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The study suggest:(1) The observation of satellites in root tip and multiplex PCR makers could accurately identify the homozygous and the heterozygosis of 1BL/1RS translocation.(2) Through the restoring degree we could confirm the success of the transfer. The combination of the restoring degree and the agronomy property could increase the efficiency of practical application.(3) This study proposed the transformation from the physiological male sterile to the genetic male sterile, and then establish a method of utilize the strong superiority crosses.

其中复合引物PCR更适合于回交后代中大量目标种子的筛选;(2)恢复基因转育后代定株与不育系测交,依据测交恢复度可准确确定恢复基因在回交后代的延续,恢复基因定株定向转育与性状选择结合可大大提高转育后代的实际应用效果;(3)本研究提出的粘类小麦雄性不育恢复基因Rfv1定向转育体系,可有效地实现小麦由化控强优势组合向三系强优势组合的定向转化,即生理型不育途径向遗传型不育途径的定向转化,进而建拓一套杂交小麦强优势组合多途径利用新体系。

To disease resistance, SP3 (from H1) had stronger ability to resist leaf mold. 4.The characters obtained by space mutation were easier to be stable. The results showed that, the differences of the flowering dates of 1st inflorescence, the positions of 1st inflorescence, number of leaves between 1st and 2nd inflorescences in the colony were decreased with the development of generations. Most of these heritable characters could be stable in SP3 and a few could be stable in SP4, 2~3 generations earlier than that of traditional breeding (The characters were stable in 6 generations.).

试验结果表明,植株的第一花序开花期、植株的第一花序节位、第一至第二花序间隔叶数及产量的株间变异(变幅、标准差和变异系数),随着世代的递增而降低,大部分株系的变异性状从SP3后就稳定遗传,少数株系的变异性状从SP4后才稳定遗传,比一般常规育种的6个世代开始稳定,可提早2~3个世代,经地面定向选择能较快选出综合性状优良的目标株系。

Producing PHAs,and then were orientationally inserted into a prokaryotic expression vector pBV220. By analyzing the bioactibity and function of their products, the biological function of phaA, phaB and phaC was confirmed. These have been done in our laboratory. To make better use of the three genes by plant bioreactor, two groups of different plant expression vectors that can be used to produce PHAs in different plants were constructed, and the transformation of PHA in Oxalis triangnlaris has been preliminarily reseached.The following is the main results:Three plant expression vectors pBI121-A,pBI121-B and ubquiting-C, which can be used to be transformed into Oxalis triangularis were respectively constructed by using the phaA, phaB and phaC.

菌株的亚克隆基因组片段中,分离出phaA、phaB和phaC三个基因片段,定向克隆至原核表达载体pBV220,通过对其生物活性与功能分析,确证了基因phaA、phaB和phaC的生物学功能;为了通过植物生物反应器对这三个基因进一步研究利用,本实验利用这三个基因构建了两组可用于不同植物生产PHA的真核表达载体,并对紫叶酢浆草进行PHA基因遗传转化的初步研究,获得主要结果如下:利用克隆的phaA、phaB、phaC三个基因分别构建了可用于转化紫叶酢浆草表达载体pBI121-A、pBI121-B、uBquiting-C;可用于玉米转化的种子特异性表达载体p7S-A、pBI121-S-B、uBquiting-C。

The fact that the pink plants were predominant among the population of RP1 cross progeny hints that the pink had the strong descendiblity, Whereas another fact that the plants with the color of another parent were predominant among the population of R01 cross progeny indicates that the nacarat/saffron plant had the low descendiblity. The above results suggest that it is better to choose the pink plants with the target color flower of breeding as a parents when arranging the cross combination. Many novel flowers with singular value of view and admire can be bred by the different cross combination since the wide segregation chromatogram is not confined to the parents'.

以RPl及R01作母本或父本,分别与不同花色杂交,F1代发生了明显的花色分离,RP1的杂交后代均以粉色占优势,表明粉色的遗传力较强,R01的杂交后代均以其它亲本占优势,表明桔黄/桔红的遗传力较弱,因此在选配组合时,最好选择具有育种目标的粉色个体作为亲本;另外由于分离谱较广,且分离的色谱范围并不完全限于双亲之间,可根据不同的杂交配组,培育出具极高观赏价值的稀有花色。

The splicing/decomposible encoding scheme proposed by Xu et al is applied to the construction of dynamic selection and mutation operators that were built respectively on the levels of genotype and phonotype of population.

