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叶绿体

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The result showed that twocDNA fragments which expressed at high level both in shoot and radicle representedthe gene encoding beta-D-glucosidase; one cDNA fragment expressed specifically inshoot represented the gene encoding mitochondria HSP60; most clones of MF12 andMF17 fragments respectively represented the chloroplast genes encoding prp22 andprp19 proteins which are two components of ribosomal small subunit; while thededuced amino acid sequence from each exceptional one clone was respectivelyhomologous to CDC5 proteins and vesicle-associated membrane proteins; otherthree cDNA fragments expressed preferentially in shoot had no homologue inGenBank.

结果发现2个在胚芽和胚根中表达量都很高的cDNA片段代表的是编码玉米β-D-葡糖苷酶的基因;一个在胚芽中表达而在胚根中不表达的片段代表的是编码线粒体分子伴侣HSP60的基因;片段MFl2的大部分克隆测序结果是与叶绿体基因组中编码核糖体小亚基蛋白prp22的基因同源,但其中有一个克隆测定的cDNA片段序列推测的氨基酸序列与CDC5家族成员有较高的同源性;片段MF17的大部分克隆测序结果与叶绿体基因组中编码核糖体蛋白prp19的基因同源,而有一克隆测定的cDNA片段序列推测的氨基酸序列与参与信号转导的膜结合蛋白VAP27和VAMP有较高的同源性;另有3个优先在胚芽中表达的cDNA片段未查询到同源序列。

Above all, the NtFtsZ2-l protein in Nicotiana tabacum taked part in the process of chloroplast division, but in transgenic plants perhaps it had no obvious effect on the normal function of chloroplasts.

综上所述,烟草中NtFtsZ2-1蛋白参与了叶绿体的分裂过程,但可能并未对转化植株叶绿体的正常功能造成明显的影响。

With increasing of salinity , there were no obvious difference in quantity and shape of chloroplast under SEM , but their location in palisade cells occurred change .

扫描电镜下栅栏组织细胞中的叶绿体数量和形态未见明显的变化,但叶绿体在细胞中的位置发生了改变。

The thickness of palisade tissue decreased, and that of spongy tissue had no relation with salinity, the ratio of the thickness of palisade tissue to spongy tissue decreased with increasing salinity.

扫描电镜下栅栏组织细胞中的叶绿体数量和形态未见明显的变化,但叶绿体在细胞中的位置发生了改变。

This paper summarizes the appropriate range of some DNA sequences that are commonly used in current pteridophyte molecular phylogenetics, which comprises sequences from three plant genomes (chloroplast, nucleo-tide, and mitochondria), especially focuses on the usage of chloroplast DNA regions.

本文综述了目前蕨类分子系统发育研究中常用的DNA序列分析,它们分别来自叶绿体基因组、核基因组和线粒体基因组,着重阐明叶绿体基因在蕨类分子系统学研究中的应用。

Therefore, the both affected ion exchange and changed ion balance inside and outside of chloroplast membrane. ABA reduced pH of chloroplast suspension and dropped electrical potential of chloroplast membrane, ZT increased this electrical potential.

因此,二者可影响叶绿体膜内外离子的交换,改变膜内外质子的平衡,ABA降低光下叶绿体悬浮介质的pH,降低膜的电势值,ZT则增加其电势。

Grew on the normal temperature, it was shown that the deposits of calcium antimonate being the indicator for Ca(superscript 2+) localization mainly concentrated within the vacuoles and intercellular spaces and there was also some Ca(superscript 2+) deposits in cell walls. But when Garyota urens L. was treated by the temperature of 2℃ for 48 h, the level of Ca(superscript 2+) increased in cytoplasm and plasma membrane, but decreased in vacuoles and intercellular spaces considerably. At the same time, the ultrastructure of chloroplasts suffered from chilling: the membrane of chloroplasts had been damaged, the layer of thylakoids was exiguous and unclear, the photosynthetic rate decreased evidently. And when Garyota urens L. was treated by the temperature of 2℃ for 120 h, the deposits of Ca(superscript 2+) mainly concentrated within the cytoplasm, nucleus and plasma membrane and there was also some Ca(superscript 2+) deposits in vacuoles, and the ultrastructure of some cells was simultaneously damaged severely: Chloroplasts structure, vacuole membrane and nuclear membrane had been damaged fully, the structure within the cell had become unclear, and the cell only have respiration.

研究结果表明,未经低温处理的董棕幼苗叶肉细胞,焦锑酸钙沉淀颗粒大量出现在液泡和细胞间隙中,细胞壁中也可见少量沉淀,而细胞基质中则看不到焦锑酸钙沉淀;经2℃ 48 h低温处理后,细胞基质和细胞膜上焦锑酸钙沉淀增加,而液泡和细胞间隙中的焦锑酸钙沉淀则显著减少,并且超微结构已初步显示出寒害的特徵,叶绿体外膜部分破损,类囊体片层稀疏且排列不规则,光合速率明显下降等;经2℃ 120 h低温处理后,细胞间隙内的焦锑酸钙沉淀极少,有的也紧贴在细胞外壁上,而细胞基质和细胞膜上则分布有非常多的焦锑酸钙沉淀,在核基质和液泡中也可见到少量的焦锑酸钙沉淀,并且超微结构遭到了显著破坏,叶绿体结构完全被破坏,核膜与液泡膜严重破损,内部结构模糊,细胞只表现为呼吸作用,不进行光合作用。

The CO2 saturation point and light saturation point of B ascended and CO2 assimilatory ability, efficienecy of light energy utilization and temperature effect increased.

低钾处理初期,B叶绿体基粒数多于A,并且片层密度加厚;低钾处理后期,B与A叶绿体结构与形态趋向一致。B的CO2饱和点上升、光饱和点上升,CO2同化能力增加及光能利用率、温度效应增加。

The upper and lower epidermal in the yellow leaf of Celtis julianae 'Aurea' had almost no chloroplast, but after reverting green the chloroplast appeared and the number of the chloroplast, starch grains increased and plastoglobulus reduced.

金叶珊瑚朴黄色叶的上下表皮细胞中几乎不含叶绿体,但转绿后的叶片中上下表皮叶绿体的数目增多,故叶色变绿。叶色由黄转绿过程中,淀粉粒增多,体积变大,嗜锇小球减少。

The results showed that plant height, branching number, dry matter weight and uptake of B and Mo of Chamaecrista rotundifolia were increased by application of 1.0, 3.0, 7.0 mgkg^(-1) of boric acid or 0.5, 1.0, 4.0 mgkg^(-1) ammonium molybdate, the stabilities of cell wall, cell membrane and chloroplast membrane structure enhanced, the number of chloroplast increased, and the best effects were observed by the application of 3.0 mgkg^(-1) boric acid or 4.0 mgkg^(-1) ammonium molybdate.

结果表明,施用1.0、3.0、7.0mgkg^(-1)的硼酸或0.5、1.0、4.0mgkg^(-1)的钼酸铵都能增加圆叶决明的株高、分枝数、干物质重和硼/钼元素的吸收,稳定细胞壁、细胞膜、叶绿体膜的结构,增加叶绿体的数量;其中以3.0mgkg^(-1)的硼酸或4.0mgkg^(-1)的钼酸铵为最佳施用量。

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