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Keywords Cucumber;heat tolerance;heat injury symptoms;cDNA-AFLP;transcription repair coupling factor - TRCF;protective enzyme;HSP70 gene of the chloroplastic gene

Article From 南京农业大学;蔬菜学博士论文 2007年度黄瓜;耐热;热害症状; cDNA-AFLP;转录修复偶联因子;保护酶;叶绿体基质HSP70基因

The result of the leaf anotomical structure, the chloroplast pigment contents and the photosynthetic characteristics can provide diagnosis and correction of the iron deficency, according to the degree of chlorotic leaf The regreening effect of iron compounds, concentration, pH value and mixture more compounds were stud...

对不同程度黄化叶片的解剖结构、叶绿体色素和光合特性的分析为缺铁黄化诊断和矫治提供依据。

In addition,the leaves of the control plants appeared to be much reder than the transgenic tomato,and the change were obvious followed by far from the treated time at low temperature,which suggested that the constituently expression of the chloroplast sHSP had some protective fountions to the tomato at low temperature.2 Effect on contents of MDAMDA is the product that membrane lipid peroxide and the contents of MDA in plants can reflect the degree of the damage that the plants are encountered to some extent.

低温处理6周后,转基因苗叶片中chla,cib含量及叶绿素总量明显高于对照,反应叶绿体中类囊体垛叠程度的chla/the比值也大于对照,可见冷害条件下转基因植株体内叶绿素的破坏程度小。4))t%hs用:低温Al:Pll6周,测定植物光合作用的重要指标净光合速率、蒸腾速率、气孔导度、胞间隙CQZ鹏,结果显示赂因番茄的即雏率、蒸腾速率、气孔导度均明显高于对照,而胞间隙COZ 氏于对照。

This phenomenon warrants further study. 4. Phylogeny study on Croomia and Stemona The relationship of the genus and the taxonomic placement of Stemonaceae The phylogenetic tree of matK, trnL-F, atpB-rbcL and ITS sequences data indicated that it was natural to place Stemona and Croomia in one family.

黄精叶钩吻属和百部属的分子系统学研究百部科属间关系和科的系统地位叶绿体DNA(matK、trnL-F和atpB-rbcL)及核DNA序列构建的系统树显示Stemona和Croomia形成稳定的单系类群,表明将这两个属放在同一个科是自然的,不支持将Croomia独立成科。

The main results are listed as following: 1. Phylogeographic study on Croomia The relationships and distribution of cpDNA haplotypes suggested that Croomia had been widely distributed in ancient eastern Asian and northern American; the origin and distribution center of Croomia were concentrated on the mountains comprised Mt.

Croomia亲缘地理学根据叶绿体DNA(trnL-F、atpB-rbcL)单倍型的关系和分布推测,Croomia在古欧亚大陆和古北美大陆有着较广泛的分布;我国的黄山和天目山一带是Croomia的分布、起源中心,其散布路线是从中国大陆到日本,再到美洲古大陆;北美种群与东亚种群分离较早;东亚种的分化时间比较晚。

In order to study the degradation of protein and changes of endopeptidase in cucumber Cucumis sativus L.

因此叶片蛋白质的降解可能与叶绿体结构的变化和叶片内的氧化胁迫增加有关。

In this work, we used the sequences of nuclear genes (ITS, Adh2, Adh3 and Waxy) and cpDNA (trnL-F and matK) to explore the origin of Elymus species and the Y genome, and to reveal the mechanism of the geographical differentiation of the StY-genome Elymus species.

本文通过核基因ITS、Adh和Waxy基因以及叶绿体基因trnL-F和marK的序列分析了披碱草属相关二倍体种的系统发育关系和披碱草属植物的起源。

The phylogenetic relationships of Fagales were investigated by means of molecular systematics, which was based on six DNA sequences from three genomes, including chloroplast DNA trnL-F, matK, rbcL, and atpB, nuclear 18S ribosomal DNA, and mitochondrial gene matR.

本文采用来自三个基因组--叶绿体、线粒体和核基因组的六个DNA序列片段,对壳斗目的系统发育进行了重建,主要探讨了壳斗目八个科的科间系统演化关系及不同基因系统树间存在冲突的原因。

The results were summarized as follows:1 Four cpDNA trnL-F, matK, rbcL, and atpB sequences analysis The chloroplast trnL-F, matK, rbcL, and atpB sequences from 31 species representing nearly all genera of Fagales (sensu APG, 2003) were used to reconstruct the phylogeny of the order.

主要内容包括:1 基于四个叶绿体DNA序列的分析以金缕梅属Hamamelis和朴树属Celtis为外类群,对壳斗目几乎所有属的代表共31种植物的trnL-F、matK、rbcL和atpB序列进行了测定,通过四个序列单独和联合分析,得到如下结果:除rbcL基因树由于信息位点少而分辨率较低之外,壳斗目各科作为单系类群在各种分析中都得到了较强的bootstrap支持。

With the aid of PDQuest 2-DE software, 11 significant protein dots were found out and then 9 of them are pick out to go through peptide mass fingerprinting based on MALDI TOF MS and NCBI database searching . In all, 5 proteins were identified: Ribulose bisphosphate carboxylase , Nucleic acid-binding protein , Ribosomal protection tetracycline resistance protein , hypothetical protein FG04726.1 [Gibberella zeae PH-1], nitric oxide synthase .

应用PMF技术对这11个具有显著表达差异蛋白点中的9个进行鉴定,得到了5个质谱阳性鉴定结果,其中2个蛋白分别为玉米种属的1,5-二磷酸核酮糖羧化酶/加氧酶和叶绿体的核酸结合蛋白;其它的3个蛋白在玉米蛋白质数据库中未见报道,但与其它物种基因编码的蛋白质具有高度的序列同源性。

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