叶原体

Secondly, according to the stem and leaf anatomy, leaf epidermis, pollen morphology and floral development, it is found that there are no close relations between Acorus and Typhales, especially in the aspect of floral development. In the course of floral development of A. calamus, neither bracteloe primordium nor common primordium occurs in the whole course. Early development of the flower is strongly unidirectional. Three shield carpels develop into a pistil. In Sparganium fallax, each female flower has a bracteole. The bracteole primordium and the floral primordium originate from a complex structure. A single saccate carpel develops into a pistil. In staminate flowers, petal and stamen primordia initiate spirally.

菖蒲属与香蒲目的茎叶解剖特征、叶表皮特征、花粉特征及花器官发生特点等亦存在着明显区别，特别是花器官发生特点差异尤为显著，如菖蒲属花器官发生过程中，没有苞片原基，也不存在任何形式的复合原基，花被与雄蕊原基均以单向性方式发生，雌蕊由3心皮发育而成，具有"盾状心皮"的特点等，黑三棱属则有苞片原基发生，且苞片原基与雌花原基起源于共同的复合结构，雄花的花被原基和雄蕊原基均以螺旋状方式发生，雌蕊由单心皮发育而成，未发现有2或3个心皮原基或其痕迹的现象，体现出"囊状心皮"的特点。

The result indicates that spore germination shapes prothallium at keeping wet, cortrolling temperature and covering with 75% shading rate.

结果表明：在保湿、控制温度、覆盖遮光率为75%的遮阳网的条件下孢子发芽率高，能正常形成原叶体，继续培养均能进入有性世代形成孢子体幼苗，其中在山林腐殖土和壤土按1：1的比例混合的基质上最适于孢子萌发和幼苗生长，出苗量可达3500株/m2；在杭州采集的紫萁孢子萌发后形成的原叶体、孢子体均比在湖北民族学院采集的耐高温性好。

The spore germination and gametophyte development of 21 ferns are studied. It indicates that :① the development period of prothallium and young sporophyte of the same species is different due to seeding time of spores;② the optimal temperatures of spores germination and gametophyte development of 21 ferns are about 15 -24 ℃;③ the rate of spore germination of rare ferns is much lower than the ferns which occur large populations in nature;④ the treatment with GA 3 can accelerate the germination of the spores;⑤ the prothallium changes from large to small, green to yellow when young sporophyte comes out. The prothalliums of all 21 ferns die away after the appear of the 3rd leaf of sporophytes;⑥ shapes of the 1st and 2nd leaves of the young sporophyte are different from those emerged later;⑦ spores cultured in dark can not germinate;⑧ a prothallium can develop only one young sporophyte though it has many archegoniums, and the spore propagation with leaf mould substrate is an economical method.

摘 要：以腐叶土为培养基质，对 21 种蕨类植物进行了孢子萌发和原叶体发育的研究，结果表明：①不同时期播种的同种蕨类的孢子，发育出原叶体和幼孢子体所历经的时间长短不同；②孢子萌发和配子体生长发育的适宜温度约为 15 ～ 24 ℃；③稀有蕨类的孢子萌发率低，而在野外能形成较大种群的蕨类的孢子萌发率高；④用 GA 3 处理孢子可以促进萌发；⑤当原叶体上长出幼孢子体时，原叶体由大变小，由绿变黄， 21 种蕨类的原叶体都在幼孢子体上长出第 3 片叶时消失；⑥幼孢子体上长出的第 1 、 2 片叶在形态上与以后长出的叶不同；⑦孢子萌发需要光；⑧ 1 片原叶体尽管有多个颈卵器，但仅发育出 1 株幼孢子体；⑨利用腐叶土进行蕨类孢子繁殖是一种经济实用的繁殖方法。

Based on the tissue culture, the effect of N-phenyl-N＇-1,2,3-thiadiazol-5-ylurea, 6- benzyla minoprine（6-BA） and 1-Naphthylacetic acid on spore germination, protonema develop- ment and bud differentiation in Entodon challenged was investigated in this paper. The whole process was observed and taken pictures. The results show that TDZ, 6-BA and NAA have no significant effect on the spore germination. During the process of protonema development, 1.0 mg/L NAA can promote the primary development of protonema significantly, and 0.4 mg/L TDZ promotes branches formation in inter- mediate stage of the protonema development.

：研究了3种植物生长调节剂苯基噻二唑基脲、6-苄基腺嘌呤（6-BA）、萘乙酸对密叶绢藓孢子萌发、原丝体发育及芽体发生的影响，并对整个发育过程进行了显微观察和照相，结果表明：（1）3种植物生长调节剂对密叶绢藓孢子萌发影响不显著；（2）在原丝体发育阶段，1.0mg／LNAA对原丝体初期的发育促进效果显著，0.4mg／LTDZ对原丝体发育中期分枝的形成促进效果显著，6。

Sinense, the spores germinate inside their exospores and then divide into massive protonemata;the massive protonemata can form two kinds of protonemata of which one is clubbed protonema with papillae,and the other is caulonema composed of long and cylindrical cells ; the gametophyte archaeocytes only occur on the massive protonemata.

结果表明：中华缩叶藓的孢子在壁内萌发，随后分裂产生块状原丝体；块状原丝体上可产生两种丝状体，一种是具疣的棒状原丝体，另一种是由长圆柱状细胞组成的轴丝体；配子体原始细胞只产生于块状原丝体上。

In heterosporous plants, the microspores give rise to small male prothalli bearing male sex organs, and larger female sex prothalli bearing female sex organs.

在具异形孢子的植物中，小孢子形成具小的雄性器官的雄原叶体，而大孢子形成大的具雌性器官的雌性原叶体。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

In this paper the authors report on the biological characteristics of Al-sophila spinulosa Hook.

孢子—原叶体—孢子体的生活周期长，并且在丝状体、原叶体和幼孢子体阶段，无特殊结构去抵抗和忍耐一切不利环境因子的影响。3。

Young embryos can be observed from the prothalli 1 month after sowing.

原叶体受精后1个月内即可观察到从原叶体上生成的幼胚。

The young prothalli are asymmetric and the mature are cordate and naked.

幼原叶体不对称，成熟原叶体裸露，无毛状体，呈心脏形。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。