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The leaves at physiological age of 20 days on the medium of MS+0.4 mg/L 6-BA+0.01 mg/L IBA reached the maximum inductivity of chimera buds,or 34.8%.

生理年龄为20 d的叶片,在MS+6-BA 0.4 mg/L+IBA0.01 mg/L 培养基中,嵌合体诱导率最大达到34.8%,暗培养会降低嵌合体的诱导率。

The superstructure has used the model of elastical constitutive relation; the pile-group foundation has quoted the soil-pile equivalent compound model. It has been analyzed the constitutive relation for soil-pile equivalent compound which regarded as the transversely isotropic body this construction relations, and determined the corresponding elastic constant.

上部结构采用线弹性本构模型,下部群桩基础引用了桩土等效复合体模型,分析了将桩土等效复合体视为横观各向同性体的本构关系,确定了相应的弹性常数。

In 42 ACME-arcA-positive isolates, 8 isolates harbored SCCmec V, 8 isolates harbored class C1 mec complex and ccrAB_, 22 isolates harboring class C1 mec complex and 4 isolates harboring class C2 mec complex were negative for all known ccr allotypes.

本研究在ACME-arcA基因阳性菌株中发现整合酶基因ccr的一种新亚型ccrAB_(SHP.42株ACME-arcA基因阳性菌株,8株携带V型SCCmec,8株携带C1型mec基因复合体和ccrAB_,剩余26株携带C型mec基因复合体和未分型ccr。

The results indicated that:The multi\|locus DNA fingerprint with a new synthesized probe\|JL\|02,has enough polymerism and good stability,and should be very useful to monitor the chimerism in different tissues of ES cell chimeric mouse and to check whether an ES cell line has the capacity to enter the germ line,especially when involving strains that can not be discerned with coat color or biochemical markers.

结果表明:采用新型的人工合成的寡核苷酸多聚体探针(JL-02探针)的多位点DNA指纹图谱,具有足够的多态性和很好的稳定性。适用于鉴定ES细胞在嵌合体小鼠各种组织器官中的嵌合情况,也适用于鉴定ES细胞是否具有种系嵌合能力,尤其适用于在嵌合体研究中,最适宜的品种组合具有相同的毛色或相同的酶型。

Seven experiments were conducted in the present study to test and verify two inferences:(1) In character decision performance, high frequency character have not character stroke and component number effect, but low frequency character is an essential condition to stroke and component number effect;(2) In prime condition, when SOA is very short (SOA=56ms), component character can prime the recognition of low frequency compound characters, but failure to prime the high frequency compound characters.

在此基础上,本文通过7个行为实验验证了这一假设的两个重要推论:(1)在字决定任务中,高频汉字不存在部件数和笔画数效应,而低频字不必然存在笔画数和部件数效应;(2)在启动条件下,当SOA较短(=56毫秒)时,成分字仅对低频合体字有启动效应,而对高频合体字没有启动效应。

At the same time, on the base of self-developing 3-D finite element models of craniofacial complex, this article explores the change rule of stress、 strain and displacement of craniofacial complex against different strength and direction of maxillary protraction.

同时在自主建立的颅面复合体三维有限元模型基础上,探讨了不同大小和方向的前牵引力对整个颅面复合体的应力、应变和位移变化规律,以期达到为临床力系统选择提供科学依据的目的。

The differential expression of NADH-ubiquinone oxidoreductase chain 4, one of those 13 subunits of subcomplex β, suggests that subcomplex β may have important function associated with pathophysiologic changs in reperfusion injury.

再灌注损伤后NADH-泛醌氧化酶复合体4链的表达提示原来功能不明的NADH-泛醌氧化酶复合体β亚基可能在这一病理过程中发挥一定的作用。

The regulation of gene expression is to have relations to the assembly of the DNA binding proteins which bind to DNA to initiate the transcription. Some of these proteins have the ability to promote bending or looping of DNA. The FIS protein regulates gene expression in Escherichia coli by this property of bending DNA. There are many researches to discover the mechanism of FIS protein binds to DNA. However the experiment data only show the supposititious results and figure out the model of FIS-DNA complex structure. This research uses molecular dynamics simulation to compute the detail of the interaction between FIS and DNA. We observed FIS prominently affects the DNA bending by the type of local bending. Analyzing the DNA structure properties, roll and slide, also demonstrates the local bending type and region. The hydrogen bonds between FIS and DNA are analyzed to compare with the experiment data. Finally we compute the binding free energy between FIS and DNA to estimate the result of simulation and compare with the value of λ-repressor-DNA complex.

基因的表现与结合於DNA促使DNA转录作用起始的DNA结合蛋白组装有关,一些这类的蛋白质具有促使DNA扭曲或将DNA形成环状的能力,FIS蛋白质即是藉由扭曲DNA的特性调控大肠杆菌基因的表现,目前已有很多的研究发现FIS与DNA结合的机转,然而这些实验的数据仅能显示推测性的结果,进而利用这些结果推论出FIS与DNA聚合体结构的模型,本研究利用分子动态模拟的方式计算FIS与DNA交互作用的细节,我们观察到FIS主要影响DNA的扭曲方式为DNA区域扭曲的类型,并且分析DNA结构特性roll与slide也指出扭曲方式为区域扭曲与其扭曲的区域,我们也分析FIS与DNA之间的氢键,并且与实验的资料作比较,最后我们计算FIS与DNA之间的结合自由能评估模拟的结果,并且计算出的值和λ-repressor与DNA聚合体结构的实验值作比较。

On the functional differentiation,we found and that the type II ras-GTPaseactivating protein (p100-GAP),which was specifically expressed in humanplacenta,was largely expressed in the highly-differentiated syncytiotrophoblastcells and moderately expressed in the cultured intermediate trophoblast cells.While in the non-differentiated cytotrophoblast cell line-NPC,no expression was observed.Its mRNA and protein expressingcharacterization was in consistent with that of hCGβ,which was one of the mostimportant markers of the trophoblast cell functional differentiation.In addition,the expressing amount of p100-GAP increased with the progressing of pregnancyand the syncytium formation in vitro.

细胞功能分化的研究结果表明:经重组质粒的构建获得特异表达于人胎盘的p100-GAP的cDNA探针后,从蛋白和分子水平证实p100-GAP大量表达于分化程度高的合体滋养层细胞,少量表达于体外培养的中间型滋养层细胞,而非分化的细胞滋养层细胞NPC则不表达,这种表达特性与作为滋养层细胞功能分化重要指标之一的hCGβ高度一致;p100-GAP表达量还随妊娠过程和体外合体化过程而逐渐增加。

Abstract] Objective To observe the clinical effect with Dyrat complex for 364 cases of wedge-shaped defect.

目的 通过对364例楔状缺损应用光固化复合体充填的长期观察,追踪观察复合体的临床效果。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。