可表达的

This protein reduced antibody titer in mice immunized with BSA. Following the deletion of sCD152, however, there was no obvious inhibitory effect on the primary immune response triggered by the nonrelevant antigen OVA, indicating that sCD152 had an antigen-specific immunosuppressive effect. We designed a new vector, pPIC-HE-sCD152, to get entirely native sCD152 without His-tag.

为了获得既带有纯化配体表位又带有可剪切序列的可溶性sCD152蛋白，我们设计构建了含有His-tag和肠激酶识别切割序列DDDDK的sCD152表达载体pPIC-HE-sCD152，结果这一表达载体在毕赤氏酵母中同样高效分泌表达出目的蛋白，大量纯化后用肠激酶酶切去除His-tag，用以进一步功能实验，初步结果表明具有体外细胞免疫抑制功能。

Results Expression of GFP was not detected in control group, and was detected in peritoneum of transfection group at all time points.

结果 正常对照组的小鼠腹膜组织未能检测到GFP表达，而PAMAM G9/pHK-shRNA转染组的各时间点均可检测到GFP表达，以48h表达最强，显著高于其他各时间点(P＜0.01)。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

However it was shown its eventually inhibiting effect on apoptotic myocyte cells during I〓 R〓h (P＜0.05); The PKC inhibitors chelerythrine and scutellarein have no effect on expression of Fas protein, whereas they can significantly upregulate the expression of Bcl-2 protein.

4PKC激活剂PMA可下调Fas基因的蛋白表达，对Bcl-2蛋白的表达也有抑制作用，它对缺血30min、再灌注24h后心肌凋亡细胞的最终影响为抑制效应(P＜0.05)，而PKC抑制剂CHE、灯盏花素对Fas基因蛋白表达无明显干预影响，但对Bcl-2蛋白表达呈显著上调作用，故对心肌细胞凋亡的抑制作用更为明显(P＜0.01)。

Furthermore, knockdown of Hspa5 rescues ventralized phenotypes induced by overexpression of Bmp2b; on the other hand, dorsalized phenotypes caused by Hspa5 knockdown disappear when Bmp2b mRNA is coinjected. It is likely that involvement of Hspa5 in dorsoventral patterning is associated with Bmp signals.

进一步研究发现，抑制Hspa5的表达能够挽救Bmp2b过量表达所导致的胚胎腹部化，Bmp2b的过量表达可使阻断Hspa5表达所引起的背部化表型减弱，提示Hspa5在胚胎背腹分化方面的作用可能与Bmp信号相关。

In theory, on entry into endosome of tumor cells immunoGrB protein releases C-terminal PE Ⅱ-GrBa into cytosol as a result of auto-cleavage of peptide bond between Arg279 and Gly280. Two kinds of PE translocating peptides covering 253-364 aa and 253-358 aa were compared for their endosome-disruptive function.

一方面，从瞬时表达、可诱导表达和组成性表达三方面研究证明，N端部分PE Ⅱ序列(PE 280-364 aa和280-358 aa)的存在基本不影响GrBa诱导转染细胞死亡的作用。

Down-regulated expression of PTEN and up-regulated expression of VEGF〓 were considered as two important events in tumorigenesis of ovarian cancer and could act as molecular markers to indicate the pathobiological behaviors of ovarian cancer. Decreased PTEN expression and increased VEGF〓 expression were closely associated with tumorigenesis and pathobiological behaviors of ovarian endometrioid and serous cancer respectively. Reduced expression of PTEN gene might be involved in carcinogenesis and progression of ovarian cancer by up-regulating the VEGF〓 expression to enhance angiogenesis.

在卵巢癌组织中PTEN基因mRNA表达下调和VEGF〓基因mRNA表达上调是卵巢癌发生的重要分子事件，可作为反映卵巢癌病理生物学行为的指标，且分别与卵巢内膜样癌和浆液性囊腺癌的发生和病理生物学行为关系密切；PTEN基因mRNA表达降低可能通过上调VEGF〓基因mRNA表达促进新生血管形成，进而参与卵巢癌的发生和侵袭。

ResultsIFA showed the G1 and G2 genes were successfully expressed, and the fluorescent signal was robust and concentrated in the perinuclear region of the transfected cells.

结论成功构建了糖蛋白G1、G2的表达载体，并在Vero E6细胞中呈有效表达，二者的共表达可在偏酸性条件下引起Vero E6细胞发生融合。

In response to extracellular stimuli, ERK1/2 translocates to nucleus, phosphorylates transcription factors,such as Elk1,ATF2,Sap-1α,c-myc,STAT, and so on, co-regulators and chromatin proteins to initiate transcriptional changes.

斑点杂交研究发现Ngb mRNA在大脑皮层、海马和丘脑等部位表达，缺血缺氧可使Ngb mRNA表达上调，其表达上调程度与相应区域对缺血缺氧的耐受性呈正相关。

The expression of these two genes were different in the time after ischemia in different parts of brain.c-fos was mainly induced in hippocampus and cortex of brain,whereas HSP70 expressed predominantly in cortex and corpus striatum.

实验结果表明，局灶性脑缺血可诱导c-fos和HSP70的表达；因不同脑区对缺血的敏感度不同，c-fos和HSP70的表达程度及表达时间亦不尽相同，c-fos主要在海马和皮层中表达，而HSP70则主要出现在皮层和文体区。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。