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And large numbers of mutant p53 protein were expressed in liver, kidney, ventriculus glandularis, tumor, oviduct. Moderate expression were detected in heart, pancreas, bone marrow, spleen, lung, duodenum, thymus and bursa of fabricius.

结果表明:突变型p53在肝脏、肾脏、腺胃、肿瘤、输卵管中表达量最高,心脏、胰脏、骨髓、脾脏、肺脏、十二指肠、胸腺及法氏囊均可检出中等量的表达。

Bi-allelic or biparental expression of alleles may be present in some tissues and uniparental expression in other tissues.

某些组织中可能出现等位基因的双等位基因或双亲表达,其他组织中是单亲表达。angelman综合征和prader-willi综合征都可因第15号染色体缺失而产生。

By using immunohistochemical technology and image analysis system and laser confocal imaging system anlysis,the level of HBcAg endocellular expressed dropped down.

ELISA方法检测发现,核酶抑制HBeAg表达50%~65%,用免疫荧光、激光共聚焦分析,免疫组化等均证实核酶可抑制HBV细胞内的表达。

The results show that mica can reduce gastric mucosa inflammation cell infiltration and glandulose atrophy, increase the positive expression of glandulose nuclear proliferation antigen and raise the expression level of blood serum epidermal growth factor.

结果发现云母使胃粘膜炎症细胞浸润、腺体萎缩程度减轻,腺体PCNA阳性表达增加,并可增加血清EGF的表达水平。

PartⅡThe mechanism elucidation about effects ofα-(2,3)/(2,6)sialic acid on the Cx43gap-junction functions.(1) Westernblotting experiment showed that the decrease of sialic acid didn\'t changethe Cx43 expression and its phospholation level.(2) Westernblotting experiment showed sialidase didn,t change the ZO-1 expression,IP and confocal experiment showed sialidase improved the interaction of Cx43 andZO-1.(3) Westernblotting experiment showed sialidase didn\'t change N-cadherin expression,IP and confocal experiment showed sialidase promoted the complex formation ofCx43 and N-Cadherin.(4)Sialidase could increase the ERK1/2 phospholation level,and enchanedintercellular homotypic adhesion,Immunofluorometric assay showed sialidase couldpromote the N-cadherin cluster on the membrance.

第二部分:α—(2,3)/(2,6)唾液酸对肿瘤细胞CX43间隙连接功能影响的机制研究1、Westernblotting结果表明降低细胞膜表面唾液酸并不改变Cx43的表达及其磷酸化水平:但Cx43连接斑形成增多;2、Westernblotting结果表明唾液酸酶作用后细胞ZO—1表达没有改变,IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与ZO-1的结合;3、Westernblotting结果表明唾液酸酶作用后细胞N-cadherin表达没有改变,IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与N-Cadherin复合物的形成;4、唾液酸酶作用后细胞内ERK1/2磷酸化水平明显增加,细胞间同质粘附增加,以及免疫荧光表明唾液酸降低后可促进N-cadherin的膜成簇。

Results HE staining showed that sulindac could inhibit the growth of tumor cells, with the morphological presentations as apoptosis and necrosis including pyknosis, karyorrhexis and chromatin crescent formation, etc. By electron microscopy, cells also had apoptotic characteristics including chromatin crescent formation and apoptotic body. Immunohistochemistry and Western-blot assay results showed that 48 hours after sulindac administration, the expression of NF-κB, bcl-2 and bax gradually decreased, while the level of bax protein gradually increased, in a dose dependence manner.

结果 苏木精-伊红染色检测结果显示舒林酸抑制肿瘤细胞生长,肿瘤细胞出现核固缩、核碎裂、染色质边集等凋亡及坏死的形态学表现;电镜下可看到染色质边集、凋亡小体;免疫组化、Western-blot结果显示舒林酸作用48 h后人胃癌SGC-7901细胞NF-κB、bcl-2表达逐渐下降,bax表达逐渐上升,具有剂量依赖性。

Micrococcal lysis assay of the milk samples showed that the cDNA was efficiently expressed in the mammary glands with similar expression levels ranging from 60 to 87 mg/L. Different tissues of each vector-injected mouse were assayed for transcription of hLYZ mRNA by dot-blotting and for expression of hLYZ activity by Micrococcal lysis assay. The results showed that expression of the three recombinant vectors was mainly restricted to mammary glands with some degrees of ectopic expression in spleen, intestines and/or kidney.

注射后72 h采集乳样,经微球菌溶解实验证明,3组小鼠乳汁中hLYZ的表达量分别为87、69、60 mg/L;从每组小鼠的12种组织提取总RNA,经Dot blotting检测证明,三种重组载体除在乳腺中表达外,p205C3LYZ还在小鼠的脾和肠、pBJLYZ在脾、pBCLYZ在脾和肾中具有一定的异位表达;在有hLYZ mRNA转录的上述脏器中用微球菌溶解试验均可检测到hLYZ活性。

To construct a plasmid for analyzing gene functions and expressions and to study the MXAN1334 gene in Myxococcus xanthus with the plasmid.

旨在构建一个用于基因插入失活,并同时可通过报告基因分析插入位点基因表达情况的质粒载体,并应用该质粒对黄色粘球菌MXAN1334基因功能和表达情况进行分析。

Moreoer, c-Fos and phospho–c-Jun protein expression was inhibited by PPARα agonists, indicating that PPARα ligands suppress OPN expression through negatie cross talk with AP-1–dependent transactiation of the OPN promoter.

另外,c-Fos及磷酸化c-Jun蛋白表达可被PPARα激动剂抑制,说明PPARα配体通过OPN启动子AP-1依赖型转录激活的负交互作用而抑制OPN表达。

The distribution of UEA-1 receptors in pseudopregnant rabbit uterus was similar to that of normal pregnant rabbit uterus.

注射雌激素可明显促进卵巢切除后兔子宫内膜UEA-1受体的表达,而注射孕酮对其表达有明显抑制作用。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

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