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Results After six passages in BHK-21 cells, cytopathic effect was observed by microscopy. The rescued virus was rejuvenated once in unweaned mice, and then came back to cell passage. We found that the poly tract of rescued virus was shortened when the virus was passaged twice in BHK-21 cells. Although the data of LD50 in mice and TCID50 in BHK-21 cells showed that the virulence and infectivity of genetic engineering viruses were lower than its parental virus, no significant difference was observed between the genetic engineering viruses with the different length poly tract.

我们发现,基因工程毒在BHK-21上连续传代至第6代时,可见明显的致细胞病变效应(cytopathic effect,CPE);在经过一代乳鼠接种之后再重新适应到BHK-21细胞的过程中,poly区段出现了缩短现象;乳鼠致病性试验和BHK-21细胞TCID50测定结果表明,尽管基因工程毒与母本毒相比毒力偏低,但含有不同长度poly的基因工程毒之间并无显著差异。

objective in order to investigate the variety of the airborne fungi in jingzhou area and study the relationships between airborne fungi and allergic diseases.methods we surveyed the fungi sensitizing of air in jingzhou city from feb.1th,2007 to jau.31th,2008 to quantitatively observe the daily airborne fungi and count under microscope through one year.results the fungi spores were present through all year.more than 20 taxa of airborne fungi have been collected,among which the most common fungi were uredinales,ustilaginales,alternaria,etc.fungi spores were ralated with the season and weather,the most aboudant period in sring and summer.conclusion the airborne fungi were seen one year and were mostly allergenic.many of then had seasonal distribution.

目的:调查荆州市气传真菌的种类、数量变化及飘散规律,了解优势菌群与过敏性疾病的关系,为荆州市空气真菌过敏症的防治提供参考。方法:2007年2月1日至2008年1月31日,在荆州市设曝片点,每日换片,收集全年气传真菌样品,在显微镜下统计,定量获得每天及全年的真菌数量。结果:全年均可见真菌孢子,共收集真菌种类20多种,其中最常见的有链格孢、锈菌、黑粉菌等,其数量和季节、气候有关,以春季、夏季最多,秋季和冬季依次降低。结论:气传真菌全年均有分布并具有较强的致敏性,部分真菌具有明显的季节性变化。

The other cells showed pleomorphic nuclei pyknosis, vacuolization showed symptoms, small size, with rows or clusters, sometimes we can see some acini.

另一类细胞则呈多形性,细胞核固缩,呈空泡状,体积小,成排或成簇排列,有时可见一些腺泡。

It was conclude that wind and weevils were effective pollination vectors for C. elongata, which inconsistent that Zamiaceae was host-specific insect pollination.

可见,风媒和虫媒都是越南篦齿苏铁的有效传粉途径,与泽米铁类植物为专一寄主性昆虫传粉的结论不一致。

The ultrasonogram characteristic of CHU was just like honeycomb anechoic opaca. There was no obvious blood signal in all the angiomas, but rich blood flow just like limb could be seen around the lesion.

特征性声像图表现为蜂窝状无回声暗区,CDFI其内未见明显血流信号显示,边缘可见丰富的树枝状血流。

Toner for the adhesive coated paper is poor in the bend of spine often visible ink on the paper avulsing.

墨粉对于涂布纸的粘着性较差,在书脊的转折处常常可见墨粉剥离。

It follows that the compartmentalized ion distribution on the organ level is one of salt-tolerance mechanisms in cucumber plants.

可见,离子在器官水平的区域化分布是黄瓜植株耐盐性的重要机理之一。

So you can see that teaching subjects with inter-subjectivity are not nominated but conformed to the teaching practice.

可见,有主体间性的教学主体不是&委任的&,而是在教学实践中确证的。

As exposed to optics microscope and inverted microscope,compared to control,SW480 cells with Giemsa staining that treated with the H2RA(cimetidine、nizatidine) took on malignance declining as crimpled firstly,cellular bulk and heteromorphism dimimished,the amount of rounded cell manifolded,nucleolus bulk lessened,dyeing thin,the amount of nucleolus decreased,partial cell organs manifest side-gathering phenomenon on chromatin,and the link between SW480 cell is loosened , subsequently partial rounded cell is floated.When the H2RA(cimetidine 40μM、nizatidine 20μM) against SW480 cells after 3 day,SW480 cells change roundly,nucleolus smashed,shrinking nucleolus came into being,and part of those not adhibited cell wall.

在光镜和倒置相差显微镜下,与对照组相比,经姬姆萨染色观察,西咪替丁、尼扎替丁处理的SW480细胞,先发生皱缩,体积变小,异形性减少,细胞变圆数目增多,细胞核变小,染色变淡,核仁数量减少,部分可见染色质边集现象;细胞间连接疏松,随后部分变圆细胞浮起。40μM西咪替丁及20μM尼扎替丁连续处理3天后,SW480细胞全部变圆,细胞核碎裂,固缩微核形成,部分不再贴壁。

Pathohistologic examination revealed normal glomeruli but vacuolar degeneration of tubular epithelial cells, and part of them disrupted and desquamated, and also tubular dilatation. Only mild pathological changes were seen in the intervention groups.

肾脏病理检查可见LPS组肾小球大致正常,而肾小管上皮细胞空泡变性,部分上皮细胞崩解、脱落,管腔扩张,两个UT干预组肾小管病理变化均较LPS组减轻,不同剂量组间形态差异无显著性。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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