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叶绿素

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We selected copper chlorophyll and copper sodium chlorophyllin to have experiments of culturing microalga, under the same of illumination and the concentration of nutrition, comparing with copper ion. The results show that under the condition of illumination, a certain quantity of photosensitizer can produce a great quantity of active oxygen, which has strong effect of death; Copper chlorophyll and copper sodium chlorophyllin have very strong influence to growth of microalga.

由于叶绿素铜钠是水溶的,而叶绿素铜是油溶的,导致微藻对它们吸附性质的不同,微藻吸附的少量叶绿素铜钠促进了微藻的生长,而叶绿素铜在低浓度时对微藻的生长具有促进作用,在高浓度时可以抑制微藻生长、甚至杀灭微藻。

Chlorophyll a, c2 and c1 and the carotenoids fucoxanthin, diadinoxanthin, diatoxanthin and β,β-carotene were existed in all species. No new marker pigment was found in these species.

叶绿素中的叶绿素a、叶绿素c2、叶绿素c1和类胡萝卜素中的岩藻黄素、硅甲藻黄素、硅藻黄素和β,β-胡萝卜素存在于所有的种类中。

Content of Chla,β-carotene and zeaxanthin of S. platensis grown in Se decreased, violaxathin, fucoxanthin, 19'-hexanoyloxyfucoxanthin and peridinin increased, Chla and Chlb of D. salina also decreased, but carotene increased, all pigments of D. zhanjiangensis increased except violaxanthin and 19'-hexanoyloxyfucoxanthin decreased. Effect of Ge on pigments of microalgaes were different in different algaes: Chla,β-carotene and zeaxanthin of S. platensis decreased, three other carotene increased, in D. salina, violaxanthin and lutein decreased, violaxathin, fucoxanthin and 19'-hexanoyloxyfucoxanthin increased, all pigments of D. zhanjiangensis increased except fucoxanthin and peridinin decreased; Chla,β-carotene and violaxanthin of Chlorella sp increased, but fucoxanthin and peridinin decreased.

暴露于硒中的钝顶螺旋藻(S.platensis)的叶绿素A、β-胡萝卜素和玉米黄素的含量都减少,岩藻黄素、19'-乙酰氧化岩藻黄素、多甲藻素和紫黄素的含量明显增加,盐生杜氏藻的叶绿素类也减少,但类胡萝卜素的含量则增加,湛江叉鞭金藻(D.zhanjiangensis)中除紫黄素和19'-乙酰氧化岩藻黄素有减少外,其它色素都增加;锗对四种微藻光合色素的影响也不尽相同,钝顶螺旋藻(S.platensis)的叶绿素A、β-胡萝卜素及玉米黄素含量减少,岩藻黄素、19'-乙酰氧化岩藻黄素和紫黄素的含量增加,盐生杜氏藻中除紫黄素和叶黄素有增加外,其它色素都减少,湛江又鞭金藻(D.zhanjiangensis)正相反,除岩藻黄素和多甲藻素有减少外,其它色素都增加,小球藻的叶绿素A、β-胡萝卜素和紫黄素增加较大,但岩藻黄素和多甲藻素则减少。

As for chlorophyll, rice and potato have the highest chlorophyll concentration, the value is 25%~35%; the next are corn and sugar cane with value of 20%~30%, and chlorophyll concentration of chestnut is 20%~25%.

对于叶绿素,水稻、马铃薯的叶绿素浓度最高,为25%~35%,其次为玉米、甘蔗,叶绿素浓度为20%~30%,而栗树的叶绿素浓度为20%~25%。

The Value of Ci of leaf in Rain cultivation is less than in Open-air cultivation.The content of chlorophyl a and b of Rosario Bianco leaf in Rain cultivation is higher than in Open-air cultivation and the chlorophyl content of the 5th leaf is maximal in Rain cultivation. Stem diameter of Rosario Bianco were increased 8.68%~10.74% during the stage of rain growth in Rain cultivation. The study shows the leaf area of Rosario Bianco in greenhouse is lager than unsheltered and the environment of greenhouse is suit for grape leaf growing.