简要介绍了徐宗本教授新近提出的可分解/可拼接遗传算法编码,并证明了一个利用对偶适应函数判定已知个体是否为相对全局最优解的判据。

At least half of patients with X-ALD are adults with somewhat milder manifestations, and women who are carriers may become symptomatic. X-ALD is often misdiagnosed as attention-deficit/hyperactivity disorder in boys and as multiple sclerosis in men and women, and is not an uncommon cause of Addison disease;(2) the incidence of X-ALD, estimated to be 1:17 000 in all ethnic groups, approximates that of phenylketonuria;(3) noninvasive and presymptomatic diagnosis and prenatal diagnosis are available; family screening and genetic counseling are key to disease prevention; and (4) new therapies, applied early, show promise.

至少1半的X-ALD为成年病人,表现较轻,而作为携带者的女性也可发病。X-ALD在男孩常误诊为注意缺陷/多动综合征,男女可误诊为多发性硬化,并且也是Addison病的病因;(2)据估计X-ALD发病率为1:17 000,类似于苯丙酮尿症;(3)可进行无创性、症状前诊断和产前诊断;家系筛查和遗传咨询是疾病预防的关键;(4)早期采用新的治疗方法非常具有前景。

At least half of patients with X-ALD are adults with somewhat milder manifestations, and women who are carriers may become symptomatic. X-ALD is often misdiagnosed as attention-deficit/hyperactiity disorder in boys and as multiple sclerosis in men and women, and is not an uncommon cause of Addison disease;(2) the incidence of X-ALD, estimated to be 1:17 000 in all ethnic groups, approximates that of phenylketonuria;(3) noninasie and presymptomatic diagnosis and prenatal diagnosis are aailable; family screening and genetic counseling are key to disease preention; and (4) new therapies, applied early, show promise.

至少1半的X-ALD为成年病人,表现较轻,而作为携带者的女性也可发病。X-ALD在男孩常误诊为注意缺陷/多动综合征,男女可误诊为多发性硬化,并且也是Addison病的病因;(2)据估计X-ALD发病率为1:17 000,类似于苯丙酮尿症;(3)可进行无创性、症状前诊断和产前诊断;家系筛查和遗传咨询是疾病预防的关键;(4)早期采用新的治疗方法非常具有前景。

Phylogenetic analysis revealed that cytb gene was helpful to distinguish validly between S.japonicum isolated from mountainous areas and lake areas in mainland China.The cytb gene could also distinguish S.japonicum from other trematodes belonging to the family Schistosomatidae.

种系发育关系分析发现,依据cytb基因可有效地区分我国日本血吸虫山区和湖区两种流行类型,显示种内遗传变异特点,并可将日本血吸虫与分体科的其他吸虫鉴别开来。

The ISSR fingerprinting of 18 Japanese plum cultivars and 12 plum species or variety were established by using optimum system with primer 5"-9T-3"and 5"-9C-3", respectively. The identification rate of plum cultivars and species or variety was 100%. The effects of five important components on reaction of SSR were studied with Prunus salicina cv. Meilili. The results showed that the optimum concentration of five important components i.e. Tag DNA polymerase, Mg +, single primer, template DNA and dNTPs in 25 μL reaction system of SSR were 1.5u, 2.0mM, 0.8 μ M, 30~40ng and 0.16~0.24mM, respectively. The dendrograms of these cultivars was contructed according the Jaccard coefficient of similarity.

利用该优化体系,以5′-_9T-3′为引物,构建了中国李18个品种的ISSR指纹图谱,该引物可将这些品种完全区别开来;以5′-_9C-3′为引物,构建了李属6类种质资源的ISSR指纹图谱,该引物区分率为100%。4、中国李品种适宜的SSR反应分析体系是:25μL SSR反应体系中,Taq DNA聚合酶、Mg~(2+)、每个引物、模板DNA和dNTPs等5种成分的适宜浓度分别是:1.5u、2.0mmol·L~(-1)、0.8μmol·L~(-1)、30~40ng和0.16~0.24mmol·L~(-1)。5、采用RAPD、ISSR和SSR等三种分子标记技术对中国李品种资源54份和2个欧洲李品种进行了遗传多样性分析,并以品种间的遗传相似系数构建了Jaccard聚类树状图。

An acceptable DNA fingerprint was constructed with 2 primers that could be used to identify these Au-ricularia auricula cultivars, all cultivars were distinguished from each other and showed genetic diversity.

用NTSYS软件进行聚类分析,可将14个供试菌株分为2个类群,类群Ⅰ包括11个菌株,且其可进一步划分为3个亚类;类群Ⅱ包括3个菌株;其中&981&和丰收1号的遗传距离最近。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?