可表达的

HSP90 as a important member of HSP protein family, are usually constitutively expressed in most mammalian cell types and can be further induced by heat shock and other stresses.

HSP90是热激蛋白家族的主要成员之一，它在细胞中呈高组成性表达，热激和其它应激条件可进一步诱导其表达。

The colony forming and the cell cycle were analyzed with flow cytometry, and the mRNA expression of bcl-2, c-myc gene were determined with RT-PCR method. Results Resveratrol could markedly inhibit proliferation of human gastric cancer cells compared with that of the blank control group. The cell cycle was blocked to G0/G1. The mRNA level of bcl-2 and c-myc gene were decreased compared with that of the blank control group.

结果 白藜芦醇可抑制胃癌细胞增殖和集落形成能力，低、高剂量组的抑制率分别为6.9%，17.6%和27.98%，63.39%，差异均有统计学意义(P.05)；白藜芦醇能使胃癌细胞周期阻滞于G0/G1期，明显下调胃癌细胞bcl-2、c-myc基因mRNA表达水平(P.05)；用药24 h后，白藜芦醇高剂量组bcl-2和c-myc基因相对表达量分别为(0.54±0.04)，(0.58±0.05)，与空白对照组(0.91±0.05)，(0.85±0.03)比较，差异均有统计学意义(P.05)。

Except for 2μmol/L Verapamil,other concentrations ofCAs and CaMAs could decrease mdr1 mRNA expression level of K562/VCRcells,indicating significant difference from those of control group(CAs:P＜0.05;CaMAs:P＜0.01),but the changes of mdr1 mRNA expression levelcaused by CaMAs had negative dose-responses relationship with CaMAsconcentration(P＜0.05),while the changes of mdr1 mRNA expression levelinduced with CAs had no dose-responses relationship with the CAsconcentration.

除〓的Ver外，其它浓度的CAs和CaMAs均可降低K562/VCR细胞mdr1 mRNA表达水平，与对照组相比，存在着明显差异（CAs：P＜0.05；CaMAs：P＜0.01）；并且CaMAs各浓度间引起的mdr1 mRNA表达水平的变化也存在着差异（P＜0.05），呈负的量效关系，而CAs各浓度间引起的mdr1 mRNA表达水平的变化无明显差异。

H. "Work" means the literary and/or artistic work offered under the terms of this License including without limitation any production in the literary, scientific and artistic domain, whatever may be the mode or form of its expression including digital form, such as a book, pamphlet and other writing; a lecture, address, sermon or other work of the same nature; a dramatic or dramatico-musical work; a choreographic work or entertainment in dumb show; a musical composition with or without words; a cinematographic work to which are assimilated works expressed by a process analogous to cinematography; a work of drawing, painting, architecture, sculpture, engraving or lithography; a photographic work to which are assimilated works expressed by a process analogous to photography; a work of applied art; an illustration, map, plan, sketch or three-dimensional work relative to geography, topography, architecture or science; a performance; a broadcast; a phonogram; a compilation of data to the extent it is protected as a copyrightable work; or a work performed by a variety or circus performer to the extent it is not otherwise considered a literary or artistic work.

h。 「本著作」指本授权条款下提供的文学及艺术著作，包含但不限於以任何方式或形式表达之文学、科学及艺术范围之制作物，例如：书籍、小手册及其他撰著；演讲、演说、布道及其他类似同性质之著作；戏剧著作或歌剧著作；舞蹈著作或默剧；含歌词或不含歌词之音乐作曲；电影著作及其他藉由与电影摄影技术类似之程序表达之同类著作；素描、绘画、建筑、雕塑、雕刻或版画之著作；摄影著作及其他藉由与摄影技术类似之程序表达之同类著作；应用艺术著作；插图、地图、设计图、素描及地理、地形、建筑或科学相关立体著作；表演；广播；录音物；和可受著作权保护的著作同样受保护的资料编辑物；或种种表演人或马戏团表演人表演的不被视为文学或艺术著作的著作。

The mRNA and protein expression level of Bax in rats experimental cryptorchidism increases significantly.

