可表达的

After thermal induction, no specific recombinant protein band in SDS-PAGE was found, but G-CSF activity was detectable. Therefore, a new recombinant plasmid pBVHG2 expressing hG-CSF hybrid protein with additional 8 amino acids which could be cut off specifically with the help of mucosal enterokinase at the N terminus of hG-CSF was constructed.

含此质粒菌株虽然表达菌体裂解后可测得明显的生物学活性，但SDS-PAGE仍未见特异表达产物带；因此，再应用相同方法，在hG-CSF cDNA突变体5′端增加24核苷酸对的FLAG肽编码序列，构建了hG-CSF杂交蛋白（hG-CSF天然蛋白N末端增加8aa的FLAG肽，后者可由肠激肽酶切除）的表达质粒pBVHG2。

All the patients had mucocutaneous pigmentation, gastrointestinal polyps, abdominal pain, anemia, and so on. All the patients were examined by endoscopy, polyps were removed by electrocautery snear under endoscopes, and 3 cases underwent enterotomy operation because of intestinal obstruction and intussusception.

结直肠癌组织和正常结肠黏膜广泛表达ER-β，正常结肠上皮主要呈核染，而结直肠癌组织主要为胞浆染色，ERβ在结肠上皮细胞表达，尤其在处于腺体底部的结肠隐窝大量表达，ERβ还可在内皮细胞和肌细胞上表达。

For targeted and regulable gene transcription, tissue or tumor specific promoters and some manual regulatory systems are used to regulate therapeutic gene expression.

为了实现基因的靶向可调控表达，组织或肿瘤特异性的启动子和人工合成的可调控表达系统被用来调控治疗基因的表达。

This suggests that CaHIRA plays a role in the early embryo development and the late stages. The different expression characters of hira gene in these fish species may be related to the different reproduction models. The research on Drosophila has indicated hira gene related to sperm nucleus decondensation during fertilization. The high expression of hira gene before fertilization is prepared for the sperm nucleus decondensation and the formation of male prenucleus because colored crucian carp is bisexual reproduction. However, the sperm nucleus is not decondensated during the fertilization and is only to activate egg nucleus in gynogenetic gibel carp.

在彩鲫中，从未受精卵到胚胎发育的各个时期均可检测到表达，但表达量发生明显变化，受精后表达量迅速下降，直到原肠胚期表达量有所上升，并一直保持到出苗。hira基因在两亲缘种成熟卵子受精前后表达情况不同，可能与两种鱼不同的生殖方式有关。

objective effects of plant tannins,chebulinic acid and tellimagrandin i on chemically induced hemoglobin synthesis in k562 cells were investigated.methods the hemoglobin synthesis situation was assayed with benzidine staining.erythroid antigens glycophorin a expression on the surface of k562 cells was labeled by direct immunofluorescence using fluorescein isothiocyanate-conjugated anti-gpa antibodies.then flow cytometric analysis was performed to detect gpa expression levels on surface of the cells.results both chebulinic acid and tellimagrandin i could inhibit the hemoglobin synthesis of butyric acid and hemin-treated k562 cells in a concentration-dependant manner.however,the ba-induced k562 cells were more sensitive to two tannins than heroin-induced cells.conclusion chebulinic acid and tellimagrandin i have inhibitory effect on the erythroid differentiation.

目的 研究可水解单宁诃子酸和特里马素i对氯化高铁血红素和丁酸钠诱导k562细胞红系分化的影响。方法四甲基偶氮噻唑蓝法分析诃子酸和特里马素i对k562细胞生长的影响，联苯胺染色法检测血红蛋白合成情况，应用免疫荧光和流式细胞术检测血型糖蛋白a在细胞表面的表达。结果诃子酸和特里马素i在0.04～0.09？mmol／l浓度下均可显著抑制k562细胞的生长增殖。2种单宁化合物(0.002～0.01？mmol／l)可显著抑制丁酸钠诱导的k562细胞血红蛋白合成，2种单宁化合物(0.01～0.05？mmol／l)还可显著抑制氯化高铁血红素诱导的血红蛋白合成，特里马素i(0.01？mmol／l)还抑制丁酸钠诱导的gpa在细胞表面表达。结论诃子酸和特里马素i对红系分化有抑制作用。

Results The levels of HSP 70 mRNA and HSP 70 protein expression were significantly different among the 5 groups. Ketamine induced marked HSP 70 mRNA and HSP 70 protein expression in the posterior cingulated cortex. Propofol itself did not induce HSP 70 gene expression in this brain region. Propofol significantly inhibited ketamine-induced HSP 70 mRNA and HSP 70 protein expression in the posterior cingulate cortex in a dose-dependent manner.

结果氯胺酮可明显诱导HSP70 mRNA与HSP70蛋白在大鼠后扣带回皮质区的表达；异丙酚自身不能诱导HSP70基因的表达；预先给予异丙酚可显著抑制氯胺酮诱导的HSP70 mRNA和HSP70蛋白在这一区域的表达，且抑制效应呈剂量依赖性。

The high expression of mmp-2 and cd44v6 were positively correlated with lymph node metastasis and fibromembranous infiltration （p＝0.011, p＝0.034和p＝0.022, p＝0.001）.the expression of mmp-2 was positive relation to that of cd44v6 （r＝0.427,p＝0.002）.conclusion the detection of expression about mmp-2 and cd44v6 in esophageal carcinoma may be helpful to judge the malignant behavior,metastasis and progronsis.

