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The typical apoptotic morphological features appeared in MUTZ1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dosedependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dosedependent manner, the cells were arrested at G0/G1 phase.

结果显示: VPA对MUTZ1细胞的生长抑制作用呈现时间和剂量依赖性;经4 mmol/L VPA处理MUTZ1细胞72小时后,细胞呈现典型的凋亡形态特征,光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体;透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加,胞浆内大小不规则的染色质团块;流式细胞术结果表明,细胞凋亡率随着VPA浓度的增加而逐步增高,G0/G1期细胞比例随着VPA浓度的增加而逐渐增多,S期细胞比例逐渐减低,细胞被阻滞在G0/G1期。

Results: IL-8 protein was located in the epithelium of viii. The expression of IL-8 was significantly increased in RSA group than that in the control group. Positive IL-8 cells was shown in decidua of RSA group, and its IL-8 level was higher than that in the control group. By hematoxylin-eosin staining. trophoblastic layer was found to get thinner, cells of trophoblastic layer denatured or necrotized, and turned acidophily, and the fibration of villous axis increased in RSA; decidual cells lost connection, a part of decidual cells appeared cytoclasis and turned more acidophilic, and nuclei disappeared in RSA.

结果:在绒毛组织中,IL-8蛋白定位于绒毛上皮细胞的细胞质内,且病例组的表达明显高于对照组;在蜕膜组织中;病例组蜕膜细胞的胞质内可见IL-8蛋白表达,且高于对照组;H-E染色可见病例组绒毛组织的滋养层变薄,细胞变性甚至坏死、嗜酸性增强,绒毛中轴纤维化程度增强;蜕膜组织中蜕膜细胞失去细胞间连接,部分蜕膜细胞解体、核消失。

Immunocytochemical staining was used to identify sarcomeric actin and intercalated disc-like structure when the cells were cultured for additional 1 week, 2 or 3 weeks, respectively. RESULTS: Sarcomeric actin positive cells were observed in 1 week, 2 or 3 weeks after 5-aza treatment. Some cells stained positive for connexin43 at 1 week and 2 weeks after 5-aza treatment, with brown pellet located dispersively around nucleus.

结果: 5-aza处理后1周、2周、3周均可见横纹肌肌动蛋白染色阳性细胞。5-aza处理后1周及2周连结蛋白43在部分细胞上表达:呈核周散在分布的棕黄色颗粒,5-aza处理后3周连结蛋白43 在细胞密度大的区域呈核周聚集成线形分布的棕黄色颗粒,个别细胞间可见此结构,类似正常心肌的闰盘结构。

The results showed the radioscaphocapitate,radiolunate,ulnolunate,ulnotriquetral and dorsal radioulnotriquetral ligaments were composed of densely fasciculated collagen fibers which contained more elastic fiber.

结果显示,桡舟头韧带、桡月韧带、尺月韧带、尺三角韧带和腕背侧桡尺三角韧带中均可见致密粗大的胶原纤维束,束内含有大量的弹力纤维;桡舟月韧带中缺乏弹力纤维,但含有丰富的血管;腕尺侧副韧带中无明显的胶原纤维和弹力纤维可见。

Affect on the liver pathological state after XIEZHIPING treated: the golden hamster hepatic tissue morphous structure of normal control was normal, which had regular hepatic lobules and no lipid droplet;that of model control manifested diffuse heavy adipose degeneration and heavy ballooning degeneration with inflammatory cell infiltrate which mainly composed of lympholeukocyte;that of high dose group showed midrange ballooning degeneration with chronic inflammatory cell infiltrate which mainly composed of lympholeukocyte and plasmocyte;that of media dose group indicated slight focus ballooning degeneration with a small quantity normal hepatic cells in some locals;that of low dose group appeared midrange adipose degeneration with a few lympholeukocyte cell infiltrate in the parts of central veins and port area.

泻脂平治疗后对肝脏病理状况的影响:空白对照组金黄地鼠的肝组织形态结构正常,肝小叶规则,无脂滴发现;模型对照组肝脏弥漫性重度脂肪变性,发生重度气球样变,伴见以淋巴细胞为主的慢性炎性细胞浸润;泻脂平高剂量组肝细胞呈现中度气球样变,伴见以淋巴细胞、浆细胞为主的慢性炎性细胞浸润;中剂量组肝细胞轻度灶性气球样变,局部可见少量正常肝细胞;低剂量组肝细胞呈中度脂肪变性,可见中央静脉及汇管区些许淋巴细胞浸润。

Affect on the liver pathological state afterXIEZHIPING treated: the golden hamster hepatic tissue morphous structureof normal control was normal, which had regular hepatic lobules and nolipid droplet: that of model control manifested diffuse heavy adiposedegeneration and heavy ballooning degeneration with inflammatory cell infiltrate which mainly composed of lympholeukocyte; that of high dosegroup showed midrange ballooning degeneration with chronic inflammatorycell infiltrate which mainly composed of lympholeukocyte and plasmocyte;that of media dose group indicated slight focus ballooning degenerationwith a small quantity normal hepatic cells in some locals; that of lowdose group appeared midrange adipose degeneration with a fewlympholeukocyte cell infiltrate in the parts of central veins and portarea.

