可表达的

Result The inhibitory effect on pulmonary metastasis of transplanted tumor were 46.36%, 32.73%, 18.2% in the Runing granule preparation group, warming kidney group, dispersing liver and promoting blood circulation group respectively. There wasn＇t significant difference between Runing granule preparation group and CTX group （50.91%）. The expression of VEGF was dropped in Runing grannie preparation group and dispersing liver and promoting blood circulation group. The expression of TIMP-1mRNA was up-regulated in Runing Granule group and warming kidney group. The expression of MMP-9 was dropped in Runing granule preparation group and warming kidney group.

乳宁冲剂组及拆方温肾组、疏肝活血组对裸鼠乳腺癌移植瘤的肺转移抑制作用分别为46.36％、32.73％、18.2％，乳宁冲剂组作用与CTX组（50.91％）比较无显著差异；而乳宁冲剂组、疏肝活血组均可下调肿瘤VEGF蛋白表达；乳宁冲剂全方、温肾方可明显使TIMP-1mRNA的表达升高，MMP-9表达下降；对MMP-9／TIMP-1蛋白表达比值影响以温肾组下降最明显。

Methods In this study,a plasmid vector,rAd5,and rAAV2 were used to transfect rat cochlear marginal cells of stria vascularis by injection into the perilymph through the round window membrane,and the transfection efficiency, target tissue accessibility, cell/ tissue toxicity, time course of expression, and effect on hearing were evaluated in vivo and in vitro.

结果三种载体对原代培养的大鼠耳边缘细胞的转染研究发现，腺病毒的转染效率最高，而腺相关病毒转染对细胞活性影响最小。rAAV2和rAd5对大鼠耳蜗组织的感染实验发现，腺病毒和腺相关病毒携带的增强型绿色荧光蛋白可在多种耳蜗组织中及转染对侧耳蜗组织表达，且并不引起明显的耳蜗组织细胞凋亡。rAAV2携带的EGFP基因在第90天时仍可检测到大量表达。rAd5携带的EGFP基因在第30天时表达明显减弱。

Pem gene is expressed in a time and stage specific manner. Pem transcripts are detectable as early as day 6 in mouse embryonic development, and are abundant in day 7 and 8, but decrease precipitously thereafter. Although Pem transcripts are not detectable in most of adult tissues, they are present in reproductive system. Furthermore, Pem has also shown to be specifically expressed in the proximal cauda and distal corpus regions of the epididymis, the regions where spermatozoa gain forward motility and fertilization competence.

Pem基因的表达具有明显的时间和空间特异性：在鼠胚发育的第6天，即可检测到Pem转录本的表达；第7-8天，Pem表达量达到最高，随后迅速减少；Pem基因在成年组织中一般不表达，但可在生殖组织表达；另外，Pem特异地在附睾近侧尾部和远侧的体部中表达，此处是精子获得运动性和受精能力的地方。

IL-1βmRNA expression in the developmental brain in Wistar rat: IL-1β mRNA in hippocampus, cerebral cortex and subcortex was detected by postnatal day 1 and gradually increased with development, reached a peak at postnatal day 21, and then gradually decreased to lower level at adult; In P77PMC rat, IL-1β mRNA expression was similar to Wistar rat before postnatal day 14, then gradually increased and surpassed Wistar rat at postnatal day 28, reached a peak in adult.

原位杂交显示谷氨酸诱导Wistar大鼠惊厥和P77PMC大鼠听源性惊厥后脑内IL-1βmRNA表达情况。结果发现，两种不同惊厥模型大鼠惊厥后脑内IL-1βmRNA表达存在明显的差异。谷氨酸引起惊厥诱导脑内皮层、海马和丘脑IL-1βmRNA表达增高的程度远较P77PMC大鼠听源性惊厥为低，而且惊厥后IL-1βmRNA表达增加较慢，于4-8h达高峰，而听源性惊厥可于惊厥后2h迅速达高峰。但两种形式惊厥诱导脑内IL-1βmRNA表达最高的部位均是海马。

Flow cytometry results showed that: 1） When cells were treated with Diallyl trisulfide at the concentration of 10 μg/mL for 12 h and 24 h the percentage of G0/G1 phase cells was decreased and that of G2/M phase cells was significantly increased compared with those in the control group.2） Diallyl trisulfide down regulated Bcl expression while up-regulated bax expression.

结果：MTT显示大蒜素能抑制人结肠癌 HT29细胞的增殖，并具有时间-剂量依赖性；流式细胞术测定结果显示10 μg/ml大蒜素可明显诱导HT29细胞凋亡，并将细胞周期阻滞在G2/M期；可降低癌基因蛋白Bcl表达，而促进Bax表达。

The most of the product became soluble from inclusion body by lowering induction temperature. At the same time the gene was cloned into vector pMAL-p2X, which was translated in E. coli TB1 and expressed a fusion protein. We optimized the induction time and achieved high expression. But there was no obvious product secreted in the periplasm extract. Almost all fusion protein is expressed soluble in cytoplasm.

