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Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配体-受体结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受体;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受体为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配体一受体后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受体;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;体外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

In production, larger, self-regulating SCR, or saturable reactor transformers can be used.

在生产中,可使用较大的自调整 SCR 或饱和电抗器变压器。

Wagyu meat is striking because of its wonderful marbling which results in a never-before-experienced succulence that sends the taste buds reeling.

其肉多汁细嫩、其脂肪中多为不饱和油脂,在室温下即可融化,从而让肉质异常滑软,同时也可作为低胆固醇的菜品。

Petroleum resins have certain unsaturation property and can be used as paper glutting agents, plastic modifiers etc.

树脂具有一定的不饱和性,可用于纸张上胶剂,塑料改性剂等。

An unsaturated primary alcohol present in wood spirit; use to make resins and plasticizers and pharmaceuticals.

木醇中一种主要的不饱和醇,用于制树脂、可塑剂和药品。

The regenerated cellulose film prepared by cuprammonia method is firstly undergone plasticizing and drying then coated on two sides with the mixture of degradable low mole-cular weight polyurethane and unsaturated polyester, the water proof film is obtained after solidifying and crosslinking.

按铜氨法制备再生纤维素膜经增塑、干燥后用可降解性的低分子量聚氨酯和不饱和聚酯混合涂料在膜两面涂布,固化交联得到防水膜。

A widely used durable synthetic resin derived from glycerol and phthalic anhydride.

木醇中一种主要的不饱和醇,用于制树脂、可塑剂和药品。

This book discusses the saturable reactor controlled the basic principles and theoretical analysis.

本书论述了可控饱和电抗器的基本原理和理论分析方法。

Hydroesterification of tert-butyl alcohol with ethanol catalyzed by transition metal triphenylphosphine complexes were investigated in room temperature ionic liquids as reaction medium at 100-140℃ and 3-6MPa of CO.

首次在超强酸氯铝酸离子液体中实现了饱和烷烃的羰化反应。发现2,2,4-三甲基戊烷可直接与CO反应,产物叔丁基异丁基酮,叔丁基异丁烯基酮和3,3,5-三甲基环己酮。

A single-longitudinal mode fiber laser with fiber saturable absorber is designed. Tunable fiber Bragg grating as the wavelength selector is in the ring cavity with a 2 m-long unpumped erbium-doped fiber.

设计了一种基于光纤饱和吸收效应的环形单纵模光纤激光器结构,以可调谐光纤布拉格光栅作为波长选择器件,与2 m未抽运掺铒光纤一起位于环形腔反射臂上。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。