号外

The evening paper has an extra today.

这份晚报今天有号外。

We cannot always have associative law for operations, for instance, we did not have this law for mixed addition and multiplication,neither for subtraction, 2+3 2+1≠(2+3)(2+1)=5 3,（2-3）- 2≠2-（3-2）,this is why we have to set the rule in operations that "doing inside bracket at first and out side afterwards, doing multiplication and dividing first and addition and subtraction afterwards".

注意运算的结合律并不是永远对的，比如加法和乘法的混合运算就没有结合律，减法也没有，2+3 2+1≠5 3，（2-3）- 2≠2-（3-2），因此我们运算中总是规定先括号内再括号外，先加减后乘除。

PCR-SSCP was used to screen exons in 45 Wilson disease patients from 39 Chinese families and in 10 normal controls. Nucleotide sequence analysis by autoradiograph and restriction enzyme digest were used to detect the mutant points.

对中国人WD39个家系45例患者的3～20号外显子进行聚合酶链反应-单链构象多态性分析，对有异常者进行序列分析，并通过对该突变点的酶切再次筛选。

①Location of WD gene in Ch inese: Using pairwise linkage analysis and multipoint linkage analysis method, w e constructed a genetic map of DNA markers within D13q14.2-3 which refined the location of WD gene by restriction fragment length polymorphism and microsatellite polymorphism analysis;②Screen for mutations of WD gene in Chinese people: we detected the structure of 21 exons of WD ge ne in 45 patients from 39 pedigrees by PCR-SSCP(Single strand conformation poly morphism) and PCR-DNA sequencing technology, found a new mutation in exon 5 and nuclcotide sequence analysis showed it is a T insertion. We also conformed the Arg778Leu in exon 8, the highest frequence mutation point in Chinese people, wit h mutation rate 22.8%in total;③Carrier detection and presymptomatic diagnosi s of WD: Based on DNA recombination technology, we peformed successfully the gen e diagnosis in all individuals of 79 families with WD and built up a helpful spe cific enzyme cut method (PCR-Msp1) to detect the carrier and presympomatic patients in Chinese pe ople with WD.

①WD的基因定位研究：通过RFLP及微卫星多态性分析，应用两位点及多位点连锁软件，建立了中国人WD基因在D13q14.2-3区域的精细遗传连锁图谱，从而首次对中国人WD基因进行了精确定位；②WD基因突变研究：应用PCR-SSCP及DNA测序技术，对39个家系45名WD患者进行该致病基因的21个外显子突变筛选，发现WD基因5号外显子存在新的T插入突变，并证实中国人WD基因的突变热点为8号外显子，突变形式为Arg778Leu，其频率为22.8%；③WD的症状前诊断和杂合子检出：应用DNA重组技术对79个家系进行基因诊断，成功地进行了WD的症状前诊断和杂合子检出，并建立了WD的基因筛选的PCR-Msp 1酶切方法。

To discuss the polymorphism of cytidine deaminase from patients with acute leukemia and the patients curative effect.

目的 探讨急性白血病病人CDA基因第1号外显子基因多态分布以及追踪随访不同基因型患者的疗效与转归。

Among them, mutations in exon 7 was highest, while in exon 5 was lowest.

其中以第7号外显子出现的频率最高，第6、8外显子次之，第5外显子最低。

Objective To analyze the mutation characterization in exon 8 of ATP7B gene in Chinese Patients with Wilson disease.

目的 分析肝豆状核变性ATP7B基因8号外显子在中国人中的突变特点。

Objective To analyze the frequency of mutation in exon 8 of Wilsons disease gene in Chinese people.

目的 对中国人WD基因8号外显子进行突变分析。

For genotyping of SNPs direct sequencing was performed in 96 patients with EH and 96 normotensive controls.

结果 在胰高血糖素基因的 2号外显子(36 89位)和 3号外显子连接区(5 5 0 5位)各检测到 1个SNP。

One transcript contains exon 3 and 4, the second contains exons 1, 3 and 4 and the third contains all four exons.

其中一个转录物包含第3、4号外显子，第二个包含第1、3、4号外显子，第三个转录物包含有全部4个外显子。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。