利用融合型表达载体pTYB11-EFE融合型表达，对诱导剂IPTG的浓度和诱导温度进行了优化，通过降低诱导温度使产物由包含体变成可溶性融合型产物；利用融合型分泌表达载体pMAL-p2X构建了表达载体pMAL-p2X-EFE-7~#，对诱导时间进行了优化，获得了较高的表达量，但发现产物没有明显的分泌表达，绝大部分是以可溶形式存在于胞内。

Estrogen deficiency can increase the mRNA expression of MMP-1、MMP-3 and IL-1β in OA synovia tissue. Estrogen could inhibit the mRNA expression of MMP-1、 MMP-3 and IL-1β in OA synovia tissue. Progestin could even decrease this expression. Estrogen could slightly increase the mRNA expression of TIMP-1. It is suggested that low dose of estrogen supplement play a protective effect on OA cartilage, and progestin could increase this protective effect.

雌激素缺乏可使OA关节滑膜中MMP-1、MMP-3和IL-1β mRNA表达增加，雌激素对关节滑膜组织中MMP-1、MMP-3和IL-1β mRNA表达有抑制作用，孕激素可增强雌激素的这一抑制作用，雌激素对TIMP-1有轻度升高作用，提示低剂量雌激素对OA关节软骨有一定保护作用，而加用孕激素这一保护作用增强。

Systematic aerobic exercise upregulates expression of enzyme genes concerning tricarboxylic acid cycle, and downregulates expression of genes concerning muscle protein synthesis and sphingolipid. It is suggested that aerobic exercise is good to protect human nerves' integrity, exerts positive action on anti-aging and accelerate the aerobic metabolism of lipid materials in vivo.

有氧运动可使三羧酸循环相关酶基因表达上调，肌肉蛋白合成相关基因和神经鞘脂类相关基因表达下调，提示有氧运动有助于保护神经细胞的完整性，对抗衰老有积极作用，同时可加速体内脂类物质有氧代谢。

Of CBMC can be purified by Mini-MACS as CD34〓 stem cells. B. The number of CD34〓 stem cells can expand to 40.24±9. 86 fold after 14 days. C. No matter in the expression of CD1a, CD80, CD86, and HLA-DR, or in the function of stimulating xenogenous lymphocyte proliferation, there was no difference between CD34+ stem cell DCs or monocyte DCs. D. The percentage of CD3〓CD56〓 cells is the same in CIK cells co-culture with DCs transfected with SKOV3 RNA, CIK cells co-culture with DCs, and CIK cells. E. The expansion rate of CIK cells can be accelerated by co-culturing with loaded or unloaded DCs. However, the expansion rate between loaded or unloaded group is the same. F. The strongest cytotoxicity against SKOV3 cell line was achieved by CIK cells co-cultured with DCs loaded with SKOV3 RNA.

结果：1、Mini-MACS分选系统可自CBMC中提取0.78±0.31%的CD34〓细胞；2、体外培养14天后可获得原始CD34〓细胞量40.24±9.86倍的细胞；3、不论在CD1a、CD80、CD86及HLA-DR的表达上，或是刺激异体淋巴细胞增生的功能上，脐带血CD34〓细胞与单核细胞来源的DC都没有差别；4、CIK细胞中CD3〓CD56〓双阳性的表达率在与RNA转染DC共培养的CIK细胞组、与DC共培养的CIK细胞组及单纯CIK细胞组3组间比较无差异；5、脐带血CIK细胞增殖显著，培养14天时可扩增18.18±5.59倍，培养21天时可扩增35.02±6.30倍；5、与未转染或转染DC共培养的CIK细胞在培养第14天后增殖速率大于单纯CIK细胞。

These results suggested that the function of each function domain in HIV 1 LTR exhibited distinct characteristics on gag gene expression in vaccinia virus:(1) The function of intact LTR on gag gene expression varied with the upstream poxvirus promoter;(2) NR sequence neither decreased nor increased the Gag protein exprssion level;(3) Enhancer sequence might not be recognized by recombinant vaccinia virus expression system;(4) TAR sequence enhanced Gag protein expression effectively;(5) U5 region and its downstream non translated sequence had little effect on

免疫印迹和免疫酶试验检测均表明，6株重组病毒的Gag蛋白表达量因LTR不同而有明显差异，表明HIV1的LTR及其下游基因置于痘病毒启动子控制下，在痘苗病毒中表达时有下述特点：（1）不同的痘苗病毒启动子与全长LTR相互作用，对gag基因表达有显著不同的调控效果；（2）NR序列对Gag蛋白表达没有明显影响；（3）EN序列不能被重组痘苗病毒表达系统识别；（4）TAR序列可提高Gag蛋白的表达量；（5）U5区及下游非翻译序列不影响Gag蛋白的表达。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。