避雨棚对葡萄叶片的胞间二氧化碳浓度有抑制作用;避雨棚对'白罗莎里奥'叶片叶绿素a和叶绿素b含量的影响显著:避雨栽培条件0000下叶片的叶绿素均高于露天栽培,叶位5的叶片叶绿素含量最高;避雨栽培模式较露天栽培在避雨阶段葡萄茎粗增加了8.68%~10.74%且避雨栽培可显著增加葡萄叶面积。

The results were as follows: 1. Response of physiology indices of Medicago falcata L. to drought stress is different. There are not obvious correlation among indices and ability to drought resistance. The indices include critical water saturation deficiency, free and bound water content, tissue water, peroxidase isozyme, free proline content, chlorophyll a, chlorophyll b, total chlorophyll and chlorophyll. These indices are not fitting for assessing ability of drought resistance of Medicago falcata L..

黄花苜蓿各生理指标对干旱胁迫的响应不同,临界饱和亏、自由水含量、束缚水含量、组织水含量、过氧化物同工酶、游离脯氨酸含量、叶绿素a、叶绿素b、叶绿素总量以及叶绿素与黄花苜蓿抗旱能力之间不存在明显的相关性,不适宜采用这些指标来评价或判别黄花苜蓿材料间抗旱能力的强弱。

The growth of H. fusiformis was determined by weighting the fresh algae every two days and the photosynthetic oxygen evolution was assayed by a Clark-type oxygen electrode. To determine the contents of chlorophyll a and UV-absorbing compounds, methanol extracts from H. fusiformis were scanned from 250 to 750 nm. The concentration of chlorophyll a was calculated using the equation of Porra and the contents of UVAC per fresh mass were estimated by determining the ratio of UVAC to chlorophyll a according to Dunlap et al and multiplying the ratio of chlorophyll a content to fresh weight of the sample used.

羊栖菜的生长是通过每两天测量一次藻体的湿重来测定的,光合放氧是用Clark型氧电极测定的,为了测定藻体叶绿素a和紫外吸收物质的含量,从250 nm到750 nm对羊栖菜的甲醇提取液进行扫描,叶绿素a的浓度用Porra的公式计算,紫外吸收物质的计算是根据Dunlap的方法先计算紫外吸收物质和叶绿素a的比率,然后乘以每单位藻体叶绿素a的含量。

Accordingly, the Q bands of them blue shifted by 6mn and 13nm, respectively. The fluorescence emission wavelengths of Cd-pheophytin a and Cd-pheophytin b observed at 660nm and 649nm, respectively, which were shorter than those of corresponding pheophytin a and pheophytin b by 10nm and 9nm, respectively.

研究发现:在光吸收性质上,与脱镁叶绿素a和b相比,镉叶绿素a和b在Soret带的吸收峰位置分别红移了19nm和11nm,而在Q带则分别蓝移了6nm和13nm;在荧光性质上,镉叶绿素a和b的荧光发射波长比脱镁叶绿素a和b分别蓝移了10nm和9nm。

Successful separation of Cd-pheophytin a, Cd-pheophytin b, chlorophyll a, chlorophyll b, pheophytin a and pheophytin b in 14min was firstly accomplished by reversed-phase HPLC at a flow rate of 0.8ml/min, with the mobile phase of methanol: acetone (80: 20, v/v).

首次以甲醇:丙酮=80:20为流动相,流速为0.8ml/min,在C〓柱(5μm,4.0×250mm)上,14min内成功实现了镉叶绿素a和镉叶绿素b配合物与叶绿素a,b和脱镁叶绿素a,b的高效液相色谱分离。

Oneyear old poted Fraxinus velutina and Robinia pseudoacacia seedings were tested by neutral salt and mixed salt solutions.The dynamic changes of a,b and total contents of chlorophyll were studied and the correlation of chlorophyll with MDA and Pro was analysed.

用不同浓度的中性盐和碱性盐(NaCl、NaHCO3、Na2SO4、 Na2CO3)溶液处理1 a生刺槐和绒毛白蜡,研究了两树种叶片中叶绿素a、叶绿素b和总叶绿素含量的动态变化以及叶绿素和丙二醛、脯氨酸的相关性。

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