隐睾生精细胞Bax表达增加，L-NAME可通过下调Bax的表达抑制隐睾生精细胞凋亡。

The identity and difference in the expressional patterns between distribution functions of discrete random variables and distribution functions of continuous random variables are elaborated,and the main expressional patterns of distribution functions of continuous random variables are summarized,and the derivability of distribution functions of continuous random variables is discussed.

0引言在概率论中，连续型随机变量分布函数的表达形式及其可导性是一难点。其一，离散型随机变量的分布函数有较明显的性质和统一的表达形式，而连续型随机变量，由于其概率密度函数一般为分段函数，根据其分段区间的差异，表达形式差异较大，不容易总结其规律，本文主要比较连续型随机

By in situ hybridization, 53BP2 mRNA expression is revealed in tumor cell nests of NPC paraffin sections. The strong positive particles localize in the nuclei of tumor cells; but slight positive signals are also found in tissue cells of chronic nasopharyngitis and connective tissue, such as lymphocytes and smooth muscle cells; There are also positive signals in the nuclei of hyperplastic squamous epithelial cells. However, 53BP1 mRNA expression is not found in nuclei of tumor cells, tissues of chronic nasopharyngitis and connective tissue.

原位杂交结果显示，石蜡切片的鼻咽癌癌巢内，可见53BP2的强阳性表达信号，信号呈紫色颗粒状分布于胞核，核仁清晰可见；而在相同条件下，在慢性鼻咽炎组织和癌旁上皮的结缔组织，也可见弱表达信号，如淋巴细胞和平滑肌细胞；在增生的鳞状上皮细胞的核内，可找到强阳性信号。53BP1在鼻咽癌、慢性鼻咽炎组织和癌旁上皮的结缔组织切片上，均无表达信号。

The results suggest that administration of sodium salicylate can decrease the expression of NGB mRNA and protein of spiral ganglion cells in guinea pig and these changes are relative with the dosage of sodium salicylate.

水杨酸钠可显著降低螺旋神经节神经元NGB mRNA和蛋白表达；水杨酸钠对螺旋神经节神经元NGB mRNA和蛋白表达是剂量依赖性的。

Each medicine used in the study totally has obivious effect on lens sodium-pump. Either on the molecular and cellular level or on the histological and organic level , they all have their distinctive demonstration. 1.Ouabain、Digoxine、DMSO decrease the expression of αsubunit of sodium pump on mRNA level , the cell morphology and ultramicroscopic structure demonstrated destructive effect,it indicates the cause of cataract of the medicines.2.D-thyroxine、Amphotericin B、Vitamin E increase the expression of αsubunit of sodium pump on mRNA level ,from the demonstration of the cell morphology and ultramicroscopic structure ,it indicates the medicines have protective or anti-distructive effect on lens.3.The medicines have isoform-specific action on lens sodium-pump,it indicates exploitting isoform-specific medicine has a promising clinical application.4. Lens sodium-pumps are inhibited probably lead to cataract, so in clinical medication, especially to the cardiovascular doctor, while he is using the sodium-pump inhibitors, he should pay more attention to adjust the dosage and duration of the drug therapy. 5. To creat the animal model of cataract through inhibiting lens sodium-pump, whether it can be a kind of classical method or not, it is worthy of further research.

本研究所选用的药物对晶状体钠泵均有明显的影响作用，在分子水平、细胞水平、组织器官水平均有特异的表现：1、哇巴因、地高辛、二甲基亚砜三种药物可降低晶状体钠泵α亚单位在 mRNA水平的表达，在细胞形态和超微结构上表现为损伤效应，提示了其潜在的导致白内障的可能。2、D甲状腺素、两性霉素B、维生素 E三种药物可升高晶状体钠泵α亚单位在 mRNA水平的表达，从细胞形态和超微结构上的表现，提示了其对晶状体有保护作用或抗损伤效应。3、药物作用于晶状体钠泵具有重整异构特异性，提示了开发出特异的具有重整异构作用的药物是极有临床应用前景的。4晶状体钠泵抑制后可能导致白内障的发生，故临床用药，尤其是心内科医生在使用这些钠泵抑制剂时应注意调整用药剂量及药物疗程。5 通过抑制晶状体钠泵来制作白内障动物模型能否成为一种经典的制作方法，还有待于进一步研究。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。