可表达的

With the optimization of the cultivated conditions, the recombinant fusion protein staphylokinase-hirudin was over-expressed in E.coli BL21(DE3), and the final cell density reach 115g wet cell weight per liter, the target protein is about 1.1~1.2g/L accounting for more than 30% of the total protein. The culture process in 5L fermentor was performed on 40L fermentor successfully, and indicated that the process was easy scalable.

通过对培养条件的不断优化，重组葡激酶-水蛭素融合蛋白在大肠杆菌BL21(DE3)里得到了高效表达，菌体密度最终达到115g/L以上，可溶性重组融合蛋白占菌体总蛋白的30%以上，含量约为1.1~1.2g/L.5L发酵罐的发酵工艺参数在40L发酵罐中进行了放大培养，结果表明该工艺能有效的放大，可适用于工业生产。

In accordance with the present invention, optically pure-hydroxycarboxylic acid can be prepared by culturing E. coli transformed with a recombinant plasmid which expresses intracellular PHA depolymerase of Ralstonia eutropha and PHA biosynthesis enzyme of Alcaligenes latus to give-hydroxycarboxylic acid.

根据本发明，光学纯的-羟基羧酸可通过培养用重组质粒转化的E.coli制备，所述重组质粒表达真养产碱杆菌的胞内PHA解聚酶和广泛产碱菌的PHA生物合成酶从而生成-羟基羧酸。

Recombinant adenovirus was identified by polymerase chain reColon cancer cell line SW480 was infected with recombinant adenovirus Ad-p27mt, and expression of p27 protein was detected by Western blot.of expressed p27 protein after Ad-p27mt transfected colon cancer cell.novirus framework plasmid pAdeasy-1 with pShuttle-CMV-hp27mt, 30％combinant adenovirus DNA contained the target gene.

Ad-p27mt的聚合酶链反应检测Ad-p27mt转染大肠癌细胞后的p27mt的表达。结果：①用含pShuttle-CMV-hp27mt转化含pAdeasy-1的超感受态BJ5183后，可获得约30％的阳性重组质粒。②聚合酶链反应检测表明重组腺病毒DNA中含有目的基因。重组腺病毒滴度为7.95×1015pfu/L。

Our results showed that both uterine CD63 mRNA and protein levels were highly expressed at implantation sites on day 5 of pregnancy,and CD63 mRNA signal was strongly detected in the decidual zone on the following days 6-8 of pregnancy.

CD63的mRNA和蛋白水平在妊娠第5天子宫的着床位点均显著上调，在随后第6-8天的蜕膜区，均可检测到明显的CD63 mRNA表达信号。

Experimental results show that the proposed method can be used to prepare high titer and specific rabbit anti-Humulus pollen protein polyclonal antibody. It has similar ability in determining the composition of Humulus pollen allergic protein compared with sera of patients. Therefore, it can be used to immunoscreen cDNA library.

制备的兔抗血清具有较高效价和较好的特异性，应用该血清筛选出的葎草花粉致敏蛋白组分与患者血清筛选的组分相似，因此可进一步应用于初筛cDNA表达文库。

In addition, the specific POD band (Rf = 0.67) of C. hytivus and C. hystrix was detected in each individual of population, which indicated this band could be a good biochemical marker for monitoring the flow of germ plasm from C.

另外， 新种与野生种的特征性POD酶带(Rf=0.67)在群体内各单株内都有表达，这表明该带可作为一个稳定的生化标记在监控新种的遗传物质向栽培黄瓜的流动中发挥作用。

From the computed results, the following preliminary conclusions can be drawn: the time domain analysis of the buffeting response is feasible by using the indicial function formulae of the self—exciting force; usually, the linear analysis method will overestimate the buffeting response.

由本文的计算结果还可得出如下初步结论：利用自激力的阶跃函数表达法对抖振响应进行时域分析是可行的；通常的线性分析方法将偏大地估计抖振响应。

Results The expression of the Mahoganoid protein and its mRNA was showed by brown staining and observed in the Leydig cells, spermatogonia, primary spermatocytes, spermatids, Sertoli cells, and peritubular myoid cells of testicular tissues and principal cells, basic cells and epithelial cells of epididymal tissues.

结果1。免疫组化法检测Mahoganoid蛋白的实验结果显示的棕色阳性信号可见于各天龄段大鼠睾丸的生精细胞、支持细胞、间质细胞和管周肌样细胞以及附睾输出管的高柱状、低柱状上皮细胞和附睾管的上皮细胞、主细胞、基细胞。其中Mahoganoid蛋白主要表达于胞膜和胞浆。

Expression analyses identified increased expression of numerous TGF-beta-related genes that regulate cell proliferation and survival and plausibly contribute to myxomatous valve disease.

他们注意到，多数效应器分子的表达增加降低了TGF-β的信号作用，包括可诱导TGF-β的H3基因、内皮素-1、组织抑制因子金属蛋白酶1、致骨形态学发生的蛋白和&调节细胞增生和存活可能还有促进粘液瘤性疾病的基因&。

As it known, mistakes in spatiotemporal expression of critical genes result in congenital cardiovascular diseases. Furthermore, studies on high risk population of cardiovascular disease have also suggested the relationship between noncongenital cardiovascular diseases and genetic factors.

在心血管系统疾病的研究中已发现，某些基因在时空上的表达紊乱可导致先天性心脏病，而对部分心血管系统病症高发人群的研究也证明部分后天形成的心脏病与遗传因素有关。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