可表达的

It was demonstrated that the length of the 45m gene was 698 bps, coding for 232 amino acids.

表达的重组蛋白分子量约为63kD，表达产量可达100~120mg/L。

By the principle of Verification logical positivism drives problems of value out of philosophy.

逻辑实证主义根据"可证实性原则"斥逐价值问题；杜威在认可"可证实性原则"的前提下，从"价值表达的可证实性"、"情感、欲望的可证实性"、"评价判断的可证实性"三个方面证明了价值判断的可证实性，从而捍卫了哲学研究价值问题的合法性。

Pronucleus expression recombinant pMAL-C2X-HBV-X is constructed successfully and with the IPTG induction, the recombinant pMAL-C2X-HBV-X can express the X protein in E. coli JM109, which lays the foundation fur the HBV X protein purification and its biological study.

成功构建了HBV X基因原核表达重组体pMAL-C2X-HBV X；在IPTG的诱导下，该重组体在大肠杆菌中可表达X融合蛋白，为进一步纯化HBV X蛋白和研究其生物学作用奠定了基础。

HCV NS3C/NS4 protein fused with E. coli thioredoxin was expressed insolubly although thioredoxin is a highly soluble fusion partner. NS5B-dc21 protein was soluble, which is a truncated version of NS5B, while NS5AB protein was insoluble, which needed resolubilization and refold procedures.

HCV NS3C/NS4蛋白是以硫氧还蛋白融合表达的方式获得的，尽管硫氧还蛋白有很强的助溶作用，该蛋白依然不可溶形式存在；NS5B—dc21蛋白通过C末端疏水序列的删除，获得了可溶表达；NS5AB蛋白以不可溶形式表达，需要复性。

After 72 h induced by Progesterone and Glucocorticoid as well as Insuline,biological activities of IL-2 were assayed with morphological docimasia method.

结果显示，转染细胞均可表达IL-2，且所表达的IL-2有促进T淋巴细胞转化和淋巴母细胞成熟的活性；转染pcDNA3 OVP—IL2的输卵管上皮细胞表达产物在1：256稀释水平下仍具有促进T淋巴细胞转化和淋巴母细胞成熟的活性。

After 72h induced by Progesterone and Glucocorticoid as well as Insuline, biological activities of IL-2 were assayed with morphological docimasia method. The results showed that the plasmids of pcDNA3-QVP-IL2 and pcDNA3-IL2 could express IL-2, and the IL-2 could augment T cell proliferation.

结果显示，转染细胞均可表达IL-2，且所表达的IL-2有促进T淋巴细胞转化和淋巴母细胞成熟的活性；转染pcDNA3-OVP-IL2的输卵管上皮细胞表达产物在1：256稀释水平下仍具有促进T淋巴细胞转化和淋巴母细胞成熟的活性。

There is Cx43 expression in the human thyroid epithelial cells, and there are heterogenicity in levels of Cx43 expression in autoimmune thyroid diseases.

Cx43可在人甲状腺上皮细胞中表达；Cx43在AITD的表达强度存在异质性，这种缝隙连接蛋白表达的异质性可能与AITD的发生、发展及预后有关。

It was more differentiated in coculture group than inconditioned group. MSCs and myocytes could form gap junction when cocultured in vitro.Conclusions: MSCs could survive and develop in normal myocardium.

正常MSCs中部分细胞可表达少量Cx43以及titin；条件培养基和混合培养均可促进MSCs的心肌样分化，但后者使分化程度更高；混合培养条件下，MSCs可和心肌细胞之间形成缝隙连接。

Objective: The purposes of the present study are as follows: 1 To study the effect of direct contact with VSMC on transdifferentiation of MSC; 2 To construct MSC of Dual-stable expression of AT2R gene medicated by tetracycline regulatable system.; 3 To investigate the effects of AT2R gene medicated by tetracycline regulatable system on neointimal hyperplasia in rat carotid arteries after balloon angioplasty.

本实验体外观察MSC与VSMC在直接接触培养下的分化情况，并以MSC为载体，通过体外细胞基因转染及筛选，获得双重稳定受到强力霉素调控表达AT2R基因的MSC，将此受到调控表达的AT2R基因导入大鼠颈动脉球囊损伤动物模型中，观察MSC细胞移植实现AT2R基因在体可调控表达在新生内膜中形成的作用及潜在意义。

The dynamic changes of the the neuron apoptosis and correlative genes in hippocampus of ACET-groups and twice-ACET-groups suggested that ACET can slower the apoptosis by inhibiting p~(53)and prolong the ascending period of p~(53) in 4 days after the seizure,this effect would weaken after 4 days.ACET may inhibit the descending of Bax,prolong its descending period and then promote its expression,in order to weaken the inhibition of the apoptosis during 4 to 10 days after seizure and then promote the apoptosis during 10 to 14 days after.This effect of ACET can be enhanced after treating again within 7 to 14 days later.

埋线组和二次埋线组大鼠海马神经元凋亡及相关基因表达的动态变化结果提示：埋线治疗在癫痫发作后的早期(4d内)可通过抑制促凋亡基因p~(53)蛋白表达、延缓其达峰值的时程，从而减缓神经元凋亡的发生，此作用在4d后减弱；埋线治疗抑制大鼠海马神经元凋亡的作用在癫痫发作后的中期(4d～10d)减弱，甚至在远期(10d～14d)出现促进凋亡的作用，可能是通过抑制Bax蛋白表达的下降、延长其降至低峰值的时程，促进Bax蛋白表达的回升的结果；在中远期(7d～14d)内予二次埋线治疗对该效应有续效叠加的作用。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