可酸化的

1,6-Dimethyl adipate with selectivity of 100% was synthesized by batch and continuous esterification process using adipic acid and methanol as raw materials,DNW-I strong-acid cation exchange resin as catalyst.

摘 要：以DNW-I型强酸型正离子交换树脂为催化剂，己二酸和甲醇为原料，经间歇酯化和连续酯化反应后通过精馏可制备选择性为100%的1，6-己二酸二甲酯。

During the reaction of alkylation, general contained 0.2%~2.0% butadiene will produce viscous heavy composite materials, which will raise final boiling point of alkylate and decrease octane, what is more, it will react with acid to produce corresponding ester, which will increase the consumption of acid, Therefore, we must remove butadiene by selective hydrogenation, at the same time, it will convert some of 1- butane to 2- butane by isomerization and raise octane of alkylate.

一般含有0.2%~2.0%的丁二烯，其在烷基化反应中，可生成一种分子量很高的粘稠重质迭合物，使烷基化油的干点升高，辛烷值降低，并且它还和酸反应生成相应的酯而加大酸耗量。因此必须经过选择性加氢除掉丁二烯，同时，将部分1-丁烯异构化为2-丁烯，以提高烷基化油的辛烷值。

After esterification, the ethyl-2-chloro-3-phenylpropionate reacts with diethyl malonate under the catalysis of sodium ethylate, and we obtain the corresponding triethyl ester. The-benzylsuccinic acid is formed by the triethyl ester reacting with concentrate hydrochloric acid and taking place hydrolyzation and decarboxylation reaction at the same time. In order to avoid the formation of cinnamic acid, the sodium ethylate and diethyl malonate must be react sufficiently. The course has simple operation, inexpensive materials and better yield.

具体制备工艺为：首先以L-苯丙氨酸为原料采用重氮化方法得到-2-氯-3-苯基丙酸，再经酯化得到-2-氯-3-苯基丙酸乙酯后在乙醇钠催化作用下和丙二酸二乙酯缩合，发生SN2反应，得到相应的三乙酯，然后经水解和脱羧，得到-苄基丁二酸；在实验中乙醇钠要和丙二酸二乙酯充分反应，避免生成肉桂酸乙酯；该过程操作简单，原料便宜，产率较高，总收率可达到26.8%。

PartⅡThe mechanism elucidation about effects ofα-(2,3)/(2,6)sialic acid on the Cx43gap-junction functions.(1) Westernblotting experiment showed that the decrease of sialic acid didn\'t changethe Cx43 expression and its phospholation level.(2) Westernblotting experiment showed sialidase didn,t change the ZO-1 expression,IP and confocal experiment showed sialidase improved the interaction of Cx43 andZO-1.(3) Westernblotting experiment showed sialidase didn\'t change N-cadherin expression,IP and confocal experiment showed sialidase promoted the complex formation ofCx43 and N-Cadherin.(4)Sialidase could increase the ERK1/2 phospholation level,and enchanedintercellular homotypic adhesion,Immunofluorometric assay showed sialidase couldpromote the N-cadherin cluster on the membrance.

第二部分：α—(2，3)／(2，6)唾液酸对肿瘤细胞CX43间隙连接功能影响的机制研究1、Westernblotting结果表明降低细胞膜表面唾液酸并不改变Cx43的表达及其磷酸化水平：但Cx43连接斑形成增多；2、Westernblotting结果表明唾液酸酶作用后细胞ZO—1表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与ZO-1的结合；3、Westernblotting结果表明唾液酸酶作用后细胞N-cadherin表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与N-Cadherin复合物的形成；4、唾液酸酶作用后细胞内ERK1／2磷酸化水平明显增加，细胞间同质粘附增加，以及免疫荧光表明唾液酸降低后可促进N-cadherin的膜成簇。

Results The synthesis condition was improved. The method of methane sulfonic acid/DL-methionine was found an ideal substitute of BBr3 for less cost. While using hydrobromic acid, the O-demethylation and hydrolyzation could be combined in one stage.

结果 优化了纳洛酮的合成工艺，甲磺酸／DL-甲硫氨酸法试剂价格低廉，可降低生产成本；而氢溴酸法不但试剂价格更低，还可将去氧甲基化和水解合并为一步反应，使新工艺的总收率提高到59.90%，产品质量符合中国药典。

Prophenoloxidase is a key enzyme for mediating melanotic encapsulation of oocysts,which may be transformed into active phenoloxidase by limiting proteolysis.

而前酚氧化酶(Prophenoloxidase，PPO)是介导卵囊黑化包被反应的一种关键酶，活化后可催化酪氨酸羟基化为二羟基苯基丙氨酸，二羟基苯基丙氨酸和多巴胺可进一步氧化为具有活性的苯醌活性，苯醌即可介导蛋白交联并聚合成黑色素层固定、隔离或杀死入侵的病原体。

Niobic acid as a solid catalyst can be directly applied in the hydrolysis, esterification, condensation and alkylation reaction etc.