泻脂平治疗后对肝脏病理状况的影响:空白对照组金黄地鼠的肝组织形态结构正常,肝小叶规则,无脂滴发现;模型对照组肝脏弥漫性重度脂肪变性,发生重度气球样变,伴见以淋巴细胞为主的慢性炎性细胞浸润;泻脂平高剂量组肝细胞呈现中度气球样变,伴见以淋巴细胞、浆细胞为主的慢性炎性细胞浸润;中剂量组肝细胞轻度灶性气球样变,局部可见少量正常肝细胞;低剂量组肝细胞呈中度脂肪变性,可见中央静脉及汇管区些许淋巴细胞浸润。

Surface-based hippocampal morphologic analysesWe found regional atrophy of the lateral and inferior hippocampus area bilaterally corresponding to the CA1 and subiculum hippocampal subfield and reginal expand in the heads and tails bilaterally in aMCI compared with NC.

基于海马表面的形态分析结果aMCI组双侧海马的外侧及下表面可见区域性萎缩,这些区域相当于组织学上的CA1区和下托,此外在双侧海马的头部及尾部可见局部膨胀。

In malignant room skin tumour early phase can see something without the aid of instruments in regular or opaque dirty tier or wall have a lot of white or the gray pellet and form festival or the lamina on tier of pleurae Piece with the development of tumour change thickly long and change thickly long full pleura surface tubercle the tumour tubercle stretches room skin to all sides postpone continuing becoming movie cyst lungs uses the person The cellar is getting smaller as shrinking more and makes to be put into much trouble inclining a chest wall subside.

在恶性间皮瘤早期,肉眼可见在正常或不透明的脏层或壁层胸膜上有众多白色或灰色颗粒和结节或薄板块,随着肿瘤的发展,胸膜表面愈来愈变厚,长满结节,间皮瘤结节向四方伸延,连续成片,包囊肺脏使其窖越缩。。。在恶性间皮瘤早期,肉眼可见在正常或不透明的脏层或壁层胸膜上有众多白色或灰色颗粒和结节或薄板块,随着肿瘤的发展,胸膜表面愈来愈变厚,长满结节,间皮瘤结节向四方伸延,连续成片,包囊肺脏使其窖越缩越小

FCM appeared obvious inferior diploid peak and blocked the cells to the G_1 phase.The red particles throughout the cytoplasm and permeated the plasma membrane could also be seen.Chromatin lining and breeding body could be seen under the transmission electron microscope.

FCM出现明显的亚二倍体峰并将细胞阻滞于G1期,Annexin-V-FITC联合法染色可见细胞凋亡的特征性贯穿胞质穿过胞膜的红色颗粒,电镜下可见染色质边集及生发小体的凋亡形态特征。

The results showed that, after induced for 4~5 d by dexamethasone, beta-glycerophosphate and ascorbate-2 phosphate, the volume of some cells of the goat MSCs gradually largened and changed their form from spindle/polygon to cuboid/ovoid; With the induce time continuing, the numbers of the cuboid/ovoid cells continuous increased; At 15th day, there were many cuboid cells, and the modality of the cells changed from cuboid to ovoid; At 25th day, the oval and cuboid cells accumulation were observed.

结果表明,山羊MSCs在地塞米松、β-磷酸甘油和抗坏血酸的作用下,从诱导的第4~5天开始,即可见有些MSCs体积逐渐增大,由梭形或多角形转变为立方形或卵圆形;随着诱导时间延长,立方形或卵圆形细胞不断增多,到第15天时,有较多的立方形细胞,且这种变化经历了从立方形向卵圆形转变的过程;第25天,可见卵圆形和方形细胞堆积,诱导分化率为90.2%± 2.97%。

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And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时,论述了一体化管理体系构建中的几个主要问题,包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。

If you're huffing and puffing at any point during the run, slow down and make sure you can take deep breathes.

如果你任何时候在跑步过程中气喘如牛,降低速度并且确保你能够深呼吸。