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During the postgenomics era the information about genomic sequence and gene functions provides a new foundation for evolutionary biology and ecology As the first whole-genome sequenced plant Arabidopsis thaliana and its wild relatives have played a critical role in understanding the evolution of genomics and speciation Both A halleri and A lyrata are closely related to the model species A thaliana A halleri ssp gemmifera occurs in northeastern China Japan and Taiwan; while its sister A halleri ssp halleri is mainly distributed in Europe Geographical barriers such as Tienshan Mountain Range isolate these intraspecific sisters Likewise A lyrata ssp kamchatica and ssp lyrata occur in East Asia and North America respectively Such distribution patterns seem to be consistent with allopartic speciation The comparison between ancestral and extant polymorphism by multilocus can be informative about the population genetics of speciation In this study we collected and analyzed DNA sequences of 98 genes from four wild relatives of A thaliana A halleri ssp gemmifera A halleri ssp halleri A lyrata ssp kamchatica and A lyrata ssp lyrata The ancestral states of these four species were compared to each other in terms of level of genetic variation However the ancestral species at the time of speciation were substantially more polymorphic than the extant geographical populations The observations are not fully compatible with speciation by strict allopatry At some species pairs parapatric speciation seems more reasonable in speciation of Arabidopsis The 98 gene sequences are also used for the congruence test between gene genealogy and species phylogeny Only 28 genes support the species phylogeny but there are 23 genes supports another major genealogy { lyrata} thaliana Based on the phylogenetic position change of A lyrata ssp kamchatica and Ks value for each species pair suggested the recent directional gene flow between A halleri ssp gemmifera and A lyrata ssp kamchatica

阿拉伯芥是第一个完成基因体定序的开花植物,其基因体资讯提供植物学研究的重要依据;在解析阿拉伯芥属物种的亲缘关系以及种化机制等重要的演化议题时,阿拉伯芥近缘的野生物种自然成了不可或缺的关键;跟阿拉伯芥近缘的物种包括A halleri及A lyrata,其中A halleri ssp gemmifera主要分布於中国东北、日本以及台湾,与近缘的A halleri ssp halleri其分布於欧洲隔著天山及大陆的障蔽,而A lyrata ssp kamchatica主要分布於东北亚及台湾,与分布於北美五大湖的A lyrata ssp lyrata被北极圈所分隔,这样的分布模式暗示异域种化的可能。藉由多基因分析比较祖先物种与现生物种遗传歧异度的相关可提供讯息探讨种化时期的族群遗传结构,本研究针对A halleri ssp gemmifera、A halleri ssp halleri及A lyrata ssp kamchatica、A lyrata ssp lyrata四个物种,两对互为亚种的姊妹群,以阿拉伯芥为外群进行研究,在四个物种完成98个同源基因的分子序列,利用套装软体MCMCcoal来估算祖先物种的遗传变异,亦估算现生物种的核苷酸歧异度,观察到?多物种配对中祖先物种遗传多型性大於现生物种DNA歧异度,显示异域种化模型并无法完全解释阿拉伯芥属物种的种化模式,在某些物种配对间邻域种化模式应比异域种化更为可能;在基因树与物种树的比较,98个基因片段的亲缘模式只有28个是与已知物种树一致的,有23个基因其树状图支持{ lyrata} thaliana的型式,藉由kamchatica位置的变化以及估算各物种配对间的平均同义置换率,推测在A halleri ssp gemmifera与A lyrata ssp kamchatica间具有近代的单方向基因交流。

The RAPD was used to study the genetic diversity of 19 KinXin series potato cultivars. The result showed that 52 RAPD bands were amplified using 7 primer, among them 43 bands were polymorphic, and 4~9 bands were amplified in each lane. The RAPD analysis indicated that the genetic distance of 19 potato cultivars was between 0.17~0.72, average value was 0.39. When all of the polymorphic bands were analysed by cluster analysis, 19 potato cultivars were classified into 3 groups. The analysis showed that there existed close sibship among the majority of potato cultivars, and the genetic distance between the minority cultivars and the others is longer, which indicate that the genetic basis of KeXin series potato cultivars is wide.

利用RAPD标记技术对19份克新系列马铃薯品种进行了遗传多样性分析,分别提取19份马铃薯品种的DNA,进行随机引物多态性扩增,从1000条随机引物中初步筛选出7条有多态性的引物进行详细研究,每条RAPD引物扩增出4~9条带,共获得52条带,其中多态性条带为43条;19份马铃薯品种的遗传距离介于0.17~0.72之间,平均值为0.39,平均遗传距离介于0.31~0.51之间;聚类分析结果在GS=0.53处可将克新系列马铃薯品种划分为三类,聚类结果与系谱分析基本相符,同时也说明克新系列马铃薯的遗传基础有所拓宽。

Finally the new line of fruit fly with 3 recessive characters (white-eye, winglet and ebony-body) was purified.

