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After treatment of benzamide which is an inhibitor of poly polymerase,β-galactosidase activity assays and PCR analysis were performed to test the deletion of LacZ gene and c-Ha-T24ras gene. The results showed that:(1)LacZ gene was deleted from both A13.4-NIH3T3 cells and B12.7-NIH3T3 cells, but neigher from A13.4-HeLa cells nor B12.7-HeLa cells. So it is possible that there is cell line specificity in gene deletion induced by BA.(2) In A13.4-NIH3T3 cells, LacZ gene and c-Ha-T24ras gene were completely deleted at the same time.(3) The transcription activity of exgenous DNA fragment may have same effect on its sensitivity to the deletion induced by BA.

用聚ADP核糖聚合酶的NAD位点抑制剂苯甲酰胺分别处理四种转化细胞后,检测细胞中整合的外源LacZ基因与c-Ha-T24ras基因的删除情况,结果如下:BA诱导的基因删除可发生于NIH3T3转化细胞系,但不能发生于HeLa转化细胞系;位于同一外源表达载体上的LacZ基因与c-Ha-T24ras基因的删除过程是同步的;外源整合DNA片段的转录强度可能直接影响其被BA诱导删除的敏感性。

Should remembering only is Pass By Value, can make clear Hunan actually, not necessary whole so complex I still feel to distinguish them cry betterer, what you say is right also, it is to pass a cost, but a copy that those who deliver is a value, another delivers the address that is area of cost seat memory, give this kind of distinction for expression, used in the program the value is delivered and cite deliver; I think the rock-bottom implementation of Java language also is C language; The habit that still is individual view is different, the understanding that is this value is different, if you consider as byte information so but choose a site for the capital is same.

只要记住都是pass by value,其实就可以搞清楚了,没必要整那么复杂我还是觉得把他们区别开叫更好一些,你说的也对,都是传值,但是一个传递的是值的拷贝,另一个传递的是值所在内存区域的地址,为了表达出这种区别,程序中使用了值传递和引用传递;我想java语言的底层实现也是C语言;还是个人看法的习惯不同,就是这个值的理解不同,如果你认为是字节信息那么可定都是一样的。

Introduced Fan Boolean Algebra theory as a whole, after analyzing and studying of it, concluded that: the characteristic of Fan Boolean Algebra has determined that it can solve the extant problem of Data Mining to a certain extent, for example, it can guarantee the result after data mining to be usable , assured and construable;can solve the problems about expression difficulty of complicated concept, correlation of attributes emphasized incompletely and redundant examining;it can set up the unified model of Fan Boolean in a certain system;and it can promote the developmental research of new decision support system.

对泛布尔代数进行总体介绍,在分析和研究它的理论体系后,概括出:它的特性决定了它能在一定程度上解决数据挖掘的现存问题,比如可以保证数据挖掘结果的可用性、确定性及可解释性,可以解决复杂概念表达困难、属性间的相互关系强调不够、重复检验等问题;能就某一系统建立统一的泛布尔模型;并促进新型决策支持系统的开发研究,并对泛布尔代数与数据挖掘相结合的原理展开论述。

Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

利用蛋白质结构同源建模工具,分别以人丁酰胆碱酯酶(1p0i:A)和果蝇乙酰胆碱酯酶(1d×4:A)的蛋白晶体结构为模板,对嗜虫书虱2个AChE的三维结构进行同源建模,并在三维结构中发现了AChE的酶解活性位点,证明嗜虫书虱体内也存在2个AChE基因。2.2嗜虫书虱β-actin基因克隆及乙酰胆碱酯酶基因mRNA表达水平研究目前关于嗜虫书虱的分子生物学研究较少,在GenBank中没有可用作内参基因的序列,因此本研究从嗜虫书虱体内克隆获得β—actin基因片段(GenBank登录号:FJ041117),该片段长度为822 bp,编码273个氨基酸残基,同源性比对分析表明该片段与其它昆虫的β—actin基因具有很高的同源性。

Type 2 diabetic patients show lower transcription of adiponectin mRNA in omental adipose tissue than that of non-diabetic control.

