites (19-no-randrosterone and 19-noretiocholanolone) were found in nandrolone urine.

本文对合成的19-去甲雄烷醇酮的四个差向异构体混合物进行气相色谱分离,对诺龙和炔诺酮用药后,留取的人尿样通过化学预处理,MSTFA/TMSI衍生化反应,GC/MS分析,确证了尿样中诺龙的两个主要代谢产物为19-去甲雄酮和19-去甲原胆烷醇酮,炔诺酮的两个主要代谢物是四氢炔诺酮的两个形式,可能是3α-OH-5α-THINE和3α-OH-5β-THINE,在炔诺酮代谢前期,还发现有诺龙的上述两个代谢物存在。

The basic concept is combining metabolic pathways in different organisms on a genetic level. As a consequence heterologous organisms provide precursors from their own primary and secondary metabolism that are metabolised to the desired secondary product due to the expression of foreign genes.

组合生物合成是得到新颖天然产物的一种新型工具,并且可以用于生产稀有珍贵的天然产物,其基本概念是在基因水平上将不同有机体的代谢途径结合起来,从而使异源性生物通过外源基因的表达代谢出期望的次生代谢产物,然后从它们的主要代谢和次生代谢中得到想要的前体。

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