以得到的白眼、小翅、黑檀体三隐性新品系为材料,可同时满足三大遗传规律(分离规律、自由组合规律、连锁遗传规律)和性连锁遗传等实验教学的需要。

Genetic cluster analysis is similar based on the results of morphological and DNA markers, the five populations could be divided into three groups. But there are different groups sort of individual locations, reflecting the similarities and difference about the analysis of the relationship between population with the two kinds of markers, It could be concluded that there was coupling and relevance to some extent in evaluating genetic diversity with the same experiment sampling by morphological and SSR marker variation. However, there was also differences. In research, the two can complement each other.8. Identification of Genetic Relationship of Amygdalus plants by SSRThe genetic relationship among 55 Amygdalus plants collections, belonging to 6 species and collected from both homeland and abroad were identified via SSR molecular marker technique.

新疆野扁桃遗传多样性不同研究分析方法结果的比较与评价表型与DNA二种水平的遗传标记方法揭示的遗传多样性水平有差异,表型性状比DNA分子标记具有更大的变异性;其受到环境的影响较大,也即对环境更敏感。2种水平的遗传标记聚类结果将野扁桃5个不同分布群体均可分为3个大类群,但是出现有个别不同的群体排序位置,反映了2种标记在分析种群间关系上的异同性,从本研究结果来看,说明表型与DNA标记有一定的耦合性和相关性,但也存在差异,在研究上可以互为补充。8。

The colony morphology, growth rate and homothallic characters were all steadily inherited in both single-zoospore and single-oospore progenies,which indicated that the genetic factors of controlling the three characters were homozygous.

结果表明,菌落形态、生长速率和同宗配合性状在单孢后代可稳定遗传,控制上述性状的遗传因子是纯合的;大豆疫霉菌的游动孢子产生能力在单孢后代发生连续性变异,表明这种性状可能是数量遗传性状,也可能控制这种性状的基因为杂合基因或细胞质遗传因子。

There was great diversity index among genetic trait of 50 barley. The anomic trait used as tested materials was analyzed by cluster. Cluster analysis showed that 50 barley could be classified into 5 groups at the level of GD 3.28; The 50 barleys were analyzed by A-PAGE to study genetic diversity of hordein.

各农艺性状的遗传多样性指数都较大,具有丰富的遗传多样性;对供试材料的农艺性状进行聚类分析,50份大麦材料在遗传距离3.28时可被聚为5大类。

Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出,等位基因平均值、观察杂合度、Fis值、Fit值皆较高,表明非洲菊等位基因较丰富,杂合基因偏多,且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium;PP8、na、Ⅰ、h、Gi及Fst皆表明,ISSR检测遗传多样性的能力最强,其次是RAPD,等位酶最低;根据遗传距离进行聚类,三种方法都把70个品种分成A、B、C、D、E五个大组,每一组中除少数品种发生位移外,大部份品种分类结果相似,且与形态分析结果有相似性,但在每一组中,品种间的聚类差别较大,表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同;Mantel检测表明,三种标记的分析结果有显著相关性,表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异;非洲菊为组培苗,三种标记的检测结果皆表明,混合样品可以作为个体样品的代表,对整个居群的遗传多样性进行评价;这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

The genetic analysis and improvement of characters, such as tuber processing quality traits and yield components which tend to be quantitatively inherited, are far more difficult than those at diploid potato. Reviewing the earlier studies indicated that a few systematic investigations on genetics of important traits in potato were untaken and most focused on estimates of combing ability for agronomic traits due to limited materials and methodologies available.

利用二倍体材料简化了遗传分离比例、可应用现有的二倍体遗传模型进行遗传分析并更有效的挖掘和利用二倍体资源中的优异基因,进而通过倍性育种及遗传工程育种将有利性状转育到四倍体普通栽培品种。

The 25 acces-sions were classified into three major groups: A, B, and C by cluster analysis using UPGMA, which showed significant relationship with the origin regions of accessions. Thus, it was suggested that the Sichuan hulless barleys could be used as elite germplasms to enhance the genetic background for super-hulless barley breeding.

聚类分析结果表明, 25份材料可分成A、B、C 3大类,材料聚类与其来源地有明显的相关性。25份材料间的平均遗传距离较小(0.3240),平均遗传多样性较低(0.5126),遗传基础较为狭窄。

Among the four characteristic bands,air-cured tobacco including maryland tobacco and Burley tobacco,sun cured tobacco including oriental tobacco and sun-cured tobacco,and flue-cured tobacco and air-cured tobacco were distinguished.

两条晾烟类型的特征标记带、一条晒烟类型的特征标记带,一条烤烟与晾烟特征标记带。这些标记不但可以直接转化成SCAR标记,而且可通过分离群体的遗传分析用于烟草类型间遗传转化的特征带,也可进一步用于测序发掘新基因。

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