可表达的

Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I，并成功地在原核细胞E.coli进行了重组表达，筛选出稳定表达的工程菌株DH5α，摸索了稳定表达的条件，摸索了包涵体的洗涤、变性和复性条件，摸索出了复性蛋白Src I的纯化工艺，纯化过程简单，经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化，就可获得了高纯度的重组蛋白样品(纯度达99％以上)，该纯化方法适合重组蛋白的大量纯化，为Src I的性质、结构和功能研究奠定了良好的基础。

In order to correct these irrational actions, we must build a mechanism to induce the individual expression of interests into a controllable track, fully exerting the influences of policy, interest, value, role and model, etc. Therefore, the rational expression of interest subjects can be achieved in a legitimate way.

针对这些非理性行为必须构建一个引导机制，充分发挥政策引导、利益引导、价值引导、榜样及角色引导的作用，将个体的表达行为纳入到可控制的轨道上来，引导利益表达主体通过合法途径来表达合理利益诉求。

On the basis of Earth surface space using reference ellipsoid as height basis, map space using Gauss-Kruger projection plane as height basis, a viewpoint is proposed that map projection actually establishes corresponding relationship between the height bases of two different spaces above. From the viewpoint of projection and visible representation, the relationships among earth surface space, RS imagery space and map space are analyzed; the differences among different representations of earth surface space are identified theoretically; virtual reality is applied to the representation of land surface space, and a prototype of real 3D virtual reality system based on spatial information is developed. By means of digital simulation, virtual reality representation of land surface space in visual image space is realized.

在以参考椭球面为高程基准面表征地表空间、以高斯-克吕格地图平面为高程基准面表征地图空间的思想基础上，提出了地图投影实质上是建立两个空间的高程基准面之间关系的观点；从投影和可视表达的角度，分析了地表空间、遥感图像空间、地图空间之间的关系，从理论上区分了对地表空间的不同表达方式之间存在的差别；将虚拟现实技术应用于地表空间的表达，研制了空间信息三维虚拟现实系统原型，利用数字模拟技术在视像空间实现了对地表空间的真三维虚拟现实表达。

To understand whether the GST can protect plant from the damage of ultra-violet radiation,a UV inducible GST gene was isolated from an Arabidopsis cDNA library.The plant expression vector containing the GST cDNA was constructed and transferred into Arabidopsis thaliana plants by the Agrobacterium-mediated vacuum method.Molecular genetic analyses showed that the overexpression of the UV inducible GST could increase the tolerance of the transgenic plants to UV radiation

为了阐明GST在紫外辐射下是否对植物有保护作用，以紫外强烈诱导表达的GST cDNA为探针，筛选拟南芥cDNA文库，获得了这种GST的全长cDNA；利用此cDNA构建植物表达载体，并通过农杆菌介导法转化拟南芥，使其在拟南芥中得到大量表达；通过对转基因植株的紫外辐射耐性分析，证实了该GST的过量表达可明显增强拟南芥对紫外辐射损伤作用的耐受性。

ERα is the major subtype of estrogen receptor in vaginal tissue of rats. ERα and ERβ expressions are down-regulated in the vaginal tissues after ovariectomy. Our findings show that estradiol valerate can up-regulate the expression of ERα but ERβ.

ERα是大鼠阴道优势表达的雌激素受体亚型；去势后阴道ER亚型表达下调，以ERβ明显；戊酸雌二醇可明显上调去势大鼠阴道ERα的表达，但不上调ERβ的表达。

Two idiotype TCR Vα1-pIRES-TCR Vβ8 recombinant eukaryotic vectors weredeveloped successfully, and the expression of both idiotypic TCR mRNA and proteincould be detected in transferred A549、K562 and Molt4 cells.

2成功构建了两组TCR Vα1-pIRES-TCR Vβ8表达载体，该表达载体转染A549、K562和Molt4细胞后，可表达相应的mRNA和蛋白。

The immunohistochemistry showed that accompanied with SDNSF mRNA upregulation, the nestin-positive cells showed erupted roots, migrated peripherad and proliferation on the 8-day slice.

结果 RT-PCR检测SDNSF mRNA在正常大鼠脊髓中的表达，损伤后4天SDNSF 的mRNA表达上升，损伤8天到达高峰，此后SDNSF 的mRNA表达逐渐减少，到16天恢复到正常水平；脊髓切片原位杂交结果发现SDNSF的 mRNA 阳性细胞主要分布于脊髓灰质细胞中，可能是神经元细胞，结果表明正常脊髓可表达SDNSF；脊髓损伤后8天，原位杂交显示SDNSF阳性细胞明显增多。

The immunohistochemistry showed that accompanied with SDNSF mRNA upregulation, the nestin-positive cells showed erupted roots, migrated peripherad and proliferation onthe 8-day slice.

结果 RT-PCR检测SDNSF mRNA在正常大鼠脊髓中的表达，损伤后4天SDNSF的mRNA表达上升，损伤8天到达高峰，此后SDNSF的mRNA表达逐渐减少，到16天恢复到正常水平；脊髓切片原位杂交结果发现SDNSF的mRNA阳性细胞主要分布于脊髓灰质细胞中，可能是神经元细胞，结果表明正常脊髓可表达SDNSF；脊髓损伤后8天，原位杂交显示SDNSF阳性细胞明显增多。

These results indiacated that tranferring macrophages combinedwith cytokines could be a new adoptive immunotherapy protocol foradvanced cancer,and rTNF used in vivo locally could induce andactivate macrophages to kill tumor cells.Monocytes when activatedunderwent a series of phenotypic and functional changes includingthe expression of IL-2R which may provide an important immunoregu-latory pathway.The presence of lectin-like molecules on thesurface of monocytes and tumor cells may bring theeffector/target cells together,thus facilitating the inductionof apoptosis in target cells by triggering the production ofcytolytic factors and the modification of target of targer cell surface anti-gens.(such as HLA-DR).

综合以上结果可以得出以下结论：转输巨噬细胞并结合细胞因子的联合应用是肿瘤继承性免疫中的又一新的、有潜力的方法；抗肿瘤细胞因子rTNF可通过激活巨噬细胞在体内抑制对其不敏感的肿瘤细胞的生长；人单核细胞在一定条件下激活后，可表达IL-2R，进而一方面增强其自身对IL-2的敏感性，另一方面在体内也具有调节T细胞功能的作用；单核细胞和肿瘤细胞上存在的凝集素类受体可促使两种细胞之间在凝集素介导下相互接近，并诱发单核细胞产生细胞毒因子，以及通过调节靶细胞上表面抗原的表达，促进诱导靶细胞的细胞凋亡。

Under the transmission electron microscope, we found adjacent cells in rat MSCs with mHCN4 gene and neonata atrial cells in coculture often had intimately apposed interdigitating cell membranes.

经检测mHCN4基因修饰大鼠MSCs可表达Cx43，其表达水平明显较GFP组及未转染组高，与心房肌细胞的Cx43表达水平无明显差异。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