铌酸可直接作为固体催化剂，应用于水解、酯化、缩合、烷基化等反应中；铌酸也作为载体负载金属，应用于F-T合成、氧化反应和加氢等反应中；含铌化合物也可作为负载型催化剂，负载到不同类型的载体上；含铌化合物也作为助剂或光催化剂，广泛地应用于催化多种化学反应。

The results show that the SAPO–5 has a pillar-like morphology with the size of about 20 μm×5 μm×5 μm.The ratio of P:Al:Si is 1:1.19:0.67 in its crystal structure, the content of Si is high. The total acidimety fo the SAPO–5 is 578.0 μmol/g , hihger than that of prepared by one step crystalline hydrothermal method.And its initial catalysis character performance on normal butane isomerization reaction reach 24.8 %, higher than that of prepared by one step crystalline hydrothermal method.

实验结果表明：产物为柱状的磷酸硅铝分子筛，其晶粒尺寸大约为20 μm×5 μm×5 μm，其晶体结构中P：Al：Si＝1：1.19：0.67，硅含量较高；采用分段晶化方式制备的SAPO-5分子筛的总酸量为578.0 μmol/g高于一段晶化方式制备的SAPO-5分子筛的总酸量；采用分段晶化生成的SAPO-5分子筛的催化正丁烷异构化反应初始活性可达到24.8 %，远高于一段晶化制备的SAPO-5分子筛的初始活性14.1 %。

1、Cur inhibits K562 cells growth and induces cell apoptosis may be correlated with the down-regulation of p210~、inhibition of protein tyrosine phosphorylation and the signaling molecules such as p-Erk1/2、c-myc which are relevant with cell growth and apoptosis; 2、Cur synergizes STI571 to inhibit K562 cell growth and induce cell apoptosis may be correlated with the down-regulation of p210~、inhibition of protein tyrosine phosphorylation and the signaling molecules such as Hsp90、PKC which are relevant with cell growth and apoptosis; 3、Cur reverses the resistance of K562/G01 cells to STI571, and synergizes STI571 to inhibit K562/G01 cell growth and induce cell apoptosis; 4、Cur inhibits human originated CML CD34~+ cell growth、induces cell apoptosis, and enhances STI571 to down-regulate the expression of p210~, finally inhibit cell growth and induce cell apoptosis.

从以上实验结果我们得出如下结论： 1、Cur抑制K562细胞增殖、诱导细胞凋亡的作用可能与其下调p210~、蛋白酪氨酸磷酸化水平以及抑制下游p-Erk1/2、c-myc等信号分子有关； 2、Cur协同STI571抑制K562细胞增殖、诱导细胞凋亡的作用可能与其下调p210~、蛋白酪氨酸磷酸化水平以及抑制Hsp90和下游PKC等信号分子有关； 3、Cur可逆转K562/G01细胞对STI571的耐药性，并与STI571协同抑制K562/G01细胞增殖和诱导凋亡，其抑制K562/G01细胞增殖、诱导细胞凋亡的作用可能与其下调p210~、蛋白酪氨酸磷酸化水平以及抑制下游Procaspase-3和NF-κB等信号分子有关； 4、Cur可抑制来源于CML患者骨髓的CD34~+细胞的增殖并诱导其凋亡，还可协同STI571下调CML CD34~+细胞p210~表达，进而协同抑制细胞增殖、诱导细胞凋亡。

Novozym 435 was used for the synthesis of vitamin A plamitate. The influences of solvent, the molar ratio of substrates, the reaction temperature and time, and the water concentration were optimized and the best result was obtained by interesterification from 0.200 g vitamin A acetate and 0.468 g palmitic acid, at 30℃, in 10 mL hexane, containing 0% of water, with 10% of lipase(mass ratio, immobilized lipase to vitamin A plamitate). In these conditions, 75% of vitamin A acetate was converted into vitamin A plamitate. The immobilized lipase was reused about 6 batches.

对催化合成维生素A棕榈酸酯反应的脂肪酶和反应介质比较，同时对影响合成维生素A棕榈酸酯反应的因素（温度、初始水含量、底物摩尔比、反应时间和酶量等）进行了探讨，优化了反应条件：在10 mL不含水分的正己烷中，0.200 g 维生素A醋酸酯和0.468 g棕榈酸在酶量为10％（指固定化酶与维生素A醋酸酯的质量比）的固定化脂肪酶催化下，在 30℃、190 r/min下反应6 h，转化率可以达到75%，固定化酶可连续使用6次以上。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。