2 型糖尿病患者大网膜脂肪组织脂联素 mRNA 达显著降低,其表达水平可以作为胰岛素抵抗的重要参数;WC、TG、FBG、WHR、病程等临床指标,可间接反应 IR 的程度,而且以 WC 的参考意义最大;糖尿病的病程尤其是 5 年以上的病程也可以作为 IR 的一个重要参考指标。

Hewever the caspase-3 expression was still very high in cortex. Conclusion: In KA model the hippocampus amygdala plays a key role in epilepsy. Therefore it provides a mimic optimal conditions for human temporal lobe epilepsy study. Its an effective method of studying the development, change and pathopoiesis of epilepsy. There is a close relationship between NPY and epilepsy. The regulation of NPY via VPA may be one of the antiepilepsy mechanism of mechanism of VPA. Moreover NGF can enhance the caspase-3 expression. Excito-amino acid can aggravate the apoptosis, which may be one of the reason for intractability of epilepsy.

KA模型因其海马杏仁核在癫痈中起核心作用,故具有模拟人类颗叶癫痈的理想条件,是研究癫痈发展变化、致痈机理的有效工具,NPY与癫病有着密切的联系,VPA调节NPY有可能是其抗癫痈机理之一,而NGF对caspase一3表达有促进作用,兴奋性氨基酸可造成细胞凋亡的增加,而凋亡细胞的增多有可能是癫痈久治不愈的原因之一。

The general decoding method for Turbo codes provided by the invention adopts enhanced Max-Log-MAP algorithm, makes use of Max-Sum semiring property in the generalized distribution law and combines and simplifies measure and calculation of forward and backward status of traditional binary Turbo codes, which means combining grid graph of traditional binary Turbo codes in nature to ensure that the status measure and calculation of the traditional binary Turbo codes have the same calculation expression way with that of the duobinary Turbo codes so that a general decoding device can both decode the traditional binary Turbo codes and duobinary Turbo codes.

本发明提出的Turbo码通用译码方法采用增强型Max-Log-MAP算法,利用广义分配率中的Max-Sum半环性质,对传统二进制Turbo码的前向和后向状态度量计算进行合并简化,从本质上也就是对传统二进制Turbo码的网格图进行合并,使得传统二进制Turbo码的状态度量具有和双二进制Turbo码一样的计算表达方式,从而使得通用译码装置对于传统二进制Turbo码和双二进制Turbo码都可实现。

Results In the follicles of estrous cycle rat,the signals of MMP 2mRNA were detected in the granulosa cells and thecal cells;w...

结果 在动情周期中,各情期卵巢中卵泡的颗粒细胞和发育良好的卵泡的膜细胞中均可检测到MMP 2mRNA的杂交信号;MMP 9mRNA的表达始于已发育良好的窦前卵泡的膜细胞,此后在小窦状卵泡、窦状卵泡及排卵前卵泡的膜细胞中均检测到MMP 9mRNA的杂交信号。

A highly conserved sequence of 210 bp of AtNHX1 gene that encodes a Na+/H+ antiport protein was isolated from A.thaliana,the 210 bp fragment was amplified as RNAi target site and used in construction of RNAi vector,and was inserted into pHANNIBAL as reverted repeat with an intron.The expressional box was linked into the vector of pART27,NPTⅡgene is the screening marker in this vector.

利用RNAi研究植物基因功能,可选择靶基因的一段单一序列使基因家族中的单个成员沉默;也可以选择某一基因家族的多个成员具有的一段同源性很高的保守序列,设计针对这一序列的dsRNA表达载体使多个基因同时沉默,RNAi成为功能基因组学研究的重要手段。

This mexican movie is not just about romantic love; it"s alsoabout love for food, family, and tradition. pedro and tita can"t getmarried because titas mother wants her oldest daughter to be marriedfirst – to pedro. and she wants tita to stay home and take care of her.tita acquiesces, but finds that her cooking has unusual powers.

这部墨西哥电影可不止是简单的爱情故事,它同时表达了对食物、对家庭和对传统的热爱:pedro和tita相爱却不能结婚,因为tita的母亲希望自己的长女能嫁给pedro,而希望tita能留在家中照顾自己。tita勉强答应了母亲的要求,同时发现自己的烹饪有些特殊的能力

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。