食管癌组织中mmp-2表达与cd44v6表达呈正相关（r＝0.427，p＝0.002）。结论 mmp-2和cd44v6表达是食管癌发生与发展的重要促进因子，其过度表达与食管癌浸润转移密切相关。检测mmp-2和cd44v6蛋白表达可作为判断食管癌预后的客观指标。

Results:(1) FSN can inhibit secondary joints swelling and multi-arthritis evidently, improve the whole condition of rats; at the same time, it can also lighten the synovial inflammation and hyperplasia of lesion joints distinctly, and prevent the joint cartilage and bone from destruction; the collective efficiency of FSN is better than TWP.(2) FSN can raise AA rats low LTT of spleen to normal nearly, remedy the disorder of Th / Ts and Thl / Th2 cells balance in peripheral blood, restrain exorbitant TNF- a ? IL-1 produced by PM O , thereof exert anti-inflammatory and immunoregulation effect.(3) Inside the lesion joints, FSN can depress abnormal hyper-expression of TNF- a mRNA and NF-kB in synovial tissues, as well as advance the expression of Caspase-3 (a proteolytic enzyme of apoptosis), reduce synovial immflamination and proliferation.(4) FSN can lower the expression of VEGF in synovial tissue,reduce neogenetic veins, so inhibit the growth of pannus tissue and the damage of cartilage and bone by that.(5) The above effect of FSN are in proportion to its dosage.Conclusions: FSN has inhibitory effects on symptom and condition of experimental RA, which is better than TWP as a whole.

结果：(1)FSN有明显的抗炎作用，可显著抑制AA大鼠足跖肿胀与多关节炎，改善大鼠的全身情况，同时明显减轻病变关节滑膜炎症与增生，防止关节软骨及骨质的破坏，其综合作用优于TWP；(2)FSN还能使AA大鼠低下的脾LTT恢复至接近正常，纠正外周血中T细胞亚群Th／Ts及Th1／Th2平衡紊乱，抑制大鼠PHφ过高的TNF-α、IL-1分泌，而发挥抗炎和免疫调节作用；(3)在病变关节局部，FSN能显著抑制AA大鼠滑膜细胞异常增高的TNF-αmRNA的表达，降低滑膜组织中NF-kB表达，并增加凋亡蛋白酶Caspase-3表达，从而抑制滑膜的炎症与增殖；(4)FSN还可下调滑膜组织中VEGF表达，减少血管新生，由此抑制血管翳的形成及其对软骨和软骨下骨的侵蚀；(5)FSN的上述作用皆呈现一定的量效依赖关系，高剂量组作用优于低剂量组。

FSN can raise AA rats low LTT of spleen to normal nearly, remedy the disorder of Th/Ts and Th1/Th2 cells balance in peripheral blood, restrain exorbitant TNF-α、IL-1 produced by PMφ, thereof exert anti-inflammatory and immunoregulation effect.(3) Inside the lesion joints, FSN can depress abnormal hyper-expression of TNF-αmRNA and NF-kB in synovial tissues, as well as advance the expression of Caspase-3 (a proteolytic enzyme of apoptosis), reduce synovial immflammation and proliferation.(4) FSN can lower the expression of VEGF in synovial tissue, reduce neogenetic veins, so inhibit the growth of pannus tissue and the damage of cartilage and bone by that.(5) The above effect of FSN are in proportion to its dosage. Conclusions: FSN has inhibitory effects on symptom and condition of experimental RA, which is better than TWP as a whole.

结果：(1)FSN有明显的抗炎作用，可显著抑制AA大鼠足跖肿胀与多关节炎，改善大鼠的全身情况，同时明显减轻病变关节滑膜炎症与增生，防止关节软骨及骨质的破坏，其综合作用优于TWP；(2)FSN还能使AA大鼠低下的脾LTT恢复至接近正常，纠正外周血中T细胞亚群Th/Ts及Th1/Th2平衡紊乱，抑制大鼠PMφ过高的TNF-α、IL-1分泌，而发挥抗炎和免疫调节作用；(3)在病变关节局部，FSN能显著抑制AA大鼠滑膜细胞异常增高的TNF-αmRNA的表达，降低滑膜组织中NF-kB表达，并增加凋亡蛋白酶Caspase-3表达，从而抑制滑膜的炎症与增殖；(4)FSN还可下调滑膜组织中VEGF表达，减少血管新生，由此抑制血管翳的形成及其对软骨和软骨下骨的侵蚀；(5)FSN的上述作用皆呈现一定的量效依赖关系，高剂量组作用优于低剂量组。

Conclusions1、E_2 may induce the apoptosis and Fas,FasL expressions of Jurkat cell,its effect positively related to the dose and treating time of E_2.2、There is intrinsic co-expression of ERαand ERβon Jurkat cell,which can be upregulated by E_2,positively related to E_2 doses.3、E_2 may act as an apoptosis inducer of Jurkat cell via ERβ.4、E_2 can promote the transcriptive activity of NF-κB in Jurkat cell.The increased NF-κB activity may lead to increased apoptosis of Jurkat cell.

结论1、E_2可增加Jurkat细胞的凋亡，并增加Fas、FasL的蛋白表达，且其作用与E_2的剂量和处理时间呈正相关。2、Jurkat细胞上存在ERα、ERβ的表达，且E_2可上调这两种受体的表达，E_2剂量越大，ER表达量越高。3、E_2可能通过ERβ发挥其促进Jurkat细胞凋亡的作用。4、E_2能增加Jurkat细胞中NF-κB的转录活性，Jurkat细胞中NF-κB活性增加有促进凋亡的作用。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。